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Addgene

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We narrowed to 790 results for: Cre

Showing: 361 - 370 of 790 results

  1. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...suspected a data-driven approach might allow for an increase in the complexity. In 2018, they published a paper...conditions available in DAD tools While DAD made an incredibly complex reaction possible, NEB also optimized...Additionally, this assembly, which used only antibiotic screening for selection, failed under normal cycling protocols...Ultimately, data-driven Golden Gate Assembly greatly increases the potential complexity allowable by a favored...

  2. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...observe the effects. Luckily for us, CRISPR is an incredibly versatile system that can be tweaked to target...pairs long. Using a single guide RNA (sgRNA) to create a double-strand break works well because very small...ncRNA locus (Ho et al. 2012). For large-scale screening, sgRNA libraries are useful for targeting thousands...092902 Liu Y, Cao Z, Wang Y, et al. Genome-wide screening for functional long noncoding RNAs in human cells...

  3. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...OpenWorm OpenWorm is an open source project that is creating the “first virtual organism in a computer.” This...and split cGAL systems The Sternberg lab in 2016 created cGAL, a GAL-4 based bipartite expression system...reagent toolkit and plasmid assembly line for the creation of CRISPR targeting vectors that each contains...2016). Using SapTrap, this CRISPR plasmid can be created in a single tube. You can also find additional ...

  4. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...demonstrated to result in decreased mismatch tolerance and, consequently, increased specificity relative to...more stringent targeting rules that result in a decrease in available target sites. Regulating gene expression...focused advancing scientific discovery through the creation of innovative technologies. To date his group ...

  5. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ... you and your goals. Making a List of Schools Creating a list of schools is one of the first steps in...submitting your application. I found this step to be incredibly useful, as it gives a more comprehensive idea...advisor’s research interests which can be helpful in creating your application to that program. An email to ...simply not feasible. Through the virtual systems created and promoted during COVID-19, online meetings with...

  6. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...methods. While techniques such as  blue-white screening require X-gal, an expensive exogenously-added ...chromoproteins don’t require other substrates for screening. Thus chromoproteins can be used as markers in... used the amilCP and tsPurple chromoproteins to create negative selection markers for allelic exchange...transfer (FLIM-FRET) in living cells (Figure 4). They created ShadowR by modifying the surface of the chromoprotein...

  7. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...Hayward et al., 2024). The fluorescence intensity decreased whenever the magnet approached the sample (by ... and the team set up a directed evolution and screening experiment with three of the proteins that had...several rounds of semi-random mutagenesis and screening, the EGFP and mScarlet variants were showing no... suggest she deprioritize the project, Maria’s screens of AsLOV2 variants showed some significant gains...

  8. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ... we performed high-throughput ‘self-targeting’ screens with oligo libraries where pegRNAs are coupled ...cell contexts. Figure 1: High-throughput screening of pegRNA efficiency and building prediction tools...sequence-based prediction models from self-targeting screens such as PRIDICT/PRIDICT2.0 provide valuable insights...your target. Figure 3: High-throughput screening of the effect of chromatin on prime editing efficiency...

  9. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ... you typically would at a microbiology meeting. Create an outline: Write key bullet points for what you...while seated or with your poster on your computer screen than it is to pretend you’re standing at the session...off as a broken record, remember the outline you created for yourself. Hit the key bullet points and elaborate...will help you to provide an on-topic answer by creating time to organize your thoughts. It’s likely you...

  10. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...templates in somatic cells of adult animals in order to create animal models for particular cancer. That’s exactly...the Memorial Sloan Kettering Cancer Center did to create a model of Eml4–Alk-driven lung cancer (Maddalo... Addgene and could be useful to labs wishing to create this kind of animal models. Start using adenoviral...Circ Res 115:488–492 . https://doi.org/10.1161/circresaha.115.304351 Holkers M, Maggio I, Henriques SFD...
Showing: 361 - 370 of 790 results