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  1. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...world. To support this pace of research, new, centralized databases and centers to support the sharing ...community response initiatives. Accelerate the translational impact of research Universities have also been...creating research synergies and accelerating the translational impact of research activities. By streamlining...
  2. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ... are trained to be good at learning new things—this is a strength you can draw on and demonstrate in your...often is the most effective tactic a scientist in training can adopt to ensure opportunities in the future...
  3. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...near-complete excision of the stop sequence. In contrast, Dre recombinase, the close relative of Cre, displayed... were inherited normally and thus nontoxic. In contrast, 0/79 offspring carried both Cre and GAL4, further...induced by Cre recombinase in mammalian cells. Loonstra A, Voojis M, Beverloo HB, Al Allak B, van Drunen...
  4. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    Published
    Sept. 15, 2014, 6:05 p.m.
    ...Polish sub-dialect making their Skype communications extra fun) who shared first authorship on their seminal...this type of experimentation to the eventual translation to the clinic and cures. There is no doubt that...forever. He emphasized the potential of this extraordinary breakthrough. In fact, the panelists agreed ...
  5. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...have: working to deadlines, writing up clear and attractive reports, being good at presentations and of course...studies. They suggest that you make time for extracurricular activities both to develop new skills and to...information about the benefits of pursuing extracurricular activities. Success evolves from passion True...
  6. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...sonification. Though it helps capture weaker or more transient DNA-protein interactions, the reliance on fixation...McKay lab we study how differential access to transcriptional regulatory elements is controlled during development...permeabilize cells rather than to rely on nuclei extraction. They report that this change offers a significant...
  7. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...over Na+ and rapid photocurrent kinetics. They demonstrate the use of HcKCR1 as an optogenetic silencer ...of HcKCR1 in mouse neurons. D) Membrane voltage traces of action potentials in a HcKCR1-expressing mouse...promising avenues for future engineering to shift the spectra and improve the performance of HcKCRs. Find HcKCR...
  8. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...blot and immunofluorescence. GST  Glutathione S-Transferase (GST) is a 26 kDa tag which can be isolated with... glutathione resin, eliminating the need for an extra clean up step of the tag – a handy feature. If you...fusion proteins from E. coli is generally more straightforward and of a higher purity than mammalian purifications...
  9. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...or A:T->G:C edits in DNA without causing double-strand breaks. Our new page shows you base editing plasmids...certainly taken notice! Our newest page in the Transcriptional Regulation category already has five blue flame...with eSpCas9, SpCas9-HF1, & HypaCas9 Day 10: Transcriptional regulation: Truncated gRNAs for Regulating ...
  10. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    Published
    Oct. 3, 2017, 12:11 p.m.
    ...(get tips for your next science talk). I do illustrations for summarizing mechanisms and applications ...part of Hans’ workload. “I am currently getting abstracts and plans together for my next 4 webinars—I have...communication with the presenters, and editing of abstracts and speaker biographies.” During the week of ...
  11. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...managers are rarely chosen because they have demonstrated skill at managing and this is especially true...getting your PhD or doing a postdoc few science trainees will have opportunities to work on Emotional Intelligence...diversity of responsibilities, which might include training or teaching others, research, and policy development...
  12. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...it another way. You’ll know you are on the right track if they start asking you questions. Remember that...out how they got where they are. 8)   Look into training from an organization such as COMPASS. Although...their science matter. If you aren’t ready for a training or one isn’t available yet, order a copy of Nancy...
  13. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...Cambridge, Massachusetts guidelines and wear an extra sweater so we don’t have to turn up the heat when...saving freezer space).  The procedure becomes less attractive when noting that one step involves boiling the... volume.  So, I designed my own gel box (see illustration above) and bribed the guys in the hospital machine...
  14. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...A scientist-in-training will spend 10 or more years with a small number of formal advisors learning how... will be ones that help them succeed after the training period. While there are many aspects to choosing...choosing a supportive, positive role model. By contrast, as a postdoc, you should choose a field that ...
  15. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ... sensor AAV GABA is the primary inhibitory neurotransmitter in vertebrates, but methods for directly imaging...pGP-AAV-syn-jGCaMP7c variant 1513-WPRE 7c exhibits high contrast between peak fluorescence and resting fluorescence...pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE7c exhibits high contrast between peak fluorescence and resting fluorescence...
  16. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...mentors to listen, guide and support us during training and throughout our careers. An easy way to access...every position, we look for candidates that have demonstrated initiative. In fact, hear from Addgene’s former...Funding For Your Research University of Florida: Training Grants and Other Funding Opportunities L’oreal...
  17. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    Published
    Nov. 12, 2019, 2 p.m.
    ...interested in exploring biological dark matter – large tracts of the microbial tree of life that remained unexplored...night was fun but also very tiring. It feels a bit strange to be using a micropipette and gloves at a campsite...rRNA in bacterial RNA-Seq. This enriches for transcripts of interest without an increase in the number...
  18. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...relevant science."     - Randall Carter, Contracts Administrator Inferior: How Science Got Women Wrong -...Associate Director of Quality Control Jonathan Strange & Mr. Norrell by Susanna Clarke "A quasi-historical...
  19. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...; and sequence of biologic product, mode of administration, and dose (Lu et.al., 2020).  Evaluating a ...of therapeutics in silico. These algorithms are trained on vast amounts of data to calculate the relative... immunogenicity is typically performed using traditional 2D assays with immune cell lines or peripheral...
  20. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...once got a box of 6 perfect, chocolate-covered strawberries for helping with an introduction that helped... have been enough, but I’ll never forget the strawberries. Grand gestures are memorable! Attend events... want to do: Don’t connect via LinkedIn with strangers. It’s a waste of time. People that don’t know ...
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