We narrowed to 750 results for: Tro;
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TypeBlog PostPublishedFeb. 28, 2019, 1:34 p.m....practices for reproducibility: data management plans, electronic lab notebooks, wet lab protocols, reagent sharing...
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Summer SciComm Series: Cognitive Load
TypeBlog PostPublishedJune 16, 2022, 1:15 p.m.... audience. More resources on the Addgene blog Intro to our Summer SciComm Series... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog PostPublishedAug. 5, 2021, 12:15 p.m....in pMinDisplay (PDGFR anchoring domain), in electroporated in rat hippocampal cultured neurons, imaged... -
Visualizing Protein Turnover In Situ
TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m....protein synthesis and degradation. These processes control the abundance of proteins in cells. Homeostatic... -
Developing Lab Management Software for Biology
TypeBlog PostPublishedAug. 25, 2016, 2:30 p.m....individually keep track of any failures and do troubleshooting on their own. For a few samples this isn’t ... -
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog PostPublishedDec. 16, 2015, 3:30 p.m....Created with BioRender.com. To decrease the electropositivity of the HNH/RuvC groove, Slaymaker et al. made... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog PostPublishedSept. 22, 2015, 2:30 p.m....similar to an academic research environment” with a strong focus on publishing, but still made it so she could... -
Engaging with science and society at pgEd
TypeBlog PostPublishedSept. 20, 2018, 12:17 p.m....communication posts Extracurricular activities for a strong science career path Resources on Addgene.org ... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog PostPublishedJune 26, 2018, 1:33 p.m.... Decades ago, other labs discovered that the in vitro functions of 11 different Arg-mutant enzymes could... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....plasmids — more on that in a minute! Fun fact! Electroporation is one popular method scientists use to promote... -
Making CRISPR Plasmids Using Fragmid
TypeBlog PostPublishedAug. 29, 2024, 1:15 p.m.... in the hands of the Doench lab. Additionally, controls available in the system are validated and were... -
How to Prepare for an Industry Interview
TypeBlog PostPublishedJuly 9, 2020, 1:15 p.m....allow it. Before you start your presentation, introduce yourself to your audience. Pay attention to... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog PostPublishedAug. 24, 2021, 1:15 p.m....techniques that you can use antibodies for in our Intro to Antibodies post Resources on Addgene.org Browse... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog PostPublishedDec. 1, 2015, 3:30 p.m....boiling point of water. Dr. Terns gave a great introduction to the repeated and distinct aspects of the ... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog PostPublishedNov. 19, 2015, 3:30 p.m....living cells where methylation of these sites was controlled, the reporter consistently showed significantly... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog PostPublishedOct. 11, 2018, 12:30 p.m....using similarly sized Lambda amplicon inserts to control for any suppression of background by antibiotic... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog PostPublishedAug. 21, 2018, 12:31 p.m....give Gateway a try. This method, developed by Invitrogen and based on lambda phage integration sequences... -
10 Basic tips for mammalian cell culture
TypeBlog PostPublishedJuly 12, 2018, 1:09 p.m...., freeze them, and store all but one in liquid nitrogen. The frozen vials make up your master stock, while... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog PostPublishedSept. 7, 2017, 12:17 p.m....expression. We highly recommend the use of an electronic pipettor with variable spacing (such as the Viaflo... -
Viral Vectors 101: Producing Your rAAV
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m....cell-associated (Vandenberghe et al., 2010). For certain in vitro and in vivo applications, you can consider using...