We narrowed to 694 results for: ust

1. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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   Blog Post

   Published

   May 13, 2025, 1:15 p.m.

   ...experiments involving IDLVs. And remember: You can never trust a lentivirus with secrets... Because they tend to...

2. CRISPR 101: Homology Directed Repair

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   Blog Post

   Published

   Jan. 26, 2023, 2:15 p.m.

   ...Precision Genome Engineering. 2017.  Easi-CRISPR: a robust method for one-step generation of mice carrying...

3. Site Directed Mutagenesis by PCR

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   Blog Post

   Published

   Aug. 2, 2016, 2:30 p.m.

   ...polymerase manufacturers’ PCR protocol. Be sure to adjust the extension time to match the size of your template...

4. Plasmids 101: Dimers and Multimers

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   Blog Post

   Published

   Jan. 10, 2023, 2:15 p.m.

   ...features remain intact, plasmid multimers function just as well as monomers. However, due to their larger...

5. Antibodies 101: The Basics of Western Blotting

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   Blog Post

   Published

   March 8, 2021, 2:41 p.m.

   ...band. It is important to note, however, that you must include a loading control when assessing protein...

6. Building Global Connections with the International Mentorship Program USA-EUROPE

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   Blog Post

   Published

   Nov. 6, 2015, 3:30 p.m.

   ...months. All surveys were anonymous to encourage robust and confidential self-assessment and feedback. ...

7. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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   Blog Post

   Published

   Dec. 15, 2016, 3:57 p.m.

   ...this, mini-λ can readily be purified from bacteria just like a plasmid. Phage A final option for expressing...

8. Plasmids 101: Using Transposons in the Lab

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   Blog Post

   Published

   July 6, 2021, 1:28 p.m.

   ...eukaryotic and prokaryotic genomes. They require just two key components, the transposon’s DNA sequence...

9. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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   Blog Post

   Published

   Aug. 15, 2017, 1:24 p.m.

   ...germinal center of murine lymph nodes. However, one must pay attention to time scale when using photoactivatable...

10. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post

    Published

    May 14, 2024, 1:15 p.m.

    ...more MoClo? Check out Addgene's MoClo Guide! Customizing MoClo for your needs As you probably realize ...

11. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

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    Blog Post

    Published

    March 21, 2024, 1:15 p.m.

    ...compartments in in vivo model systems, so extra care must be taken to assess if proper staining is achieved...

12. How to Deposit Your Plasmids with Addgene

    Type

    Blog Post

    Published

    Feb. 27, 2024, 2:15 p.m.

    ...from a few hundred to millions. Pooled libraries must be verified by next-generation sequencing. Please...

13. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type

    Blog Post

    Published

    Jan. 13, 2025, 9:49 p.m.

    ...edited strand as the template. The PE3 system does just this by including an additional sgRNA. Using this...

14. Technical Design of a Western Blot

    Type

    Blog Post

    Published

    Aug. 22, 2024, 1:15 p.m.

    ... with? It can be so overwhelming that you might just stick with the protocol your labmate handed you ...