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  2. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...during in vivo research and the CARE guidelines for case reports are two sets of guidelines frequently used...manuscript is accepted for publication and is, in most cases, blind (meaning you don’t know who the reviewers...

  3. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...intensity remains. The first issue is that, in several cases, the fluorescence intensity decay of a fluorophore... the excitation power (Grünwald et al, 2008) occasionally and certainly after a set-up has been changed...

  4. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...primary-conjugated) or indirect (secondary) methods. In those cases, remember that a secondary should be used when signal...you still have a number of options. If this is the case, you can narrow them down in a variety of ways. ...

  6. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...given threshold within the cell, as is often the case upon viral infection. Endogenously produced dsRNA...the Addgene blog CRISPR 101: RNA Editing with Cas13  RNA Interference in Plant Biology  ...

  7. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...oligonucleotides specifying a gRNA target sequence into a Cas9-containing plasmid such as pX330. This cloning strategy...are using the Golden Gate method to create CRISPR/Cas9 constructs, assemble standard plasmids parts in ...

  9. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ... and serve as a type of positive control. In the case of an unfavorable donor-acceptor orientation limiting...molecules, detect cellular processes and signaling cascades, quantify mechanical tension (a molecular “spring...
Showing: 361 - 370 of 601 results