We narrowed to 861 results for: REN;
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TypeBlog PostPublishedJuly 17, 2018, 12:23 p.m....the game to each a variety of subjects at many different education levels. If you modify BUILD YOUR PLASMID...us at: [email protected] Marco Straccia is currently an Associate Professor at University of Barcelona...
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FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog PostPublishedMay 21, 2019, 12:10 p.m....in living animals. To do this they modified a different self-assembling split GFP (GFP1-9 and GFP10-11...plasmids for FlipGFP and FlipCherry at Addgene. References Cabantous, Stéphanie, et al. "A new protein-protein... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog PostPublishedApril 28, 2021, 1:15 p.m....same features, but this chart highlights the differences between these two citation managers. If you want...a favorite writing tool, let us know below! References and Resources You Can Grad School podcast episode... -
25 Work From Home Tips for Scientists
TypeBlog PostPublishedMarch 19, 2020, 1:15 p.m....things you can do to prepare. Adapt to WFH while parenting Keep your kids on a schedule so you can rely ...Most Micropublications contain one figure. If you aren’t there yet, write introductions to potential publications... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog PostPublishedFeb. 20, 2018, 2:30 p.m....multiple samples without cross contamination. We currently use the CosMCPrep system for this, but thought... because it forced workshop participants from different biotech companies to interact and learn more about... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog PostPublishedAug. 13, 2015, 2:30 p.m....the interview process was several short talks by current faculty members and it just blew me away…. I have...overall mission of making science easier and more transparent. That’s largely my job [at the Broad] too… to... -
Finding and Joining Your Dream Lab
TypeBlog PostPublishedApril 8, 2014, 2:50 p.m....interests, and spends one-on-one time with the current members to share short-term goals and chat about..., but even brief stays there can make all the difference. Choose a lab that brings on the happy dance ... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog PostPublishedApril 27, 2023, 1:15 p.m....projecting to the inferior colliculus (IC) are differentially transduced when using the retrograde serotype...significantly updated in 2023 by Jason Nasse. References 1. Tervo, D. Gowanlock R., et al. "A designer... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog PostPublishedMarch 21, 2023, 1:15 p.m....deliver cargo, it’s important to understand their differences to choose the best one for your experiment. ...Here to Find Viral Vector Resources at Addgene! References and Resources 1. Roe T, et al. “Integration of... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog PostPublishedJune 29, 2017, 1:06 p.m....and mEGFP. Many of these features, such as Cas9, weren’t detected by our in-house software. In fact, SnapGene...static image file (PNG, see above) for your own reference or to paste directly into your notebook. This ... -
Hot Plasmids - May 2022
TypeBlog PostPublishedMay 26, 2022, 1:15 p.m....peptide. By combining three different localization peptides with four different fluorescent proteins, and... -
Antibodies 101: ChIP
TypeBlog PostPublishedMarch 10, 2022, 3:25 p.m....optimized for each specific sample type. And, if that weren’t bad enough, optimization of one step can affect...protocols when embarking on your ChIP journey! References and Resources More resources on Addgene’s blog... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog PostPublishedJan. 8, 2019, 1:54 p.m....illustrates the utility these tools have in many different areas of biology as they grow. The top 15 plasmids...envelope protein enables this virus to infect many different cell types so it is useful in a wide range of ... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog PostPublishedJune 30, 2016, 2:30 p.m....contain 3 methylases that recognize and methylate different stretches of DNA: Dam methylase adds a methyl...could overlap with a methylation site. The quick-reference table below lists 10 common enzymes that may be... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog PostPublishedJune 2, 2016, 2:30 p.m....your working directory (the folder that you are currently working in) every time you use them, leave them...guest blogger Kate Palozola! Kate Palozola is currently a graduate student at University of Pennsylvania... -
Addgene: The First Twenty Years
TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....organizations, knocking on doors, and attending conferences. And when CRISPR arrived in 2012, our repository...’s booth at the Society for Neuroscience 2023 conference. In 2020, the world changed in response to the... -
Plasmid Tools for Microbiome Studies
TypeBlog PostPublishedJune 27, 2019, 12:25 p.m....and Gammaproteobacteria species isolated from different bee species. BTK can be used to introduce fluorescent...distributed cell division counting plasmids here References Leonard, Sean P., et al. "Genetic engineering... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....transfer, each GOI is usually expressed from a different plasmid, which may lead to unequal gene dosage...consists of 4 plasmid toolkits generated with 4 different, binary 2in1 vectors with all possible combinations... -
Visualizing Protein Turnover In Situ
TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m....whether or not the tags influence behavior. A conference and a breakthrough! During my graduate studies...you can contact her at [email protected]. References 1. Dieterich, Daniela C., et al. "In situ visualization... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog PostPublishedDec. 8, 2016, 3:30 p.m....probably aware that there are several methodologies currently available for approaching it. These include the...even to submit a guest blog entry of your own! References 1. Bernard, P. “Positive Selection of Recombinant...