We narrowed to 878 results for: IND
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TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m....these frustrating and often months-long quests to find the right AAV for your system or to successfully...improve our Addgene Data Hub, 76% of respondents indicated they wanted to see more negative data (Figure ... e-book Additional Resources on the Addgene Blog Find and Share AAV Data with Addgene's New AAV Data HubCelebrate...
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Antibodies 101: Producing Recombinant Antibodies
TypeBlog PostPublishedAug. 13, 2024, 1:15 p.m....supernatant containing antibodies. The antibodies bind the immobilized ligands and can be eluted once the...are incubated with the cell suspension. Antibodies bind ligands on the beads and a magnet is used to separate...Recombinant antibodies should be validated to ensure they bind their antigens specifically. Possible applications... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....are also available (Weigel and Glazebrook, 2002). Find plant plasmid resources at Addgene Choosing an ...background growth of non-transformants and make finding true transformants more difficult. Don’t leave...arabidopsis in the classroom Resources on Addgene.org Find plant plasmids Visit our plant resource page Deposit... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog PostPublishedSept. 13, 2018, 1:55 p.m...., they fail to account for the effects of Cas9-induced dsDNA breaks. Are off-target cutting patterns in...hypersensitivity, or are in an enhancer, transcription factor binding site, or a coding region. Targeting these sites...Check out this guide to pooled plasmid libraries Find all of the CRISPR Pooled Libraries currently available... -
Tips for Writing a Good Cover Letter
TypeBlog PostPublishedMay 21, 2024, 1:30 p.m....that! I organized my lab data so well that I can find the results of any experiment I’ve run in under ...format regardless of whether you’re in academia, industry, or nonprofits. Like any other letter, they have...Our Management for Scientists Series Get Tips on Finding Your Perfect Job after Graduation Additional Resources... -
Plasmids 101: Shuttle Vectors
TypeBlog PostPublishedMay 20, 2025, 1:15 p.m....visually select cells in a microscopy image for individual analysis. A shuttle vector designed for this ...plasmids are those that can replicate in multiple kinds of bacteria, including many natural plasmids. By... same molecular language as the tree outside my window! Whatever you use them for, shuttle vectors can... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog PostPublishedNov. 10, 2016, 3:30 p.m....for a bacterial infection. While this post will, indeed, focus on bacteria, it is important to note that...resistance genes from it’s environment (from DNA left behind after another bacterium died for instance). These...Are Used in the Lab Probe Phage Genomes for Host Binding Proteins Learn How to Use Our Microbiology Pages... -
Five Popular Model Organisms, Part 2
TypeBlog PostPublishedSept. 29, 2020, 1:15 p.m....plasmids for use in CRISPR and Tol2 transposase induced transgenesis. Xenopus Xenopus, or frogs, are one...Addgene.org: Browse our plasmid collection page Find more plasmids and resources for plant resources ...Collection Page Read our molecular biology reference Find plasmids for your research ... -
Tips for Getting a Faculty Position
TypeBlog PostPublishedJuly 20, 2023, 1:15 p.m....to email me at [email protected]." You can find his original blog post below! --Ed. Eight years...fundamental to development. I'm interested in how cells find their way to the correct positions. What is the... fit into a department and bring in grants. What kind of colleague will you be? Do you understand what... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m....hydrogen bonds and a base-pair size nearly indistinguishable from a Watson-Crick base pair, are joined ...conditions to be screened per plate. The results indicated that the optimal buffer for Taq DNA Ligase contains...DNA ligase I completely encircles and partially unwinds nicked DNA." nature 432.7016 (2004): 473. PubMed... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog PostPublishedMay 28, 2024, 1:45 p.m....it is also important to know the brightness of individual fluorophores. Table 1 describes the brightness... conjugated to. Since CD45 is a common marker, finding an antibody already conjugated to my fluorophore... In this post, you learned about the principles behind fluorophores and their colors for used in flow ... -
Viral Vectors 101 eBook 2nd Edition
TypeBlog PostPublishedJuly 11, 2024, 1:15 p.m.... for bench scientists everywhere. In it, you can find an introduction to viral vectors and their elements... in a clean and easy-to-navigate design. You can find it, along with other eBooks in our collection, on... -
Hot Plasmids: Bacillus subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!
TypeBlog PostPublishedDec. 11, 2017, 2:02 p.m....production, and CRISPR tools for plants. You can find additional hot plasmids in our quarterly newsletter... and open science: Interview with Darrell Kotton Find additional plant biology blog posts Resources at... -
Addgene's 20th Anniversary Party
TypeBlog PostPublishedJune 11, 2024, 1:15 p.m....and more. It's this diverse group of talented individuals that makes Addgene — well, Addgene. We're so ...delicious food, or playing one of the giant games or indoor mini-golf course. While the main purpose of ... -
Twenty Years of Sharing Science!
TypeBlog PostPublishedJan. 8, 2024, 3:25 p.m....partnered with distributors in China, Korea, Japan, India, and Brazil. We've also created a robust support...us the honor of being part of yours. We hope you find our services helpful in your scientific pursuits... -
The Codon Guide to Synthetic Biology
TypeBlog PostPublishedSept. 14, 2023, 1:15 p.m....synthetic biology can be difficult to find. I hope that many people will find this guide useful as they start... -
Expanded Access to Rabies Plasmids
TypeBlog PostPublishedFeb. 1, 2024, 2:15 p.m....under this application process since May 2020. We find this process takes 4-6 weeks on average. Therefore...licenses is available through the US Bureau of Industry and Security. If you have any questions about ... -
DIY DNA Ladders from Penn State University
TypeBlog PostPublishedJuly 14, 2017, 2:30 p.m....interested in making your own DNA ladder? You can find the Tan Lab’s DNA ladder prep protocol here and ...Frugal Scientist? Save Money with the Baby Gel Box Find 5 Smart Phone Apps for Lab Life Learn About the ... -
New Educational Resource: The Addgene Videos Page
TypeBlog PostPublishedJune 16, 2017, 2 p.m....resource for scientists around the world. With that in mind, we are very excited to debut our brand new Addgene...goal of this page is to make it easier for you to find content relevant to your research and career goals... -
Bringing Renewable Energy to Addgene
TypeBlog PostPublishedAug. 27, 2019, 1:08 p.m....in New England- the majority of which are solar, wind, or hydroelectric in nature. The operation of -80C...other scientists save resources by sharing plasmids Find plasmids at Addgene ...