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Addgene

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We narrowed to 846 results for: ELL

Showing: 701 - 720 of 846 results

  1. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...pathways that are frequently implicated in cancer cell proliferation, survival, differentiation, and apoptosis...pathway. “The idea is that if you introduce them into cells one at a time, you could identify those that confer...

  2. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...insertion. The linear PCR product is introduced into the cell by direct transformation. A given insert will typically...protein-protein interactions via FRET. Sidae Lee, Wendell Lim, and Kurt Thorn at UCSF have recently developed...

  3. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...upload and share their negative data. After all, they tell me, when something doesn’t work, it’s not a failure...approach for deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the National ...

  4. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...small Cas protein, is active in E. coli and human cells Two years ago, Jennifer Doudna’s lab discovered ...demonstrating that CasX is active in both E. coli and human cells. Using the structure of CasX at eight different ...

  6. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ..., and VP3, which together form the outer capsid shell that protects the viral genome. The cap gene is ...for serotype-specific differences in tissue and/or cell type tropism. Introducing changes and/or short peptides...

  9. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ... region of a different species and/or isotype (Liddell 2013). In most cases, the resulting chimeric antibody...antibodies! References and resources References Liddell, E. (2013). Antibodies. In The Immunoassay Handbook...

  13. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ... or more “important”, they just need to know you well enough to ask good questions and hold you accountable... can share it with others and get their input as well. Giving a great video presentation is going to be...

  14. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...industry?” Once academia was the clear answer with a well-defined path to professorship. But recently a downward... professions increases your chances of finding a well-suited job. EAs shouldn't be viewed as an unproductive...

  15. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...allow scientists to express a gene of interest in a cell or animal model. AAV plasmids contain several important...Integrity on rAAV8 Production Using the Baculovirus/Sf9 Cells System. Hum Gene Ther Methods, 28(5), 277–289. https...

  16. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...hard-to-read sentences.  Grammarly Grammarly is a lot like spell check, but since it’s an internet browser plug-in...managers and productivity tools for grad school Hello PhD Podcast episode 15 "Simple tricks for time management...

  17. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...again or set up a meeting. If you really got along well, suggest a coffee chat in the near future. Even ...you succeed in any way. Don’t believe people who tell you that thank you isn’t necessary. I once got a...

  18. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...Addgene deposits? Even more interestingly, could we tell if depositing a plasmid with Addgene causes a paper... most relevant to plasmids: Genetics & Heredity, Cell Biology, Biotechnology & Applied Microbiology, Biochemistry...

  19. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...skip purification steps and end-repair steps with well-designed fragment overlap sections, while still ...of NEBuilder HiFi DNA assembly method As with any well-executed cloning endeavor, NEBuilder requires upfront...

  20. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...measure the pH of our samples, we used a solution of cell culture medium, which is buffered and contains the... 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer P, Manning MC, Fitchmun MI. Protein...
Showing: 701 - 720 of 846 results