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We narrowed to 898 results for: CAN

Showing: 701 - 720 of 898 results

  1. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ... kb in length. Human adenovirus type 5 (hAdV-C5) can be considered the prototype, although many viruses...available by this deletion from the genomic plasmid can be used to house a recombinant sequence (transgene...potentially because some of the wild-type viruses can persist as a latent infection in certain tissue reservoirs...using vectors, and immune inactivation of the vector can still occur even in the case of no previous immunity...basic research, like other viral vectors, though significant challenges remain (Wold & Toth, 2013).  While...Adenovirus Vectors for Gene Therapy, Vaccination and Cancer Gene Therapy. Curr Gene Ther, 13(6), 421–433. https...

  2. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    Published
    Feb. 23, 2023, 2:15 p.m.
    ...research applications. Scientists around the world can upload AAV validation data, sharing what works and...community to maximize the positive impact that science can have on the world. ...

  3. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    Published
    Sept. 1, 2016, 2:30 p.m.
    ... graduate student life. Senior graduate students can sometimes be elusive or have a certain mystique that...our next episode, Niroshi and Ben discuss how you can leverage your graduate school community to help keep...beyond graduate school. Ben J. Vincent is a Ph.D candidate in the Program in Genetics and Genomics at Harvard...

  4. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...titer as compared to previous lentiviral vectors. It can be used both to knock-out single genes in clonal ...need help cloning a gRNA into lentiCRISPR v2, you can take a look at the protocol kindly provided by the...used for includes work probing mTOR signaling in cancer cells, deciphering the mechanism of matrix metalloproteinase...

  5. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ... toolbox. As we’ve covered in Plasmids 101, tags can help you determine localization of a protein of interest... tags? Short epitope tags like FLAG, HA, and Myc can adopt a number of different conformations and thus...antibody. From their initial pool of candidates, they selected 12 candidate protein domains from non-vertebrate...

  6. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...world by storm. Like RNA interference, these enzymes can knock down RNA without altering the genome, but Cas13s..., the Cas13d orthologs described in these papers can independently process their own CRISPR arrays into... require a protospacer flanking sequence, so you can target virtually any RNA sequence! In bacteria, Cas13d-mediated...efficiency; where shRNA treatment produced 500-900 significant off-targets, CasRx displayed zero. Unlike Cas9...

  7. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...more concurrent projects in varying stages, which can get messy if you’re not on top of it. In a science-based...yourself with knowledge of software development so you can pick up the jargon and know the concepts as soon ... technology tools with the customer in mind. You can follow her on twitter @rgrubinstein. Additional resources...teams that run within the agile framework If you can’t find internships at science companies, try to find...themes and customer pain points we’ve identified. I can’t wait to send it out. 4:00 PM--Respond to customer...

  8. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...components are used to package lab grown viruses. It can be intimidating at first to look at so many plasmid...). VSVG is a broad tropism envelope protein that can also be pseudotyped to alter infectivity. Next, the...While not required for lentiviral production, they can be helpful for certain applications and aid in ease...

  9. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...serum There are a few different ways the antibodies can be isolated from the serum. Protein A/G purification... the immunogen. A different type of purification can be performed to isolate antibodies only recognizing...different epitopes of the target protein. While this can increase background staining (see next section), ...

  10. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ...change rapidly during neuronal activity, scientists can use GECIs to watch neurons fire under the microscope... multicolor experiments. With OCaMP, researchers can image multiple brain regions or cell types at once...The Lohman lab has a limited quantity of AAVs that can be requested by contacting Alexander Lohman (alex.lohman...

  11. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ... sex of their cells when doing experiments—which can lead to failed research. For example, an international...cells and cell lines degenerate over time—and sex can be hard to determine. To remedy this, Shah et al.... been made in this area in recent years, and you can see a summary of this literature in our health and...of cardiovascular disease, 126,000 fewer breast cancers, and 145,000 more quality-adjusted life years. ...Shah et al., “Do you know the sex of your cell?” I can’t tell you how many researchers I’ve talked to who...the most commonly used cell lines appearing in American Journal of Physiology-Cell Physiology over the...product design. But eyes have been opened—and we cannot return to a world that ignores gender. Many thanks...

  12. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...for a curated list of coregulators). Coregulators can function as coactivators and corepressors depending...complexes, a member of a single coactivator complex can serve as a rate-limiting conduit to control the actions...and reagents in the field. Additional information can be found in the Datasets and Reagents sections of...of therapeutic indices, including hypertension, cancer, diabetes, cardiovascular disease, cholesterol ...

  13. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ... template to Cas9 Homology-directed repair (HDR) can be used to make precise modification in genome editing...every 8 bp). Nme2Cas9 is also small enough that it can be packaged into AAV. The lab used single-AAV delivery...base editors Nme2Cas9 CRISPR interference in Candida albicans E. coli genome-wide inhibition library To...All-in-One AAV constructs CRISPR interference in Candida albicans A new CRISPR interference (CRIPSRi) platform...platform for targeted gene repression in Candida albicans was developed by Rebecca Shapiro’s lab. After testing...libraries also include 2,400 gRNAs without any significant hit across the E. coli genome as a negative control... fold and >3,800 fold in human cells with no significant reduction in editing efficiency. Read the paper...

  14. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ...Anyone affiliated with the recipient organization can create an Addgene account and place an order: a purchasing... How do I pay by purchase order (PO) number? You can find answers to other ordering questions here. In...it on behalf of depositing institutions. Addgene cannot fulfill orders based on PO documents that are sent...

  15. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ... features we can offer online for Addgenies and to serve external customers. I also can’t wait to see ...DNA distribution to ensure scientists everywhere can access plasmids despite the evolving export/import...one of the main reasons I have so few worries and can often focus on the excitement of what we are accomplishing... employee pool and we get a lot of successful candidates from internal referrals. Our long reaching reputation...

  16. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ...and enable scientists to discover if known drugs can be used to treat these diseases (drug repositioning... found that low concentrations (≤3 mM) of GdnHCl can functionally rescue multiple Arg-mutant proteins ... developmental delay. Mutations in NFIA and NFIB can also cause developmental delay in patients. We have...physiological and pathological roles of human cell glycans. Accordingly, she joined efforts with the Portuguese...They result from defects in different steps in the glycan biosynthetic pathway and have a wide range of clinical...which leads to nearly 25% child mortality. Since glycans play an essential role in almost every aspect of...NFIX gene and test whether variants of unknown significance (VUSs) that are frequently found in genetic ...

  17. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ... complicated solution like cell lysate. But they can be a lot of work — and what if you want to detect... stripping is here! Rather than start over, you can remove the detection antibodies from your membrane...brittle, so proceed with caution. Unlike PVDF, they can be returned directly to buffer without a re-wetting...

  18. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ...susceptible to redox modification, the MPST hydrosulfide can be transferred to the roGFP2 cysteine thiols and ...change its fluorescence properties. The biosensor can also be used along other thiol-containing acceptors...further enrichment. Tian notes that this platform can be used to engineer many types of biosensors.   This...the brain. “We hope that iGluSnFR3 will enable significant steps forward in our understanding of synaptic... Brandán Pedre Pérez is a postdoc at the German Cancer Research Center (DKFZ) who has developed new redox...withdrawal Elizabeth Hubbard is a fourth year Ph.D. candidate at the University of California, Irvine where ...

  19. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...I ended up at pgEd, what I do there, and how you can set yourself on a path towards a similar position...turn crucial if we want to make sure that everyone can be informed and empowered to make their own decisions...ability to talk about science in a language that can be understood by those who are not experts in particular...

  20. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ... to thymine Since an enzyme that excises inosine can be modified to target guanosine instead, perhaps ...editors, called gTBE and gCBE, with Addgene. gTBE can introduce T → C and T → G edits, though with limited... CGBEs. This distinct approach means researchers can now select the best tool for their application from...options.  Conclusion With these advances, every base can now be directly targeted by a base editor, although... accurate, more efficient, and more diverse. We can’t wait to see what tools the field will come up with...
Showing: 701 - 720 of 898 results