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...intern who shared their experience working at Addgene. An Addgene Summer Internship Matteo Tardelli, who ...contributing to the Addgene blog? We'd love to hear from you! Email us anytime at news@addgene.org. ... very thankful time of year for those of us at Addgene! And we can’t think of any better way to start ...to the blog. Thank you! More resources on the Addgene blog 10 Great Guest Posts We're Thankful For Lucky...

...molecular weight ladders were recently deposited with Addgene. A team of undergraduate students led by Dr. Song...restriction digested with PstI or EcoRV, these plasmids generate 100 bp or 1 kb DNA ladders, respectively. Unlike...pPSU1 and the pPSU2 plasmids are available from Addgene. Learn How to Analyze a Restriction Digest References.... L., & Tan, S. (2017). The pPSU Plasmids for Generating DNA Molecular Weight Markers. Scientific Reports...Central PMCID: 5446395. Additional Resources on the Addgene Blog Are you a Frugal Scientist? Save Money with...the Evolution of Lab Techniques Resources on Addgene.org Use the Kit-Free Alkaline Lysis Plasmid Miniprep...

...production requires animal sacrifice and precludes genetic engineering of a tag or fusion with a reporter ...described in Pleiner et al, the key steps for generating nanobodies are outlined below; no alpaca required...your work. Species Antibody Subclass Nanobody Addgene Plasmid Rabbit N/A* TP897 pTP1183 (1x cysteine...nanobodies from the column. bdNEDP1 protease can be generated using plasmid pDG02583. Label the Nanobodies: ...29263082 Find plasmids from this publication at Addgene. 2. Bates, M., Böhning, M.I., Chug, H., Görlich...PubMed PMID: 23495938. Additional Resources on the Addgene Blog DIY DNA ladders from Penn State University... protein expression Additional Resources on Addgene.org Find more nanobody expression plasmids here ...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Plasmid Rap Plasmid rap video made with support from Addgene.      Check out more science education tools...looking for creative ways to implement the next generation science standards and communicate science. Learn...Additional Resources on the Addgene Blog Get Advice on How to Build a Grad Student Community Check out All...Plasmids 101 Series Additional Resources on Addgene.org Check out Our Protocol Pages for New Protocol...

... Sign up to write for the Addgene blog! To sign up to write for the Addgene blog simply email us. We can...write for us! Click Here to Contribute to the Addgene Blog Possible guest topics Some topics you might...Exposure - With over 100,000 views each month, the Addgene Blog is a great way to get your name out there!...include on CVs and Resumes. An Expanded Network - Addgene will promote your blog on a variety of social media...applying CRISPR for mouse genome editing. Post: Generating Mouse Models Using CRISPR/Cas9   Erik L. Snapp...

...our best guess). Follow @Addgene, use the hashtag #DeckTheLab, and tag @Addgene in the post! In January...It’s finally December, and here at Addgene HQ in Massachusetts that means it’s time for near-freezing...winner, you’ll receive a winter care package from Addgene.  Here are the details:   Contest 1: Deck the ...CVRHutchinson #SciArt #STEAM #WinterWonderlab @Addgene Make your own here!: https://t.co/AnltWfKuQg pic.twitter.com...however you celebrate! And if you want to see how Addgene celebrates and decorates, follow us on Twitter ...

...experiments, let us know (datahub@addgene.org) and we might just send you a new Addgene Data Hub t-shirt!      ... out the redesigned, revamped, and re-launched Addgene AAV Data Hub, the place for scientists to share...curated collection of community submissions for Addgene and CLOVER AAV experiments - and we've updated ...Submitting to the Data Hub If you've ever validated an Addgene or CLOVER AAV, you can (and dare we say, should...step-by-step submission guide or email datahub@addgene.org to learn more. A great submission includes: ...

...quietly learning about Addgene’s past and present, but the real promise of Addgene lies in our future. As...Addgene saw big changes in 2022, from the launch of a new material to the redesign of a community resource...collection, which includes both neuroscience and general-use antibodies, is available in both trial- and...organization. One thing that will remain the same is Addgene’s commitment to supporting the research community...number of people. I look forward to working with Addgene colleagues, collaborators, partners, and the larger...

...accessible to the general public both in terms of cost and content level. Sponsorship from Addgene and Harvard... Here at Addgene, we’re dedicated to advancing and sharing science! In association with the Harvard graduate...DayCon. DayCon is a one-day conference aimed at the general public that provides an accessible introduction...introductory lectures targeting three broad concepts: the genetic basis of disease, cancer, and viral infections.... do you share your science with the community? Addgene would love to hear about your efforts to engage...

... Biology Resources on the Addgene Blog Deposit your plasmids with Addgene and make your work more accessible...copes with the growing amounts of digital data generated in labs. While many labs approach the situation...are available on the market today, ranging from general note taking tools, to more scientific field-based...One comment reflected the desire for a flexible generic solution, rather than an ELN designed for a specific...From pictures to excel sheets, researchers today generate vast amounts of digital data during their work...Twitter @scinoteELN Additional Resources on the Addgene Blog Learn about more tools for reproducibility...

...Are the shots clear enough for the audience? As Addgene’s Science Video Production Specialist, I’m constantly...March 2020, just four months after I started with Addgene, I was about to have a check-in with my boss Jennifer...Cycle 3: A Return to HQ Figure 3: Even Blugene is practicing social distancing and wearing their...traditional solution was completely out of reach for Addgene.  So, onto research! I needed to design a system... needed to figure out a microphone situation. Generally, I record into an external recorder using a lav...the Lab Design: The lab is the beating heart of Addgene. It’s why we exist, storing plasmids, and distributing... out all of the written and video protocols at Addgene? If you need help in the lab, we have you covered...

...Addgene recently attended and sponsored the Future of Research (FOR) Boston Symposium 2015. I personally...fun and surprisingly exhausting experience. As Addgene believes many of the topics discussed during the...are many companies and organizations (including Addgene), that need people who are data oriented, scientifically...skills beyond those required at the lab bench. At Addgene we try to do our part in boosting awareness of ...write for a university publication, improving your general writing skills will help you in your academic publishing...problems within the research environment itself. At Addgene we hope that all parties involved in making academic...Publications of Interest Can Be Found Here Resources at Addgene  Read David Riglar's FOR Boston 2015 Blog Post...

... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR... optimized for Burkholderia, they identified 67 genera of Proteobacteria that this toolkit might be used...TadA) to a catalytically impaired Streptococcus pyogenes Cas9. They called this editor Target-ACE (adenine...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...more information. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

...thread ⬇️⬇️⬇️ — Addgene (@Addgene) April 15, 2020   Additional resources on the Addgene blog Read about...essential research into COVID-19 (like here at Addgene!), and new databases are supporting their access...” said Guillaume Bourque, a Professor in Human Genetics at McGill University. “There is a need to pool...guide for external collaborations Resources on Addgene.org Browse our COVID-19 plasmid collection ...

...people and society. Future generations of IMP Motivating future generations is key to the success of this...from international financial struggle, our new generations feel thrown, to sink or swim on their own, into...knowledge, experience, and new professional capacity generated during this process result directly in positive... growing demand from universities and future generations of professionals has made possible a significant...School, where she studies age-related macular degeneration. She is also a blogger who writes posts for ...Advancemnet of Higher Education website Resources at Addgene Read Other Career Blog Posts Read the Networking...

...sometimes unclear or missed altogether. Emailing Addgene might not be as high on the published-paper-celebration...in this status — a win-win situation for all. Addgene will release plasmids on hold if they are clearly...celebrate!), send us a quick email at deposit@addgene.org with the citation and your deposit or plasmid...distribute unpublished materials. What about preprints? Addgene supports preprints and encourages authors to share... for reading, and for depositing plasmids with Addgene! We'll continue to review our policies to make ...website, Help Center, or email us at deposit@addgene.org. ...

...Additional Resources on Addgene.org Brush Up on Your CRISPR Background with Addgene’s CRISPR Guide Page Find... heat loving) bacteria, such as Streptococcus pyogene’s SpCas9. These Cas9 proteins don’t work well at...try, as always, the plasmids are available from Addgene. Happy CRISPRing! References Harrington, Lucas...bioRxiv (2017): 138867. Additional Resources on the Addgene Blog Read about CRISPR Methods for Bacterial Genome... Find CRISPR plasmids at Addgene ...

...about pseudotyping Resources on Addgene.org Find plasmids in Addgene's COVID-19 collection Find SARS-COV...coronavirus and SARS-CoV. These two viruses are quite genetically distinct from SARS-CoV-2 and its variants. Yet...Balazs lab was quick to deposit their plasmids at Addgene to accelerate ongoing COVID-19 research. “I hope...j.cell.2021.03.013 Additional resources on the Addgene blog Browse all COVID-19 related blog posts Learn...-COV-2 pseudotype virus plasmids from Addgene ...

...language is ... The general representation of data is... The figures are ... In general, I think that this...language in the first (and clunky) version of my general comments and adjust them if my opinion changed ...can be deposited in the specific repositories (Addgene, WormBase, Arabidopsis.info). These repositories...PMCID: PMC4528400. Additional resources on the Addgene blog Find blog posts on scientific sharing Get...tips on writing a review article Resources on Addgene.org Learn about depositing plasmids pre-publication...

...involves amine-reactive biotin derivatives that generate covalent bonds with positively-charged amino acids...solution was to exploit the best of both worlds. We generated a plasmid that produces biotin ligase and a protein...the biotin ligase BirA backbone (Eric Campeau, Addgene plasmid #26624) was unsuccessful. Nothing more ...pMal-T-Avi-His/BirA plasmid now available through Addgene. We have successfully used this plasmid to biotinylate...cell signaling.     Additional Resources on the Addgene Blog Check out other Hot Plasmids Find Additional...Posts on Plasmid Technologies Resources at Addgene.org Learn about Popular Plasmids in the Repository...