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  1. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...easier because individual labs will not need to ‘domesticate’ all the basic and necessary elements in order...purification; the whole cloning process is so simplistic once you have your parts. That was the motivation...

  2. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...Therefore, FPs are used to study many biological questions. The two most common uses are: 1) to test the ...microscopes the filters are not narrow enough to distinguish between closely related colors. Furthermore, ...

  3. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...viral vector production facility, food allergy testing lab, and the grad student down the hall from you...curve? A poor standard curve leads to over or underestimation of an unknown sample’s concentration. To determine...

  4. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...VSV-G due to wide infectivity, is often used as a substitute for HIV-1 Env, which can only infect CD4+ cells... for cancer immunotherapy, but this research is still in the pre-clinical/early clinical stages. Researchers...

  6. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ... one at a time by standard restriction enzyme digestion followed by ligation. Upon ligation, the original...and XhoI MluI BioBricks SpeI and XbaI EcoRI and PstI Bglbricks BglII and BamHI EcoRI Additional...

  7. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...genetic disease more prevalent or life-threatening (cystic fibrosis, hemophilia, muscular dystrophy)? AAV...Additionally, AAVs do not integrate in the host genome but still lead to long-term (>10 years) transgene expression...

  8. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...secondary nanobody, it’s important to review a few characteristics of your primary antibody: species it was raised...subclass. Once you know the answer to these two questions, refer to this table to find the right nanobody...

  9. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...PMCID: PMC5915492. Witte, Anna Kristina, et al. "A systematic investigation of parameters influencing droplet...

  10. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...labeling is to inject a mix of AAV vectors that constitutively express a red, green, or blue (RGB) fluorescent...studies), as well as translational applications (mechanistic studies and gene therapy treatments for neurological...

  11. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ... tag antibody produced by our partners at the Institute for Protein Innovation (IPI), part of a new collection... more highly autofluorescent tissues like the intestine. Figure 3: Expression of fluorophore-tagged...

  12. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...far messier than biorender plots, quadrants can still help me as a reader if I'm struggling to see the...correlates with the occurrence of post-transplant opportunistic infection in liver and kidney recipients. World...

  13. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...expressing it from a CAGGs promoter. However FLPe is still less efficient than Cre recombinase. FLPe codon ...Postdoctoral Researcher at MIT and is fascinated by the question of how genes and proteins coordinate billions ...

  14. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ... and Mary Gearing on Mar 12, 2015; updated by Christina Mork, Jul 27, 2020; updated by Susanna Stroik ...homology-independent pathway as it involves the direct ligation of sticky or blunt ends and often introduces small insertions...

  16. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...biomolecules of interest e.g. DNA, RNA, and lipids. Still, these molecules can be indirectly visualized by...facilitates the choice of the proper site, although it still is worthwhile to generate several variants and check...

  17. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...Lambda Red recombineering, which uses enzymes domesticated from the Lambda bacteriophage, is arguably the...that have only gone through the single cross over still contain sacB and will not grow on sucrose. One ...

  18. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ... expression is dependent upon the cell type in question. In plant cells, gRNA expression is achieved by...depending on the delivery method and cell type in question. CRISPR components can be delivered and expressed...

  19. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...SV40, are always active and thus referred to as constitutive promoters. Others are only active under specific... GAL80 under different promoters and create sophisticated patterns of UAS-driven gene expression. To further...

  20. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...1896) where he explores the concepts of animal testing and modifications. The premise of the novel involves...mutations. In 2016, the researchers at the Broad Institute envisioned a new strategy to introduce point mutations...
Showing: 701 - 720 of 835 results