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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 833 results for: ins

Showing: 681 - 700 of 833 results

  1. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...sensors, and other common neuroscience tools into the brains of model organisms, including the Anikeeva lab’... Plasmid tools for microbiome studies Channel rhodopsins for optogenetics Viral Vectors 101: Chemogenetics...

  3. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...CRISPR than just designing a repair template that contains your desired edit, and I want to make sure that...long ssDNA template to increase efficiency of insertion and gene replacement. Care to share your favorite...

  6. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...field of science you are communicating from. For instance, a plumber, a physicist, an ambassador to Spain...exclude people outside of the original recipients. Instead, it targets a specific audience - giving consistency...

  7. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...embryogenesis. My interests, though, have proven unruly. Instead of settling down into a singular focus on molecular... general terms on a couple of very broad topic domains, but each day brings in new and surprisingly diverse...

  8. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...partnerships with universities, companies, hospital institutions and funding bodies. Don't shy away from the ...company advancement. Be relentless The world is against you.  As I mentioned, you start at a disadvantage...

  9. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...help unlock the potential of modern sequencing instruments by enhancing the efficiency and simplicity of...completely unnecessary for sequencing multi-kb DNA inserts and larger targets. The NGS readout also reveals...

  10. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ... the DNA prep is larger. And since the library contains many plasmids, care must be taken to ensure that...plasmids in the library are identical except for the insert; there’s no need to sequence other parts of the...

  13. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...models for immunogenicity prediction of therapeutic proteins. European journal of pharmaceutics and biopharmaceutics...biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 130, ...

  14. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...never finish a course of antibiotics, choosing instead to save a few days’ worth of capsules to self-medicate...much of the information gained from that research remains inaccessible, behind paywalls and in technical ...

  15. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...need to present yourself using a resume. Indeed, instead of having all the pages in a Curricula Vitae to...career, she transitioned into a communications role instead after she completed her MSc in Experimental Medicine...

  16. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al., 2014). It is a dual-vector system...viruses are popular, pAAV-hSyn-DIO-hM4D(Gi)-mCherry contains a double floxed human muscarinic receptor 4 (Gαi-coupled...

  17. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    .... Projects such as the OpenFlexure Microscope (Collins, et. al., 2020), the miCube, SQUID (Hongquan, et...and low-cost optical setups. References Joel T. Collins, Joe Knapper, Julian Stirling, et. al, "Robotic...

  18. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...months: Lentiviral preps for expressing SARS-CoV-2 proteins ChR2 for targeted expression DREADDs GFP dependent... quickly shared plasmids expressing SARS-CoV-2 proteins at the beginning of April. Now, we've turned some...

  20. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ... coexpression of light (green) and heavy (blue) chains as driven by two CMV promoters (orange). Figure... to WGS the lab’s entire hybridoma collection. Instead, the Trimmer Lab moved to direct functional validation...
Showing: 681 - 700 of 833 results