We narrowed to 803 results for: Cre

...product and development teams have worked hard in increasing and improving accessibility on our website.  ... In fact, one of our team goals in 2023 was to increase accessibility to our website through the use of...describe images in online spaces. Alt text is used by screen readers to provide visually impaired visitors with...ultracentrifugation. The viral supernatant migrates through increasingly dense layers until the macromolecule density...

... preserving cell viability. Library delivery Screening libraries, large collections of biological independent...independent variables, are becoming increasingly popular tools with many applications. These libraries ...library cargo delivered are controllable. For many screening applications, it is essential to deliver a single...being explored as the delivery mechanism for increasingly popular nucleic acid-based vaccines. These vectors...

...sequencing by NGS. Addgene’s QC scientists immediately screen these plasmids to confirm they contain the expected...of plates will have select samples prepared and screened by NGS to spot check the samples, the rest will...are requested by scientists, will immediately be screened by our QC scientists to confirm the plasmids contain...By leveraging our core strengths of intensive screening and the use of high throughput platforms, Addgene...

...of Producing Monoclonal Antibodies, 1999). This creates an immortal cell population that will continue ...hybridomas for monoclonal antibody production. Image created with BioRender.com.   The antibody-producing... the genes from the hybridoma into a plasmid to create a recombinant antibody.     Figure 2: Changes...Generation of Hybridomas: Permanent Cell Lines Secreting Monoclonal Antibodies. https://www.ncbi.nlm.nih.gov...

...application of an electrical charge to cells, to increase cell membrane permeability and thus transformation...DNA can be accidentally packaged into the newly created phages. Transfer of this DNA to another cell is...Viruses (AAV). Lentiviral and AAV can be used to create both transient cell lines, where a gene of interest...Electroporation on the other hand uses electrical pulses to create transient pores in the cell membrane that genetic...

...Welcome to our deep dive series, which aims to increase your understanding and technical proficiency with...temperature drops, single intensity should smoothly increase as the DNA amplicon transitions from single-stranded...finally, drop once more during a final temperature increase. If there is a single amplicon, there should only...c) A double peak indicating 2 amplicons. Image credits (respectively): Zuzanna K. Filutowska; Selma, 2019...can fit in a quick game of Connect Four! Image created in BioRender.   Passive Reference Dyes Whatever...

...it, it just reflects how useful the reagents we created are for the community. More than 100 labs worldwide...need for high-quality service, open data, and increased reproducibility of materials. In March 2022, we... supported by an NIH BRAIN Initiative. We also created a growing library of antibody educational resources...York, Calico Life Sciences. Most of all, we’re incredibly thankful for all the scientists who have joined...

...ions flow into the neuron and causes it to fire. Created with BioRender.com. To make channelrhodopsin-based...information about parental channelrhodopsins to create recombinant channelrhodopsin sequences. The model...fiber-optic cable allows blue light to reach the brain. Created with BioRender.com. In the first experiment...neurons in the VTA, this should propel the mice to increase their nose-poking behavior. Strong nose-poking...

...Voigt: The big promise is orthogonality. You can create an almost limitless number of DNA binding proteins...fundamental. We built a NOR gate because they are used to create any program you can imagine. The Apollo 11 mission...so that genetic designs of that scale could be created. Someone could take on an engineering project including...enough hardware to control hundreds of genes. We’ve created enough regulatory elements, CRISPRs, repressors...

...observe the effects. Luckily for us, CRISPR is an incredibly versatile system that can be tweaked to target...pairs long. Using a single guide RNA (sgRNA) to create a double-strand break works well because very small...ncRNA locus (Ho et al. 2012). For large-scale screening, sgRNA libraries are useful for targeting thousands...092902 Liu Y, Cao Z, Wang Y, et al. Genome-wide screening for functional long noncoding RNAs in human cells...