We narrowed to 852 results for: STI

... clade of huge phages. In this article, they investigated three Casɸ orthologs, Casɸ-1, Casɸ-2, and Casɸ...photostable yellow fluorescent protein to date By Justin Ng High-throughput screening methods, such as fluorescence-activated...

...six different genes whose protein products have distinct localizations. Figure 2: Split-wrmScarlet...the protein to fluoresce red. H2B-FT-red is long lasting and was shown to have a half life of 84 hours in...

...sequences with G/C content outside this range can still be optimized (Anzalone et al., 2019). Test different... edit. If possible, consider following these suggestions: Edit the PAM along with your intended edit....

...modifying enzyme) to pair with it can be queried by testing any number in the Level 2 assembly. These pathways...scratch! As you can imagine, there is an array of distinct goals within metabolic engineering, synthetic ...

...This new capsid will be a valuable tool for investigating NHP brains. Figure 3: Overview of library...Importantly, AAV1-X1 is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice...

...mammalian cell expression, and compatible with constitutive or Cre-dependent expression. These sensors have...the calcium sensor to specific tissues or even distinct cell subtypes for efficient expression and downstream...

...used to expand already-existing sets. Note that this tool relies on a stochastic search algorithm rather...

...sample protein is retained better, antibodies will stick permanently to a membrane that has been dried, no...harsher stripping means more sample loss. If you are still optimizing your system, start with a mild stripping...

...limited to restriction enzymes - although many labs still love using them! With so many technologies currently...an ordered set of scarless ligations following digestion with Type II restriction enzymes. All of these...

...cross-linked, DNA is digested into smaller pieces. Sticky ends created are then ligated together to create...interactions opens the door for answering more questions about chromatin conformations. The Xu lab presented...

...prime editing in both tomatoes and Arabidopsis, suggesting the improvements may be broadly applicable to...activation kinetics and high responsivity near the resting membrane potential, ASAP5 is a great new option...

...transfection reagents and keep the cells happy. Harvesting virus Once you have transfected your cells, wait...non-genome-containing (empty) capsids, thus leading to an overestimate of transducible units in the virus preparation...

...proteins, it’s important to note that despite their distinction by name, chromoproteins are actually a subset...wavelengths of low photosynthetic activity. This still allows photosynthetically active wavelengths to ...

... lead to a wealth of additional experiments investigating the role of individual genes or entire pathways... you use them for! A well-developed biological question and experimental system are absolutely necessary...

...easier because individual labs will not need to ‘domesticate’ all the basic and necessary elements in order...purification; the whole cloning process is so simplistic once you have your parts. That was the motivation...

...Therefore, FPs are used to study many biological questions. The two most common uses are: 1) to test the ...microscopes the filters are not narrow enough to distinguish between closely related colors. Furthermore, ...

...genetic disease more prevalent or life-threatening (cystic fibrosis, hemophilia, muscular dystrophy)? AAV...Additionally, AAVs do not integrate in the host genome but still lead to long-term (>10 years) transgene expression...

...secondary nanobody, it’s important to review a few characteristics of your primary antibody: species it was raised...subclass. Once you know the answer to these two questions, refer to this table to find the right nanobody...

...labeling is to inject a mix of AAV vectors that constitutively express a red, green, or blue (RGB) fluorescent...studies), as well as translational applications (mechanistic studies and gene therapy treatments for neurological...

... one at a time by standard restriction enzyme digestion followed by ligation. Upon ligation, the original...and XhoI MluI BioBricks SpeI and XbaI EcoRI and PstI Bglbricks BglII and BamHI EcoRI Additional...