We narrowed to 855 results for: PLE;
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TypeBlog PostPublishedSept. 10, 2019, 12:56 p.m....arms that flank the donor plasmid cargo are complementary to either side of the double-stranded break ...included in the next What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest...
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New Norepinephrine Indicators: nLightG and nLightR
TypeBlog PostPublishedDec. 12, 2023, 2:15 p.m....GFP), and nLightR uses a red fluorescent protein, mApple. Kagiampaki et al. found nLight is suitable for...both colors allows for increased options for multiplexing in experiments, including ones that study alpha2... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog PostPublishedJuly 9, 2015, 2:30 p.m....are appying for diffrent types of positions (for example, bench research vs. project managment) your resume...a specific department and/or location. The more people you meet the better chance you have of finding ... -
London Calling - Addgene’s New Europe Office in London
TypeBlog PostPublishedJune 10, 2014, 3:20 p.m....microbiology, virology, and genetics - before going on to complete her Masters degree at Imperial College London ...ideas with others. This love of interacting with people and talking about science makes her the perfect... -
Cellosaurus: A Cell Line Information Database
TypeBlog PostPublishedFeb. 21, 2023, 2:15 p.m.... publications cited. If the information seems incomplete or unclear, Bairoch follow up via email to the... Multiple Authors... -
Sensing Neuronal Dopamine
TypeBlog PostPublishedJuly 30, 2019, 12:59 p.m....this frequency can indicate behavioral state, for example, if someone is awake, agitated, or asleep. Though...neurotransmitters in various species and while exhibiting complex behaviors. Find dopamine and other biosensors in... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog PostPublishedJuly 27, 2021, 1:15 p.m....organoid model (Sun, et al. 2021). Figure 1: Example schematic from the Organoid EasyTag system, showing...Resources page. Here are some highlights from the past couple months: A new lentiviral CRISPR library from Alec... -
We're Updating Our Hold for Publication Policy
TypeBlog PostPublishedJune 24, 2025, 1:15 p.m....article is published. This policy gives us time to complete necessary quality control and legal approvals ... your trust, and meet the community’s needs, so please let us know what you think. We appreciate your ... -
Embracing Serendipity: A Crucial Element in the PhD Journey
TypeBlog PostPublishedApril 25, 2024, 1:15 p.m....serendipity during your PhD studies and offer two simple steps to welcome these unforeseen moments. Widening...successfully transitioned to industry after his PhD and a couple of postdocs focusing on molecular biology and metabolism... -
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog PostPublishedJune 5, 2017, 3:19 p.m....food pantry or community arts organization. Many people are surprised to learn that Addgene is officially...thought leader in scientific sharing, leading by example to increase sharing in the community Addgene fosters... -
SunTag and Fluorescent Imaging
TypeBlog PostPublishedMarch 28, 2017, 2:30 p.m....promoter lead to higher transcriptional activation. Multiple nuclear localization signals (NLS) increase protein... However, this system is far from perfect. For example, If your fusion protein is expressed at low levels... -
Tips for Improving Your Next Manuscript
TypeBlog PostPublishedNov. 22, 2016, 3:30 p.m.... are leaders in the field of microbiology with multiple high-profile publications. My classmates consisted...portion of the paper will be generated. After you complete a publication quality figure, produce the figure... -
Networking and Inspiring Young Scientists at Freshers Fairs
TypeBlog PostPublishedNov. 17, 2015, 3:30 p.m....from some Addgene support? Please get in touch, as we aim to support a couple of events a year for the ... -
Management for Scientists: Managing vs. Leading
TypeBlog PostPublishedMarch 31, 2015, 4:08 p.m....be successful in a specific role or cycle. For example, if you try to lead when you are expected to be...Got You Here Won't Get You There: How Successful People Become Even More Successful, by Marshall Goldsmith... -
Choosing Your Perfect Empty Backbone
TypeBlog PostPublishedAug. 19, 2014, 3:39 p.m....has the CMV promoter for mammalian expression, a multiple cloning site to insert YGOI, a bacterial selection...e.g. pLKO.1 - TRC cloning vector). Check out our complete guide to empty backbones for additional details... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....ever like to write about a recent plasmid deposit please sign up here. Human whole genome sgRNA iBAR ...allowed tracing of the performance of each gRNA multiple times within the same experiment by counting both... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....fluorophores that excite in the violet range. For example, mWasabi can be used with Sapphire (excitation ...would like to share with the scientific community, please deposit the plasmids at Addgene! References Ai... -
5 Uncommon Ways to Gain Career Skills During an Uncommon Time
TypeBlog PostPublishedOct. 20, 2020, 1:15 p.m....(on a schedule) I hope you all know by now that people get jobs at least 85% of the time because of relationships...pretty easy to make 30 minute appointments with people all over the world. Think about contacting alumni... -
Summer SciComm Series: Master of SciComm
TypeBlog PostPublishedJuly 21, 2022, 1:15 p.m....the public’s perception of head injury risk. In completing a master’s thesis in science communication, I... Department of Science Communication where he completed a master's degree in 2020, his research explores... -
Using Addgene's New Viral Service for Your Research
TypeBlog PostPublishedNov. 17, 2016, 3:30 p.m....expertise you’d like to contribute to the blog, please email us and we’ll get you writing! www.addgene.org... Service or any of the materials we distribute, please send an email to [email protected]. Your questions...