We narrowed to 718 results for: tat

1. Transferable Skills: Negotiation

   Type

   Blog Post

   Published

   March 3, 2022, 2 p.m.

   ...more important to you and why? What are their expectations/needs and how are they different from mine? ...

2. Viral Vectors 101: Voltage Indicators

   Type

   Blog Post

   Published

   Jan. 30, 2024, 2:15 p.m.

   ...of the previously described systems. Several adaptations of this method have been to tag, cage, uncage...

3. Google Forums Round Up: First Impressions of NgAgo

   Type

   Blog Post

   Published

   Aug. 4, 2016, 2:30 p.m.

   ...sequence of DNA. Second, NgAgo target specificity is dictated by a DNA guide sequence rather than an RNA guide...

4. 10 Basic tips for mammalian cell culture

   Type

   Blog Post

   Published

   July 12, 2018, 1:09 p.m.

   ...their comfort zone around 37 °C and 5% CO2 and get agitated with sudden changes in temperature (especially...

5. AAV Vector Quality Control: Going the Extra Mile with NGS

   Type

   Blog Post

   Published

   Sept. 12, 2017, 1:44 p.m.

   ...is Addgene plasmid #44362. Method validation, limitations, and future plans How did AAV-68544 become contaminated...

6. Fluorescent Proteins 101: Fluorescent Protein Timers

   Type

   Blog Post

   Published

   May 4, 2017, 2:30 p.m.

   ...Associated Plasmids from Subach et al. Protein Excitation (nm) Emission (nm) Brightness pKa Maturation ...

7. Tips to Make the Most of a Scientific Conference

   Type

   Blog Post

   Published

   May 23, 2019, 12:26 p.m.

   ...themselves and then ask questions. Practice your presentation for both instances. If your poster material ...

8. Plasmids 101: Knockout/Knock-In Plasmids

   Type

   Blog Post

   Published

   Dec. 1, 2016, 3:30 p.m.

   ...possible to insert, or knock-in, any gene, tag, or mutated exon into the genome. For this purpose, the sequence...

9. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

   Type

   Blog Post

   Published

   May 22, 2015, 4:58 p.m.

   ...method for converting heterozygous to homozygous mutations. Science aaa5945 (2015). doi:10.1126/science.aaa5945...

10. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type

    Blog Post

    Published

    July 1, 2025, 1:15 p.m.

    ..., routine machine maintenance, and critical interpretation of the results.  If you see evidence of contamination...

11. Antibodies 101: Producing Recombinant Antibodies

    Type

    Blog Post

    Published

    Aug. 13, 2024, 1:15 p.m.

    ...Do not drop: mechanical shock in vials causes cavitation, protein aggregation, and particle formation....

12. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type

    Blog Post

    Published

    Sept. 6, 2018, 12:02 p.m.

    ...disulfide-activated ligands. Due to the concerns of off-target mutations and transgene integration in crops, delivery of...

13. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    Published

    Feb. 27, 2018, 2:04 p.m.

    ... on the Addgene Blog DIY DNA ladders from Penn State University Use Nanobodies to Control Protein Activity...

14. Transferable Skills Guide: Identifying Your Transferable Skills

    Type

    Blog Post

    Published

    Jan. 11, 2018, 3:29 p.m.

    ...scientists' transferrable skills. In describing the limitations of the study, the authors noted that “Many trainees...

15. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type

    Blog Post

    Published

    March 17, 2016, 2:30 p.m.

    ...independently of other viral proteins simplifying the interpretation of results. This system can be used to develop...

16. The Importance of a Fun Workplace: Company Culture at Addgene

    Type

    Blog Post

    Published

    Sept. 3, 2015, 2:30 p.m.

    ...environment Team building events like Escape the Room or Habitat for Humanity take you and your co-workers out of...

17. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    Published

    March 20, 2014, 3:04 p.m.

    ...decent meal, fold their laundry, take public transportation, call a cab and remember their own school assignments...

18. QC Sequencing Technologies at Addgene

    Type

    Blog Post

    Published

    July 15, 2025, 1:15 p.m.

    ...time-consuming, depending on the library prep and instrumentation used.  Here at Addgene, we sequence every plasmid...

19. Plasmids 101: Shuttle Vectors

    Type

    Blog Post

    Published

    May 20, 2025, 1:15 p.m.

    ...the researcher, Zeocin has been shown to cause mutations even in cells with the resistance gene, so it ...

20. CRISPR 101: Any Base Transversion Editors

    Type

    Blog Post

    Published

    April 1, 2025, 1:15 p.m.

    ... edits. Their final tool, ACBE-Q (for an N108Q mutation in the deaminase), produced an average A → C product...