We narrowed to 904 results for: CAN

...yours and your team's—can lead to frustration. Clinging to technical tasksDelegation can feel unnatural for...contemplating the leap into management, the journey can be as exciting as it is challenging. How do you... you need to know.   Figure 1: Management can seem a mystery at first! Image used under CC.  ...hands-on work. The temptation to "do it yourself" can result in burnout and underutilized team potential...connecting with mentors who have made similar transitions can provide you with insights, lessons from their experiences... Myers-Briggs Type Indicator or DISC assessments can help you navigate team dynamics effectively and foster... The Making of a Manager by Julie Zhuo Radical Candor by Kim Scott Courses: Leadership programs on...

...metabolism of leaves can be changed by light and temperature and leaf autofluorescence can interfere with microscopy...that replicate current knowledge, but the sensor can also kinetically outperform current methods. Unger...nucleotide polymorphisms present in the human population can affect drug and dose response. Selective serotonin...Unger ultimately hopes that medical professionals can genotype the patient and rapidly test the variants...promoters responsive to water or nutrient levels can help him determine whether the artificial leaf behaves...labelled with a constitutively fluorescent reporter can also be used to track how one population responds...Mitja Remus-Emsermann’s lab at the University of Canterbury where he studies how bacteria interact with each...

...Gal4/UAS system, and you can read more about them in review articles (2–4). You can also take a look at my...expressed when Gal4 is expressed. Gal4 expression can be regulated by any chosen promoter, and Bloomington...expressing Gal4 in your tissue type of interest. You can quickly mate your UAS-YFG line with a variety of ... next blog post). For example, the hedgehog gene can be overexpressed in several cell types by using different...Controlling multiple genes with Gal4/UAS system One can also use Gal4 to drive expression of multiple genes...’s not always clear which side he roots for. You can try to convince him to use Twitter @jonchowphd but...

...grants and projects that might help your work Tenure can depend on your national recognition and the more ...academia is all driven by personal connections What can you do to get the most from the time and money you...conference. Large conferences have many opportunities but can be overwhelming as far as meeting new people. At ... want to introduce yourself and what you do. You can probably use some scientific terms when socializing... that already). Start with, “Is this seat taken? Can I join you?” Next talk about the food and then move...up to someone much more senior than you, but you can safely seek out scientists your own age. Some will...LinkedIn users notice this sort of connection. You can also see and offer congratulations on job changes...

...Figure 1). Antibodies can be broken up into smaller parts, the variable regions can be altered for specific...If you think functionality can be ramped up even more, the best mutant can then be used as the starting...delivering other treatments, their efficacy can be significantly increased through structural and functional...specific functions, the constant regions can be altered for different isotypes, and so much more. How you...low-fidelity polymerase, like Taq. Other PCR conditions can be changed to further lower fidelity, like changing... and the dNTP ratio. The resulting random errors can give you new and unexpected sequences. Degenerate...mutate whole antibody sequences, degenerate primers can be designed to selectively mutate specific regions...

... determines which tissues and cells an AAV can infect) can influence an AAV’s value greatly. Applying ...have different tropisms but, as you can see, AAVs of each serotype can transduce multiple tissues. So how...transducing different brain regions. Although AAV2 can transduce many tissue types, it has low expression... It has been demonstrated that certain serotypes can undergo axoplasmic transport. These AAVs may move... If axonal transport is not desirable, transport can be reduced by injecting a smaller dose of AAV and... than other viruses, multiple freeze thaw cycles can still reduce AAV titer. For long term storage, make... begins. ScAAV avoid this rate limiting step and can immediately undergo replication and transcription...

...which level of purity. Based on these premises we can classify a plasmid preparation in 3 different ways...electrocompetent cells is minimal (picogram range). It can be extracted from small bacterial cultures (i.e. ...prokaryotes or eukaryotes into a synthetic vector that can have a plasmid or viral origin. The type of cloning... extraction. Restriction enzyme based approaches can require several hundreds of nanograms or micrograms...you want to utilize your plasmid DNA, so that you can decide what will be the optimal type of plasmid preparation...

...Career and skills development can happen anywhere, whether you realize it or not. In the lab, you could...two projects: one “safe” project that you know you can get a publication with and one “shoot for the moon...you don’t think you’re qualified. The worst that can happen is they say no, which is infinitely better...Senior Software Engineer “Searching for a new job can be an imposing endeavor.  Break the process down ...Get the toughest steps out of the way early if you can.” - Eddy Page, Digital Media Production Specialist...Kamens, Executive Director Career exploration “You can't wait for opportunities to fall in your lap. Sometimes...

...constructs can be achieved by fluorescence-activated cell sorting, and stable gRNA expression can be achieved... But other enzymatic fusions, such as dCas9-TET, can, like the iron ball-and-chain that they resemble,...site is not as straightforward as expected. TET1 can have many effects independent of dCas9 targeting,...ve got some good news: complete DNA demethylation can be achieved by dCas9 alone, without TET (or any demethylase...demethylase) fusion. This may seem counterintuitive: how can a DNA-binding protein with no enzymatic activity ...DNA demethylation! With optimization, this method can produce nearly 100% demethylation without the confounds...block that unmethylated CpG so that nothing else can interact with it. Remember, the goal of this experiment...

...hybridoma cell lines can produce more than one mAb (Bradbury et al., 2018). Hybridomas can lose gene expression...into a fully functional antibody. These antibodies can be used just as you would use antibodies made from...purified antibodies from a patient’s blood), scientists can use mass spectrometry to determine the amino acid...from an established hybridoma line, the antibody can be converted into recombinant form through DNA sequencing...2010; Andrews et al., 2019). Finally, scientists can perform the entire process of antibody selection ...been cloned into an expression plasmid, the plasmid can be introduced into host cells, such as bacterial,...engineer them. Given the ease with which scientists can now improve genetically-encoded research tools, it...