We narrowed to 813 results for: Ran
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TypeBlog PostPublishedOct. 20, 2016, 2:30 p.m.... general advice on how to meet people, how to transition out of academia, and other science career topics.... Although I didn’t start answering until 5pm, frankly, I was typing far before five. Fortunately I have...
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The Challenges of Cell Culture
TypeBlog PostPublishedMay 5, 2016, 2:30 p.m....cell line contamination: Epithelial Mesenchymal Transition Is Required for Acquisition of Anoikis Resistance... to carry our independent quality control for a range of testing, from animal doping to forensics, DNA... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog PostPublishedSept. 5, 2017, 1:58 p.m....complex is very efficient at introducing double stranded breaks (DSBs) into genomic DNA in many cell types...homologous DNA repair template, such as a single-stranded oligodeoxynucleotide (ssODN), which contains the... -
An Integrin Antibody Toolkit from IPI
TypeBlog PostPublishedMarch 28, 2024, 1:15 p.m....heads and long legs that protrude from the cell membrane, physically connecting the extracellular environment...The actin cytoskeleton exerts tensile force, transmitted through the integrin, where it is resisted by... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog PostPublishedSept. 1, 2015, 2:30 p.m....will take to secure your first (R-level) research grant. Set your timeline from there, then start identifying...techs’ financial books. To find personnel salary ranges, go to online job information sites (like, indeed.com... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog PostPublishedNov. 7, 2014, 2:56 p.m....Propagation of ccdB-containing plasmids Hte High transformation efficiency deoR constitutive expression of...+), λ– EPI300 Cloning large inserts and transformation of large plasmids. F- mcrA Δ(mrr-hsdRMS-mcrBC... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog PostPublishedMay 23, 2023, 1:15 p.m....palette for display (like green-magenta-blue or orange-blue rather than red-green). Even better, show ....2846 Radtke, A. J., Kandov, E., Lowekamp, B., Speranza, E., Chu, C. J., Gola, A., Thakur, N., Shih, R... -
Finding Your Perfect Job After University
TypeBlog PostPublishedJan. 12, 2016, 3:30 p.m....project to validate some genomic changes, so this arrangement was ideal for everyone. The cutting edge science...young scientists and PhD students to also explore a range of alternative careers outside of acidemia to have... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog PostPublishedNov. 3, 2015, 3:30 p.m....should we use all tools as some kind of a meta/Frankenstein app? Or perhaps there is a clear winner, is ...enthusiasts, take a look at sgRNAcas9. Top tools for transferring CRISPR technology to a new organism: For obvious... -
#DeckTheLab to Win a Tasty Winter Treat This Holiday Season
TypeBlog PostPublishedNov. 30, 2015, 3:30 p.m....mini marshmallows…). We’ll choose 4 submissions at random and deliver the prizes at the start of the New ... -
Hot Plasmids: Bacillus subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!
TypeBlog PostPublishedDec. 11, 2017, 2:02 p.m....on the B. subtilis libraries. Hot Plasmid 2: Transcription factor fusions modulate iPSC reprogramming efficiency... -
Hot Plasmids Podcast Episode 3: Optogenetics Tools, Improved CRISPR Base Editors, & More!
TypeBlog PostPublishedOct. 25, 2017, 1 p.m....Hot Plasmid 3: CRISPR base editors with enhanced range and specificity. Listen to to the Segmennt of Improved... -
Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More
TypeBlog PostPublishedSept. 8, 2017, 7:52 p.m....Gateway-Compatible Toolbox for quickly generating zebrafish transgenic lines to study neurodegenerative diseases. Listen... -
Suggest A Plasmid You'd Like to Find at Addgene
TypeBlog PostPublishedMarch 16, 2016, 5:30 p.m....where we can find the plasmid. While we cannot guarantee that everyone we reach out to will end up depositing... -
The 12 Days of CRISPR
TypeBlog PostPublishedJan. 3, 2023, 2:15 p.m....Noninvasive assessment of gut function using transcriptional recording sentinel cells! 🎵 On the 11th day... -
New Videos: Addgene Lab Tips
TypeBlog PostPublishedFeb. 15, 2018, 2:41 p.m....hopefully make your life a little easier. These can range from technical tips as in our episode on Barcoding... -
Twenty Years of Sharing Science!
TypeBlog PostPublishedJan. 8, 2024, 3:25 p.m....science support to our efficient and easy tech transfer process to our website and educational resources... -
Expanded Access to Rabies Plasmids
TypeBlog PostPublishedFeb. 1, 2024, 2:15 p.m....where Addgene is located. The export license, if granted, allows us to ship rabies plasmids and viral vectors... -
Celebrating the 2024 Nobel Prize in Chemistry
TypeBlog PostPublishedOct. 17, 2024, 1:15 p.m....References Kuhlman, B., Dantas, G., Ireton, G. C., Varani, G., Stoddard, B. L., & Baker, D. (2003). Design... -
New Educational Resource: The Addgene Videos Page
TypeBlog PostPublishedJune 16, 2017, 2 p.m.... that in mind, we are very excited to debut our brand new Addgene Videos page! Videos are a powerful ...