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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 845 results for: ins

Showing: 701 - 720 of 845 results

  1. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ... the DNA prep is larger. And since the library contains many plasmids, care must be taken to ensure that...plasmids in the library are identical except for the insert; there’s no need to sequence other parts of the...

  2. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...help unlock the potential of modern sequencing instruments by enhancing the efficiency and simplicity of...completely unnecessary for sequencing multi-kb DNA inserts and larger targets. The NGS readout also reveals...

  3. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...never finish a course of antibiotics, choosing instead to save a few days’ worth of capsules to self-medicate...much of the information gained from that research remains inaccessible, behind paywalls and in technical ...

  4. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...models for immunogenicity prediction of therapeutic proteins. European journal of pharmaceutics and biopharmaceutics...biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 130, ...

  6. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...need to present yourself using a resume. Indeed, instead of having all the pages in a Curricula Vitae to...career, she transitioned into a communications role instead after she completed her MSc in Experimental Medicine...

  7. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...communicates your interest in a specific program, and institution. Consequently it’s best to draft a cover letter...or mentor. Several days prior to the interview, inspect your technology, background, and lighting for a...

  8. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...months: Lentiviral preps for expressing SARS-CoV-2 proteins ChR2 for targeted expression DREADDs GFP dependent... quickly shared plasmids expressing SARS-CoV-2 proteins at the beginning of April. Now, we've turned some...

  10. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ... coexpression of light (green) and heavy (blue) chains as driven by two CMV promoters (orange). Figure... to WGS the lab’s entire hybridoma collection. Instead, the Trimmer Lab moved to direct functional validation...

  11. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...for your workflow. Next time you make a mistake, instead of silently vowing never to do that again (I’ve...incubation periods to have another short "quiet period" instead of immediately running to the next thing.  It may...

  12. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...buffer components could affect the reaction. For instance, sodium azide, a common antimicrobial agent added...relatively rare protein in an experiment with many proteins of interest! Research the brightness of your fluorophores...

  13. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    .... Projects such as the OpenFlexure Microscope (Collins, et. al., 2020), the miCube, SQUID (Hongquan, et...and low-cost optical setups. References Joel T. Collins, Joe Knapper, Julian Stirling, et. al, "Robotic...

  14. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al., 2014). It is a dual-vector system...viruses are popular, pAAV-hSyn-DIO-hM4D(Gi)-mCherry contains a double floxed human muscarinic receptor 4 (Gαi-coupled...

  15. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...gene knockout in vivo and in vitro, as well as to insert targeted mutations through homologous recombination...effects and/or toxicity, it would be advantageous to instead use a preexisting, conserved cellular machinery...

  16. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...journal. An increasing number of funding bodies insist that the research data resulting from their funding...many journals use to verify whether a manuscript contains all the necessary information to be of maximal...

  17. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...interested in following? Someone you admire for their insight and way of expressing it in meetings? The more ...help you learn the local “ropes” and become an insider faster A mentor that works elsewhere is able to...

  18. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...biomedical researcher at Lunenfeld-Tanenbaum Research Institute, posting about Addgene’s 20th anniversary on Twitter...alike. We transformed this process by aligning institutions with standardized agreements and electronic ...

  19. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...in a variety of outcomes including excision or insertion, inversion, translocation or cassette exchange...addition, Casɸ has a minimal PAM requirement. For instance, for Casɸ-2 the PAM sequence is 5’-TBN-3’ (where...

  20. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ... a STAR answer once the interview has started. Instead, I preferred to prepare stories to answer interview...possible. Otherwise, read the video conferencing instructions you received from HR carefully. And even if ...
Showing: 701 - 720 of 845 results