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We narrowed to 810 results for: ARC;

Showing: 361 - 370 of 810 results
  1. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...products that resulted from their research, “aha” moments, and future research directions. Don’t worry if your...this file over the years and a talk about your research or work will write itself when the time comes....ask about how many people use plasmids in their research and then see how many of them have requested from...
  2. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...Originally written by Marcy Patrick and Mary Gearing on Mar 12, 2015; updated by Christina Mork, Jul ...site-specific knock-in tool that can be harnessed by researchers for many applications. Your favorite Cas enzyme...generally low; to increase the frequency of edits, researchers have designed self-cleaving plasmids that liberate...repair pathway choice. Shrivastav M, et al Cell Research (2008). PubMed. Mechanisms of DNA double-strand...
  3. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...blue light-activated cation channel, from the archaebacteria Chlamydomonas reinhardtii. When exposed to ...combination with retinal) variations designed to give researchers flexibility in using optogenetic tools in their...concentration gradient, and of activation inhibitory (ArCH) proton pump passing ions against the concentration...proteins activate their signaling pathways allowing researchers greater control over when and where to activate...
  4. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...increasing affordability and accessibility, more researchers have been utilizing full plasmid sequencing services...sequencing that Addgene uses.) However, when some researchers received the sequencing results for their plasmids...numbers (Williams, Carnes, and Hodgson, 2009). More research is ongoing to determine plasmid features that ...monomeric plasmid compared to DH5α. However, more research is needed to determine which recA- strains are...
  5. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...135. https://doi.org/10.12688/f1000research.21018.1 Munoz-Lopez M, Garcia-Perez J (2010) DNA Transposons...different transposon systems adapted for use as research tools: Sleeping beauty, PiggyBac, and Tol2.  Sleeping...encode reporter genes, mutagenic cassettes, or barcodes. When transposons are delivered to cells or a ...
  6. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...how and where you pursue it. It can manifest via research and data gathering, regulatory efforts, policy...fellowships have a strong focus on early career researchers who have decided to transition out of academia... host “Hill Days” where they train and bring researchers to Congress to advocate for increased science...incentivizing the real-world impacts of health services research. She is particularly interested in driving scientific...
  7. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ... design of these constructs, allowing novice researchers to quickly and easily start genome editing (Schwartz...donors in cultured Drosophila cells. Nucleic Acids Research 42:e89–e89 . https://doi.org/10.1093/nar/gku289...CRISPR-targeted homologous recombination. Nucleic Acids Research 41:e193–e193 . https://doi.org/10.1093/nar/gkt805...Tzur YB, Friedland AE, Nadarajan S, Church GM, Calarco JA, Colaiácovo MP (2013) Heritable Custom Genomic...
  8. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...effectiveness and interpretation of RNAi. Fortunately, researchers studying plants and other organisms lacking this...concatenated. hpRNA has been extensively used in plant research and many different constructs are available to...and is a popular RNAi trigger in the mammalian research community. Fortunately, many tools for shRNA in...fluorescence readout of RNAi activity. As a plant researcher, the traditional hpRNA and visual screening method...
  9. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...efficiency. Together, these factors prevent stem cell researchers from generating clonal knock-in lines, especially... allele. For N-terminal tagging, specifically searching for error-free knock-in at both alleles may be...for Regenerative Medicine (CRM), Edinburgh. His research interests include optimizing CRISPR/Cas9 genome-editing...Dewari PS, Kalantzaki M, Gangoso E, Matjusaitis M, Garcia-Diaz C, Blin C, Grant V, Bulstrode H, Gogolok S...
  10. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ... an important animal model for research, particularly genetics research, for decades. Its short life cycle...filmed at Addgene:     If you're working in fly research, I hope you find this new technology as helpful...
Showing: 361 - 370 of 810 results