We narrowed to 738 results for: HA
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TypeBlog PostPublishedOct. 18, 2018, 12:37 p.m...., TOP10, and Mach1. No common lab cloning strain has failed so far. What is most important is the transformation...
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AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m....well suited for larger transgenes. Unfortunately it has limited tropism in the retina, primarily infecting... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog PostPublishedFeb. 9, 2023, 2:15 p.m.... from R1 institutions to community colleges, and has helped provide original research experiences for ... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog PostPublishedDec. 7, 2020, 2:53 p.m....technology potential enabled by CRISPR-transposons has yet to be fully realized. Many thanks to our guest... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m....expression is sufficient. Lentiviral vector technology has come a long way since the 1990s, both in terms of... -
CRISPR Antimicrobials
TypeBlog PostPublishedMay 3, 2016, 2:30 p.m....caveat that phages are very diverse, and each phage has a narrow bacterial host range. To further potential... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog PostPublishedMarch 17, 2016, 2:30 p.m....measles, mumps, and rabies. Research on Ebola virus has been limited, in part, due to the necessity for working... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog PostPublishedOct. 27, 2015, 3 p.m....enabled numerous cell biology assays. However, there has been a pervasive view that most FPs in a cell will... -
Plasmids 101: Control Plasmids
TypeBlog PostPublishedApril 9, 2015, 3:29 p.m....transfection reagents or the transfection process itself has any cytotoxic effects on the target cells. Another... -
Negotiating Work and Life: How to Find the Joy
TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m....sometimes put work ahead of life Sometimes, work has to come first. That means you and/or your spouse ... -
CRISPR 101: Validating Your Genome Edit
TypeBlog PostPublishedNov. 3, 2022, 12:15 p.m....effects, you can consider using a version of Cas9 that has been specifically engineered for reduced off target... -
Filming Science Videos in the Age of Social Distancing
TypeBlog PostPublishedMay 20, 2021, 1:15 p.m....inch wireless monitor. Wireless video technology has gotten very impressive, but due to costs and availability... -
Transferable Skills Guide: External Collaborations
TypeBlog PostPublishedApril 19, 2018, 1:25 p.m....problem or a need for something, usually someone else has realized this too. Begin with informal conversations... -
Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog PostPublishedApril 8, 2025, 1:15 p.m....reports which POI band is stronger. The right gel has exactly the same POI bands as the left gel, but because... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog PostPublishedNov. 21, 2024, 10:47 p.m....Looking forward Over the past twenty years, Addgene has made huge strides in increasing the accessibility... -
10 Steps to a Perfect Science Talk
TypeBlog PostPublishedAug. 23, 2016, 2:30 p.m.... It makes a great point when I then ask “and who has deposited to the repository?” When most or all of... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog PostPublishedJuly 30, 2024, 1:15 p.m....Addgene have worked hard to ensure that the service has grown steadily, helped by a partnership with the ... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog PostPublishedSept. 13, 2018, 1:55 p.m....Bassik lab created a human knockout library that has 10 gRNAs per gene and targets all ~20,500 protein-coding... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog PostPublishedJuly 10, 2018, 1:08 p.m....Product Marketing Manager at sciNote. Today, every lab has its own habits and approaches to record keeping. ... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog PostPublishedSept. 5, 2017, 1:58 p.m....re-cutting the target sequence once the desired edit has been introduced. We found that blocking mutations...