Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 847 results for: PLE;

Showing: 701 - 720 of 847 results

  1. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...food pantry or community arts organization. Many people are surprised to learn that Addgene is officially...thought leader in scientific sharing, leading by example to increase sharing in the community Addgene fosters...

  2. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ...serendipity during your PhD studies and offer two simple steps to welcome these unforeseen moments. Widening...successfully transitioned to industry after his PhD and a couple of postdocs focusing on molecular biology and metabolism...

  3. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ... publications cited. If the information seems incomplete or unclear, Bairoch follow up via email to the... Multiple Authors...

  4. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...promoter lead to higher transcriptional activation. Multiple nuclear localization signals (NLS) increase protein... However, this system is far from perfect. For example, If your fusion protein is expressed at low levels...

  5. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ... are leaders in the field of microbiology with multiple high-profile publications. My classmates consisted...portion of the paper will be generated. After you complete a publication quality figure, produce the figure...

  7. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ...be successful in a specific role or cycle. For example, if you try to lead when you are expected to be...Got You Here Won't Get You There: How Successful People Become Even More Successful, by Marshall Goldsmith...

  8. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...has the CMV promoter for mammalian expression, a multiple cloning site to insert YGOI, a bacterial selection...e.g. pLKO.1 - TRC cloning vector). Check out our complete guide to empty backbones for additional details...

  10. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...(on a schedule) I hope you all know by now that people get jobs at least 85% of the time because of relationships...pretty easy to make 30 minute appointments with people all over the world. Think about contacting alumni...

  12. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...the public’s perception of head injury risk. In completing a master’s thesis in science communication, I... Department of Science Communication where he completed a master's degree in 2020, his research explores...

  14. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...The technique relies on the basic ability of complementary basepairs adenine (A) and thymine (T) to hybridize...PCR product. If you need to decrease error rate, please use one of these methods to ensure your insert ...

  15. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...efficient in primary fibroblasts. iTOP also works in multiple types of primary mouse cells, including neuronal...and continued protein expression would require multiple transduction events. For these reasons, iTOP is...

  16. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ... out more than necessary for my interview. The simplest place I could think of to start was “Marketing...about marketing would be to start reading a broad simple overview, learn some jargon and key phrases and...

  17. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...(by CRISPRainbow) to 3-fold with CRISPR-Sirius. Triple color detection With a third sgRNA containing alternating...Central PMCID: PMC4364232. Ma, Hanhui et al. “Multiplexed labeling of genomic loci with dCas9 and engineered...

  18. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...point of reference, Addgene commonly prepares NGS samples using both pre- and post-amplification DNA and ...representation is most easily understood visually. As an example, the curves from our recent amplification of the...

  19. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...and transgene expression (Zhou et al. 2017). For example, mutations in the RBE site reduce binding with ...AAV2 ITRs can be cross-packaged with capsids from multiple AAV serotypes (Wilmott et al., 2019), and different...

  20. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...portion of different antibody isotypes. Binding to complement system proteins, which can initiate a cascade... next to a different constant domain gene. For example if a B cell switches to producing IgE antibodies...
Showing: 701 - 720 of 847 results