...rules are: (1) don’t use the same overhang twice; (2) avoid palindromes; (3) no overhangs with the same...highest-fidelity overhang set it can find for those parameters. For even more specificity, you can exclude specific...

...vectors with EYFP split at 175 Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components...

...systems utilize with type I-F Cascades. Figure 2: Cascade complex from CRISPR type I-E. Created with...been challenging. It can be difficult to ensure comparable expression of all components of Cascade for maximum...

...that view (Callahan et al., 2022).     Fig. 2: Students in an MDH CURE replaced select residues ...Part I – guided inquiry – purification and characterization of a fusion protein: Histidine tag, malate...

...interesting things to do, feel free to crowdsource! 2. Free food generally gets people to show up If you... company culture here at Addgene has remained paramount. Candidates who interview at Addgene frequently...

...experience and to build upon your existing skills.  2. Find your niche Identify and develop an area of expertise...seekers to use online applications with significant character limits for the written portion of the grant application...

...based antiviral method to degrade RNA from SARS-CoV-2 sequences and live influenza A virus. It was demonstrated...stuck in inclusion bodies. Takayuki Iwaki, Kimiko Hara, and Kazuo Umemura have described a simple method...

...rate of 10-55% - the PASTE system.     Fig. 2: Schematic of programmable gene insertion with PASTE...and Discovery As part of their quest to find, characterize, and develop new CRISPR systems and technologies...

...externally fertilized, transparent progeny (Figure 2). These features give it a leg up (or a fin up?) over...time and space, and was also used to precisely characterize developmental defects in the one-eyed pinhead...

...two visible blue or magenta chromoproteins (Figure 2) (Liljeruhm et al., 2018). This system is a nice confirmation...tested their expression under both rhamnose- and arabinose-inducible promoters. Tight control of toxin expression...escape). The rhamnose induction system is well-characterized and quite tight (Giacalone et al., 2006). The...though Khetrapal et al. also demonstrated that the arabinose promoter worked too. Rhamnose induction relies...References Espah Borujeni, Amin, Anirudh S. Channarasappa, and Howard M. Salis. "Translation rate is controlled...