...workshops and organizational skills. Meanwhile, Addgene was also thinking about ways to address irreproducibility... to Teytelman’s connection with Joanne Kamens, Addgene also had the opportunity to contribute to this ...being adopted by a wider group of researchers. Generating material to empower others to practice and teach...anyone could present the material. For example, Addgene’s contributions by Tyler Ford included reagent sharing...Tsang for editing. Additional resources on the Addgene blog: Check out this guide on Transferable Skills...workshops Levelling Up: Reproducibility for Everyone Addgene: a global resource working to enhance scientific...

...placed on the Addgene website, it sets off a chain reaction of events throughout all of Addgene - within the... of thousands of plasmids a day, like we do at Addgene, pen and paper will fail you. The benefits of lab...fun too. Kris Shamloo is a Software Engineer at Addgene and is interested in writing software tools to ...krisshamloo.com     Additional Resources on the Addgene Blog Learn about How We Use Barcodes in the Lab...Track Your Experiments Additional Resources on Addgene.org Find Plasmids for Your Research ...

...interaction.  In as little was one minute BASU generated a strong streptavidin signal (~250-fold enrichment...point, but one minute of labeling with BASU still generated a >30-fold enrichment compared to E. coli’s BirA...host protein critical to Zika replication. Human genetic disease. RaPID can also be used to test how RNA... answer? The RaPID plasmids are available from Addgene! References Ramanathan, M., Majzoub, K., Rao, ...PMCID: PMC4054858.   Additional Resources on the Addgene Blog Study Protein-DNA Interactions with CUT&RUN...resources for studying chromatin Resources on Addgene.org Find Split-BioID plasmids Find CRISPR plasmids...

... Resources on Addgene.org Unconjugated antibodies at AddgeneAntibody plasmids at Addgene... functionality of the conjugate. For example, Addgene’s antibodies are provided in a buffer with the antimicrobial... Schibli R (2015) Antibody Conjugates: From Heterogeneous Populations to Defined Reagents. Antibodies ...10.1038/nmeth.4380  Additional resources on the Addgene blog Antibodies 101: Buffers, Storage, and ConjugatesAntibodies...

... Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene  ...antibodies work?  Secondary antibodies are antibodies generated against antibody isotypes from a specific species...classes, it is possible to get secondary antibodies generated against isotype subclasses, such as mouse IgG2...my goat anti-rabbit?!”*  *a secondary antibody generated in goat against rabbit immunoglobulins. References...References and Resources More resources on Addgene’s blog Antibodies 101: Introduction to Immunofluorescence...

...interesting discoveries. You’ve analyzed the data and generated beautiful figures. And, you can’t wait to tell...need to first make your poster. While a poster generally contains the same sections as a primary research...references and contact information, and it is generally acceptable for the font here to be much smaller...it’s good to let your ingenuity shine, and you generally have free reign over how you put your poster together... as rows. It can be more catchy because it is generally less common and will stand out; however, be careful...make it your own! Additional resources on the Addgene blog Check out this blog post on navigating the...

...monoclonal antibodies; Addgene collaborates with these companies to create and provide general availability of...recombinant forms (rAbs). mAbs and rAbs have higher homogeneity than polyclonal antibodies because of their immunoglobin...that can be produced in large “copies.” This homogeneity circumvents the batch-to-batch variability found...that the primary antibody is very weak or with general non-specific binding. If the sample differs from...10.1371/journal.pbio.1002165 More resources on the Addgene blog Antibodies 101: ValidationSelecting the Right...

...GFP/EGFP) for a transcriptional reporter or as a general cytoplasmic label of cells, there's no problem.... of the FP. The FP is confined to a plane and generally only rotates in 360°, increasing the probability...can be monomerized with an A206K mutation [2]. Addgene has many of these constructs and they are designated...PMCID: PMC3324619. Additional Resources on the Addgene Blog Read Professor Snapp's Post on mOX FPs Find...in Your Experiments Additional Resources on Addgene.org Browse our Fluorescent Protein Resources Find...Plasmids that Glow (Luciferase Plasmids) Find Optogenetics Resources ...

...skill set Resources on the Addgene website Become of a member of the Addgene depositor community ...a conference, be sure to practice beforehand. Generally, two types of people will visit your poster: those...the tweep up (and for the entire conference, in general). After the conference Review your favorite conference... considered networking, but here are some more general tips. Before the conference Networking is key even...your scientific conference? Resources on the Addgene blog Read other posts on scientific sharing, scientific...

...animation is becoming a TikTok series! Follow @addgene on TikTok for bite-sized portions of antibody concepts...an antibody?" or "what is an IgE isotype?"  @addgene Ready to learn about antibodies? Our new antibodies...molecularbiology #science #scicomm ♬ original sound - Addgene We'll be posting these animations regularly ... all of our videos will also be shared on our @addgene Instagram account, or you can watch the full animation...on our YouTube channel.  More resources on the Addgene blog Antibodies 101: Introduction to Antibodies...

...program are here. This was a great opportunity for Addgene to present our own data on plasmid deposits and...and distirbution for these fast moving fields.  Addgene is a global nonprofit plasmid repository. Over ...Over 2,000 labs have deposited plasmids to Addgene and we distribute over 130,000 plasmids in 2014. Thus,...combination of open-source distributors such as Addgene, as well as a number of online user forums such...Meeting Eric presented a poster with data comparing Addgene global deposit and distribution trends for Zinc...

...open access journals.   Check Out Addgene Videos Over at the Addgene Youtube Channel   After watching ...what online tools people generally like to use to share ideas – with the general goal of seeing if and how...Communication 2.2 (2014): 2. Additional Resources on the Addgene Blog 6 Tools & Tips: Online Social Networking ...Sharing in Research Publications Resources at Addgene.org Check out our Protocol Pages for Video Protocols...

...Addgene Executive director, Joanne Kamens, recently participated in a Reddit AMA (short for “Ask Me Anything... sales-y in posts which falls in line with our general mission to share- as opposed to sell- science. ...serious career dilemmas. Some questions were about general advice on how to meet people, how to transition...content on many of the requested topics to the Addgene blog The blog has many other excellent posts on...and happy to help. Additional Resources on the Addgene Blog: 10 Ways to Share Your Science 6 Tools & ...How We're Sharing Science through Video with the Addgene Youtube Channel Check Out Vidoes of Gender & Science...

...pharmacology (many of which are represented in Addgene's Special Collections). Arguably, oncology has benefited...examining whether the cell lines had isoenzymes and genetic polymorphisms specific to their origins. This technique...out by the commercial repository. Repositories generally ensure cell line identity and purity making it...to the original as possible and so any results generated are reproducible and reliable. When you order ...Abcam Cell Culture Guidelines Resources on the Addgene Blog Learn All about Mammalian Vectors Read up...Using GFP for Protein Localization Resources on Addgene.org Find Plasmids for Mammalian Cells ...

...work is done in flow cytometers, and output is generated through measurement of events. An event is something... machine is plotted as a dot on an X,Y axis to generate a dot plot (Figure 1). Clustered events are called...                         Figure 1: While the plot generated often looks like the graph on the left, it might...intersection of X,Y. For flow plots, however, that is generally not true. Because of the variations in fluorescent...Of course, this is quite easy to do on a plot generated in Biorender! While a helpful visualization tool...10.1371/journal.pone.0137550 More resources on the Addgene blog Conventional vs. Flow Cytometry Plasmid-based...

...resources on the Addgene blog: Introduction to Immunofluorescence View all of Addgene's antibody posts  ...Resources on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene  ...that interact with nucleic acids. Labs studying epigenetics routinely use the chromatin IP (ChIP) technique... found this blog helpful be sure to check out Addgene’s blog for other antibody topics.     References...

...engineered ascorbate peroxidase (APEX), used as a genetic tag for electron microscopy, could be used for ...(Che and Khavari, 2017). Find APEX plasmids at Addgene! Since the development of BioID and APEX, optimized...These newer tools are also currently available at Addgene.  BioID2: A smaller improved promiscuous biotin...utilized yeast display-based directed evolution to generate two mutants, the 35 kD TurboID, which has 15 mutations...et al. "Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy." Nature...PMCID: PMC5467174. Additional resources on the Addgene blog Plasmid 101 Protein Expression Plasmid 101...

...Science Communication Resources at Addgene.org Check out Careers at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...small tasks (like... preparing plasmids to send to Addgene). Following my advice from above, you should probably...appropriately. Back in my undergrad days, I had general goals for my experiments, but didn’t spend enough... in the comments! Additional Resources on the Addgene Blog Management for Scientists eBook Get Advice...

...partnered with Addgene to make our collection of integrin antibodies available through Addgene’s repository...10.1038/s41467-022-35173-w More resources from Addgene Addgene's Antibody Guide Antibodies 101: Flow Cytometry...development, extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...on these cells. What’s next IPI is working with Addgene to accelerate biomedical research by distributing...

...remotely instead of from the lab. Nonetheless, Addgene’s annual Deck the Lab competition drew upon the ...Decoration Competition! #ScienceFun #ZoomParty @Addgene pic.twitter.com/drNBUdlQco — The Tarling-Vallim...CVRHutchinson #SciArt #STEAM #WinterWonderlab @Addgene Make your own here!: https://t.co/AnltWfKuQg pic.twitter.com...description of all the ornaments. #DeckTheLab @Addgene pic.twitter.com/GsAlD6sjM6 — Dr. Anne A Madden ...thank you to everyone who entered. Keep an eye on Addgene’s social media throughout the year for more fun ...