We narrowed to 711 results for: tat
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TypeBlog PostPublishedJan. 24, 2023, 2:15 p.m.... acoustic contrast in E. coli. We deleted an unannotated hypothetical protein, improving expression, and...
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Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog PostPublishedOct. 19, 2017, 1:54 p.m....one of the FLP-FRT systems in your experiments. Mutated FRT and loxP sites in Flex Vectors – inversion ... -
Viral Vectors 101: Pseudotyping
TypeBlog PostPublishedApril 20, 2021, 2:30 p.m.... Q, Liu Q, Huang W, Li X, Wang Y (2017) Current status on the development of pseudoviruses for enveloped... -
An Inside Look into Addgene's Dev Team
TypeBlog PostPublishedJan. 23, 2020, 2:45 p.m....provides transparency to everyone involved about the status of each project. This creates accountability on... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog PostPublishedNov. 1, 2018, 12:35 p.m.... a GFP cassette, and introduced site-directed mutations, all without introducing unwanted selection markers... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog PostPublishedOct. 13, 2016, 2:30 p.m....pointed feedback or abruptly change needs and expectations. Such situations can get stressful and to help... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog PostPublishedMarch 5, 2019, 1:08 p.m....truncated, non-fluorescent form of YFP as the default state of the transgene such that recombination is necessary... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog PostPublishedJune 3, 2021, 1:15 p.m....intracellular T cell receptor portion of the CAR facilitates signal transduction and release of cytotoxic ... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog PostPublishedJune 17, 2025, 1:15 p.m.... to knock down genes with potentially harmful mutations in therapeutic settings. Silencing of a target... -
Antibodies 101: Beyond Surface Labeling
TypeBlog PostPublishedJune 27, 2024, 1:15 p.m....cross-linking, killing the cell but preserving the cellular state (think of mummification). Permeabilization, as the... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog PostPublishedJuly 19, 2016, 2:30 p.m....PMC2735349. 8. Miyawaki, Atsushi, et al. "Dynamic and quantitative Ca2+ measurements using improved cameleons."...