We narrowed to 714 results for: met
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TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....have been continuously pursuing new and better methods to peer into the mysterious, microscopic world ...
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Magnetic Control of Proteins: More than a Dream
TypeBlog PostPublishedSept. 24, 2024, 1:15 p.m.... 2016; Duret et al., 2019). But the field has sometimes seen controversy, and the interactions of organic... -
Hot Plasmids: Winter 2025
TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m....possible with conventional electrophysiological methods, it has remained difficult with GEVIs. Not only... -
Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m.... inversions and large deletions. Many of these methods rely on Cas9 or a derivative of Cas9, but the ever-expanding... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog PostPublishedOct. 23, 2018, 11:49 a.m.... terminal repeat sequences. Human gene therapy methods, 23 1, 18-28. PubMed PMID: 22428977. 2. Choi, V.W... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog PostPublishedMarch 28, 2018, 6:52 p.m....near your insertion site. Base editing, another method to create point mutations, also has a strict editing... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog PostPublishedMay 26, 2016, 2:30 p.m....thermostable periplasmic endonuclease that can sometimes co-purify with your plasmid DNA and lead to degradation... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....the protein's behavior in cells, in solution or something in between, you are using a tag with a serious... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m.... citation for you to use in your Materials and Methods and References sections when citing the use of ... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog PostPublishedSept. 23, 2014, 3:05 p.m.... to include your improvements, or maybe even a methods paper. The problem with this is that it may take... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m.... any other ancestry group. With the existing methodology, they estimate that the African ancestry-associated... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....activate the immune system, and of the infection to sometimes result in cell death, has led to interest in using... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog PostPublishedDec. 3, 2019, 11:38 a.m....their plasmids are available from Addgene in the Methods & Materials or elsewhere in their papers. Maybe... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog PostPublishedJan. 11, 2018, 3:29 p.m....responsibilities. If you are in charge of completing many meticulous protocols or tasks, you’ve probably developed... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m.... screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID: 25075903. PubMed... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog PostPublishedOct. 14, 2015, 2:30 p.m.... with gRNA sequence. Both groups used multiple methods to examine Cpf1 off-target editing. First, they... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m.... associated with the library and reading their methods sections to see what worked. Ready, optimize, ... -
Hot Plasmids Spring 2024
TypeBlog PostPublishedMay 23, 2024, 1:15 p.m....screens By Emily Bentley Building on their CROPseq method for optical pooled CRISPR screening, Paul Blainey... -
Tips for Writing a Good Cover Letter
TypeBlog PostPublishedMay 21, 2024, 1:30 p.m....skills for the position]. I am enthusiastic about [something that you love and is important for the job]. ... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog PostPublishedNov. 21, 2024, 10:47 p.m....and its parts that helps the viewer visualize something that cannot be seen with the naked eye. In 2023...