...once got a box of 6 perfect, chocolate-covered strawberries for helping with an introduction that helped... have been enough, but I’ll never forget the strawberries. Grand gestures are memorable! Attend events... want to do: Don’t connect via LinkedIn with strangers. It’s a waste of time. People that don’t know ...

...IF-ICC.) This method was chosen because cells transfected with a plasmid to express the target antigen ...was recognized that an aberrant IgG light chain transcript was expressed by some of the hybridomas and, ...Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD...

...via globally established makerspace networks and training users via workshops, seminars, or hackathons greatly...centers provides a potential conflict between traditional IP-based models and the use of taxpayer money...next few years, with the goal of creating more transparent and reproducible science in the long run (Hohlbein...

...fluorescent reporter for Erk kinase translocation. These results demonstrate that OptoNBs can be harnessed ...With darkness, SOS binds Ras and initiates the extracellular signaling-regulated kinase (ERK) signaling cascade...

...than you – meeting scientists at your level of training is always a good idea. Make contact with other... may start in the airport. If you see someone traveling with a poster roll, say hello. Keep your cards...just a meeting, but having lunch twice turns a stranger into a friendly colleague. Recommended Reading...

...lauded by another set of stakeholders - the tech transfer community. In a recent law review article we received...Work with a positive, supportive culture, we are attractive to the local employee pool and we get a lot of...inclusive workplace for everyone. We had Unbiasing Training in place before it was cool or a necessary result...

...the embryos underwent repair not from the single-stranded oligonucleotide (ssODN) template supplied by Ma...maternal allele rather than the supplied single-stranded oligonucleotide (ssODN) template. Embryos also...off-target effects, but this advantage likely won’t transfer to all genes we’d like to target. In addition,...

.... “I was lucky to have been able to make that transition really smoothly,” says Susan. After being in ...sucked to work remotely. I was also tired of the contracting life where you do a little bit of work for one...resume, she decided to complete a certificate in Strategic Communications and Public Relations at the University...

...expressed in transfected eukaryotic cells, allowing for them to be used as intracellular inhibitors.  ...Smaller, slimmer affinity reagents can have deeper penetration into a tissue, useful for applications like IHC...

...Melina Fan, a cell biology graduate student, was frustrated by the difficulty of accessing published plasmids...Part of that work meant facilitating the Material Transfer Agreement process, which was a major barrier for... for depositors and requestors alike. We transformed this process by aligning institutions with standardized...

...make your own antibody fragments as needed.  In contrast, anti-IgG antibodies, commonly used as secondary...’ fragments are almost interchangeable, but the extra bit of heavy chain on a Fab’ can act as a convenient... expression and will have the highest tissue penetration. However, they tend to be less stable and have...

...institutional subscription), archived on PubMed Central, or in preprint onBioRXIV. One of the biggest issues... the action. If the paper is well-written, the abstract should give you a sense of the importance of the... the dark ages with respect to my organization strategy. There are a number of software options for helping...

...myasthenic syndrome (LEMS) because, at high concentrations (25 mM), Gdm+ binds to and inhibits a potassium...living cells. The McMurray lab found that low concentrations (≤3 mM) of GdnHCl can functionally rescue multiple...available from Addgene. Dr. McMurray hopes that by demonstrating GdnHCl chemical rescue of a variety of disease-linked...

...sequences that, when bound by transcription factors, can increase a gene’s transcription. Sometimes enhancers ...differentiation. Using a cell line model, the Xu Lab tracked enhancer-promoter interactions during erythroid...

...schedule. If there is a list of poster titles or abstracts available before the conference, comb through ...notes or questions from your poster viewers and trade contact information if your conversation is particularly...overwhelming when you are presented with a sea of strangers. Try things like sitting at a table for lunch ...

...other words, newborn poop) visible through their translucent bodies. This is the ideal stage to select for...recombination can potentially cause you to lose track of your mutation. To maintain stable lines of flies... transgenic flies...

...fluorescent dye that works by binding to all double-stranded DNA, while Taqman uses an intercalating dye, with... the readout gives an absolute value of DNA concentration. By comparing the number of positive droplets...gene, copy number can be determined through concentration values, rather than by comparison to a standard...

...of current new and exciting literature. As I transitioned out of academia and away from the bench I certainly... decide on the topic, there are a number of administrative and practical decisions to make. How often ...presenter, figure by figure) if the primary purpose is training graduate students in critical reading. If the ...

...has grown, it has taken effort to continue the tradition and plan enjoyable events for employees. We now..., evite, posters, megaphone, or your company’s intranet. Don’t forget about the power of simple word of...people excited!    Laura Veckerelli is the Administrative and Operations Coordinator at Addgene. In addition...

...usually smallish peptides incorporated into a translated protein. As depicted in the accompanying cartoon...peptide-based proteosome inhibitors, glutathione S-transferase (GST), which can be fused to recombinant proteins... quickly – modifying the length a polyhistidine tract does not greatly alter the size of the tag.   Table...