...features of Addgene's website Resources on Addgene.org Try out the new search on Addgene's homepage ...Did you see Blugene’s make-over and our new homepage? If you've visited Addgene’s homepage recently, ...been working on behind the scenes here at Addgene.  Addgene's new homepage While users are increasingly...emphasize the values that make Addgene unique: community, sharing, and fun. The Addgene repository is built on....  Blugene gets a make-over You may also have noticed another big update on the homepage. Blugene, our... ways, we realized that Blugene needed the same thoughtful treatment. Blugene has been with us since 2011...might notice Blugene looking sad, especially if an experiment doesn’t go as planned. Blugene also has full...

...For example, this is an experiment using the chemogenetic tool hM4D(Gi) driven by a GFAP promoter in rat... rat. Contribute your AAV data Just like Addgene’s plasmid repository, the AAV Data Hub is a community-driven...only accepting data for materials distributed by Addgene.  Future directions As the AAV Data Hub grows, ...accelerate your research. Sincerely, Melina & The Addgene Team ...

...resources on the Addgene blog Learn more about about Addgene in our "Inside Addgene" blog series Subscribe...Subscribe to the Addgene blog Resources on Addgene.org Meet the other members of Addgene's Board of Directors... did you first hear about Addgene? How did you become involved with Addgene? Katz: Joanne and I first ...connection that got to know more about Addgene and heard that Addgene was looking for new board members. ... that Addgene provides the scientific community? Katz: The most important resource that Addgene provides...What do you hope Addgene will accomplish in the near future? Katz: I’m excited that Addgene is continuing... through Addgene’s sharing platform. Expanding further beyond the US and increasing Addgene’s international...

...Instrumental Access participants. Like Seeding Labs, Addgene also feels it’s incredibly important for scientific...distribution and disseminate knowledge around the globe. Addgene’s progress in international distribution As you ...are making great progress toward spreading the Addgene community into developing regions like South America...the countries that have requested plasmids from Addgene since our inception in 2004. In the near future...SeedingLabs or on Facebook Additional Resources on the Addgene Blog Learn About Our Recent Visit to China Read...People at Scientific Conferences Resources on Addgene.org Read about Global Plasmid Distribution Check... Institutions that Deposit their Plasmids with Addgene ...

...qPCR is used in a variety of applications including gene expression profiling, studying copy number variation... is also used in high throughput SNP genotyping, gene deletion analysis, and RNA detection.  A summary...starting template DNA concentration Gene expression profiling Copy number variations Quantify RNA levels...Disease or pathogen identification SNP genotyping Gene deletion Analysis RNA detection     References...in colony PCR, or for generating mutated sequences for site-directed mutagenesis.  Quantitative polymerase...101: Colony PCR Site Directed Mutagenesis by PCR Resources on Addgene.org Protocol: Polymerase Chain ...concentration of the original template DNA in the sample.  Addgene currently uses ddPCR for AAV titrating. You can...

...contain a toxin gene (regulated by a rhamnose-inducible promoter) and a chromoprotein gene. Allelic exchange...the plate. For example, if your plasmid contains a gene encoding a blue chromoprotein, after transforming...3: pTOX plasmids expressing amilCP and tsPurple genes. Image: Lazarus et al., 2019. One such lab is...protein family. What sets them apart is the way they generate their color: they strongly absorb visible light...difficult to directly use in bacteria, where most genetic manipulations start. Anthony Forster’s lab used...recombination events: The plasmid carrying the desired genetic modification integrates into the genome (Figure...art, let us know! Additional resources on the Addgene blog Find our Fluorescent Protein 101 blog series...

... the world, it can be hard to find your favorite gene, promoter, serotype, or a myriad of other things...Retroviral) Species: Specify the source organism for a gene insert. (e.g. S. cerevisiae) Plasmid Type: Choose...to find plasmids matching your search terms with gene and guide RNA / shRNA inserts Resistance Marker:...Resistance: Use this to choose the antibiotic resistance gene(s) used for selection in bacteria. Has Viral Service...terms in an Addgene plasmid search and found the results confusing, I’m sure you’ll find Addgene’s newly launched...Additional resources on the Addgene blog Learn more about other features on Addgene's website ...search, we’ve also revamped our homepage and mascot Blugene (check back on December 5 to learn more about this...

...including: generating single or multi-gene knockouts in a wide variety of cell types, gene editing using...efficiency, requires specialized equipment, such as a gene gun. Depending on the nature of the experiment, ... CRISPR-Cas9 Endonuclease for Cell-Type-Specific Gene Editing." Journal of the American Chemical Society...using homology directed repair (HDR), and generating large genomic deletions. What are the advantages of...and specificity is a concern, such as knockout generation or homologous recombination.  However, experiments...step in this type of CRISPR experiment is the generation of the RNP complex.  While one option is to purchase...in vitro transcribed from ssDNA, which can be generated by commercial vendors such as IDT. These two components...

...overexpress genes of interest; more recently we have also been using Addgene CRISPR vectors to generate cell ...cell lines with knockouts of specific genes. Many years ago, people in the laboratory became frustrated with...cDNA is co-transfected at a favorable ratio to the gene of interest (or, if your protein is fused with a...with different levels of expression of our gene of interest. Advantages of the agarose cloning method ...figure 1.   Find Additional Useful Protocols on Addgene's Protocol Pages Agarose cloning method procedure...-well cell plate to a 96-well assay plate. We generally freeze at least 2 ampules each of 4 clones with...som.umaryland.edu.   Additional Resources on the Addgene Blog Tips for a 1st Time CRISPR User (by a 1st...

....1216880109 Resources on Addgene.org CRISPR Plasmids – Protein Tagging CRISPaint Gene Tagging Plasmids 101...Resources on the Addgene blog Plasmids 101: Protein Tags Plasmids for Endogenous Gene Tagging in Human...kDas up to ~35 kDa and are typically knocked-in via gene targeting or cloned in-frame to the protein of interest... protease is situated between the degron and the gene of interest and the degron domain is terminal. Between...sequence (7 kDa). This system also requires less genetic engineering than some of the other systems reviewed... leakiness associated with the system, but the general concept of the system has remained the same. An...