We narrowed to 711 results for: tat

1. Production of Virus in Insect Versus Mammalian Cells

   Type

   Blog Post

   Published

   Nov. 5, 2024, 8:32 p.m.

   ...eukaryotic organisms. Typically, what system is used is dictated by the specific type of virus you are trying to...

2. Plasmids 101: Knockout/Knock-In Plasmids

   Type

   Blog Post

   Published

   Dec. 1, 2016, 3:30 p.m.

   ...possible to insert, or knock-in, any gene, tag, or mutated exon into the genome. For this purpose, the sequence...

3. Google Forums Round Up: First Impressions of NgAgo

   Type

   Blog Post

   Published

   Aug. 4, 2016, 2:30 p.m.

   ...sequence of DNA. Second, NgAgo target specificity is dictated by a DNA guide sequence rather than an RNA guide...

4. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

   Type

   Blog Post

   Published

   May 22, 2015, 4:58 p.m.

   ...method for converting heterozygous to homozygous mutations. Science aaa5945 (2015). doi:10.1126/science.aaa5945...

5. CRISPR 101: Ribonucleoprotein (RNP) Delivery

   Type

   Blog Post

   Published

   Sept. 6, 2018, 12:02 p.m.

   ...disulfide-activated ligands. Due to the concerns of off-target mutations and transgene integration in crops, delivery of...

6. Plasmids 101: Secondary Nanobody Toolbox

   Type

   Blog Post

   Published

   Feb. 27, 2018, 2:04 p.m.

   ... on the Addgene Blog DIY DNA ladders from Penn State University Use Nanobodies to Control Protein Activity...

7. Antibodies 101: Producing Recombinant Antibodies

   Type

   Blog Post

   Published

   Aug. 13, 2024, 1:15 p.m.

   ...Do not drop: mechanical shock in vials causes cavitation, protein aggregation, and particle formation....

8. Transferable Skills Guide: Identifying Your Transferable Skills

   Type

   Blog Post

   Published

   Jan. 11, 2018, 3:29 p.m.

   ...scientists' transferrable skills. In describing the limitations of the study, the authors noted that “Many trainees...

9. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

   Type

   Blog Post

   Published

   March 17, 2016, 2:30 p.m.

   ...independently of other viral proteins simplifying the interpretation of results. This system can be used to develop...

10. The Importance of a Fun Workplace: Company Culture at Addgene

    Type

    Blog Post

    Published

    Sept. 3, 2015, 2:30 p.m.

    ...environment Team building events like Escape the Room or Habitat for Humanity take you and your co-workers out of...

11. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    Published

    March 20, 2014, 3:04 p.m.

    ...decent meal, fold their laundry, take public transportation, call a cab and remember their own school assignments...

12. Hot Plasmids - August 2020

    Type

    Blog Post

    Published

    Aug. 18, 2020, 1:15 p.m.

    ...calcium indicator jGCaMP7, that are optimized for excitation wavelengths above 1,000 nm and enable two-color...

13. Viral Vectors 101: Pseudotyping

    Type

    Blog Post

    Published

    April 20, 2021, 2:30 p.m.

    ... Q, Liu Q, Huang W, Li X, Wang Y (2017) Current status on the development of pseudoviruses for enveloped...

14. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type

    Blog Post

    Published

    July 23, 2019, 12:46 p.m.

    ... PHP.B and the C57BL/6J version of LY6A, but a mutation in PHP.B’s unique capsid loop disrupted binding...

15. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    Published

    March 12, 2019, 1:37 p.m.

    ... they can understand and are engaged by your presentation, then you’re on the right track. Furthermore...

16. How to Be an Excellent Trainee

    Type

    Blog Post

    Published

    Sept. 26, 2023, 1:15 p.m.

    ...are going to be there – undergraduate students, rotation students, joining a new lab at any career stage...

17. Plasmids 101: Shuttle Vectors

    Type

    Blog Post

    Published

    May 20, 2025, 1:15 p.m.

    ...the researcher, Zeocin has been shown to cause mutations even in cells with the resistance gene, so it ...

18. CRISPR 101: Any Base Transversion Editors

    Type

    Blog Post

    Published

    April 1, 2025, 1:15 p.m.

    ... edits. Their final tool, ACBE-Q (for an N108Q mutation in the deaminase), produced an average A → C product...

19. Twenty Years of Sharing: Expanding Accessibility

    Type

    Blog Post

    Published

    Nov. 21, 2024, 10:47 p.m.

    ...darker blue indicates more views from that country. Stats and figures are helpful, but our favorite measure...

20. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type

    Blog Post

    Published

    July 30, 2024, 1:15 p.m.

    ...to the research community. It does, however, necessitate us first identifying broadly useful vectors, ...