We narrowed to 904 results for: CAN

...hybridoma cell lines can produce more than one mAb (Bradbury et al., 2018). Hybridomas can lose gene expression...into a fully functional antibody. These antibodies can be used just as you would use antibodies made from...purified antibodies from a patient’s blood), scientists can use mass spectrometry to determine the amino acid...from an established hybridoma line, the antibody can be converted into recombinant form through DNA sequencing...2010; Andrews et al., 2019). Finally, scientists can perform the entire process of antibody selection ...been cloned into an expression plasmid, the plasmid can be introduced into host cells, such as bacterial,...engineer them. Given the ease with which scientists can now improve genetically-encoded research tools, it...

...what facet of academia you are in, grant writing can be the lynch pin that results in your success or ..., and perhaps the most important bit of advice I can suggest, is to give yourself plenty of time when ...and why these findings will be worth knowing? This can sometimes be tricky, as most of our research builds...collaborate with specialists in different fields that can provide different insight into an area - not just...Furthermore, using new technologies to solve a problem can allow you to approach that problem in a novel way...results published in a paper, but positive outcomes can include poster presentations, talks about your work...grant application to your peers and collaborators can also help you get some critical insight into what...

... tools can distinguish between global strains of the Zika virus (Asian-, African- and American-lineages...monitor and slow this spread until vaccine programs can be put in place to provide community protection. ...allow for the rational design of RNA sensors that can detect virtually any RNA sequence (Green et al, 2014... later. The Zika publication timeline I think we can speak for all the authors on the paper when we say... of a rapid design and prototyping pipeline that can take pathogen sequence information from public databases...technology on active virus, as well as shown that we can detect the virus in a clinically relevant setting...patient blood and urine samples; we showed that we can detect the virus directly from infected monkey blood...

...need. You can find all the hot plasmids from 2016 below. With over 45,000 plasmids, we can't write posts...biotinylate proximate proteins in the cell. These candidate proteins can be enriched via biotin pull-down methods...transcription from the minimal promoter and its strength can be determined from its enrichment over input levels... (the identity of the genes and target sequences can be found here). To use the library, the authors packaged... CRISPR libraries from Addgene’s repository that can be used to perform screens in human cells. General... well as criteria of a good screening experiment can be found on our blog post entitled “Genome-wide Screening...Detailed information regarding the Wu Lab library can be found in the associated publication. Ma, et al...

...Experiments using enzymatic labeling can be difficult to quantify accurately, but can be especially helpful to generate...imaging, repeating for each target. Alternatively, you can perform simultaneous detection with a mixture of ...secondary has a different color fluorophore that you can image separately on your microscope. Abcam's sample...important consideration when deciding whether you can do simultaneous or sequential labeling is what conjugated... fluorophores, then labeling with the other, you can still record the signal separately for each target...specificity, and dye meets your needs and budget, or you can make your own. For example, to label the rat brain... automatically from the microscope software, you can always recolor them! In Fiji, for example, you’d ...

...include CRISPR machinery (which can be supplied separately). Libraries can target specific pathways or the...lineage tracing, among other applications. Cells can be identified using fluorescence, like in the pMuSIC...that prevent their expression. The knockout cells can be used to identify genes that underlie a particular...CRISPR isn’t the only game in town! Pooled libraries can be designed for all kinds of different high-throughput...with a pooled library. Surface display screening can also be conducted with yeast. Created with BioRender.com...

...replication can involve an RNA or dsDNA intermediary, depending on the class of virus. RNA genomes can be transcribed...removed. This means the vector can deliver a genetic payload to a cell but cannot create new particles. As ...have a wide variety of possible genome types that can be either double stranded (ds) or single stranded...Image credit: Louten, 2016 Viruses with DNA genomes can either follow central dogma (gDNA to RNA to proteins...were able to create far safer viral vectors that can be used in BSL-1 or BSL-2 labs. Viral vectors are...shell (Taylor, 2014). They’re so simple that we can’t even decide if they’re alive or not. Yet these simple...

...as yeast. HEKs can also grow in suspension, which allows for large scalability, and can also be adapted...cells can catch a cold—or worse! HEK-293T and its derivatives are immortalized, but cell health can degrade...reproducible results. It’s also widely available, which can make for a seamless transition of biological tools...degrade over time in culture. This can affect factors that could skew research results (gene expression... tumorigenic, which makes them a candidate cell line model for cancer-specific research. Membrane receptors...adapted for serum-free growth, which significantly reduces the cost of production. Research applications ...

...information so we can follow up with you if we have any questions and so that we can give you recognition...experimental details in research articles, scientists can use the AAV Data Hub to quickly find details they...next experiments using a specific AAV tool. They can search the database by certain features like serotype... the community. There are a few simple steps you can take to input your data for sharing on the site. ...injection and detection methods. For example, you can provide location, titer dose (Example: 1E13 GC/mL...

...semi-synthetic or fully synthetic compounds. Antibiotics can be categorized based on whether they directly kill...bit of a gray area as some bacteriostatic reagents can kill bacteria when used at high concentrations (and...antibiotic stocks, see Addgene's Reference Page. How else can antibiotics be used in the lab? Historically, antibiotics... for plasmid selection. Additionally, scientists can use the loss of resistance as a marker for successful...Carbenicillin is more stable than Ampicillin and can be used in place of Ampicillin in most applications... Simplify Your Cloning Experiments See Where You Can Find Antibiotic Resistance on Our Plasmid Pages ...