We narrowed to 630 results for: Son

1. Antibodies 101: Introduction to Gating in Flow Cytometry

   Type

   Blog Post

   Published

   April 4, 2024, 1:15 p.m.

   ...’s software in order to generate signals in a reasonable range. If colleagues in your group already have...interest can still be gated out later. Based on personal preference, some people like to make stricter ...

2. CRISPR 101: Homology Directed Repair

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   Blog Post

   Published

   Jan. 26, 2023, 2:15 p.m.

   ...Site-Specific Genome Alterations. Edited by Doudna J and Sontheimer EJ. Methods in Enzymology Vol. 546; (2015). PubMed...inactive CRISPR-Cas9 using asymmetric donor DNA. Richardson CD, et al. Nat Biotech. 34(3); (2016). PubMed...

3. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

   Type

   Blog Post

   Published

   Oct. 11, 2016, 2:30 p.m.

   ...is also being realized. There are a variety of reasons to consider using genome editing to change the ...yellow striate mosaic virus (Gao et al., 2019) or sonchus yellow net rhabdovirus (Ma et al., 2020) have already...

4. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

   Type

   Blog Post

   Published

   April 9, 2019, 1:13 p.m.

   ...When both orientations are available, a direct comparison of these fusion proteins may reveal which of ...Unbiased, random insertions can be generated with a transposon-based strategy (Sheridan, 2002). Avoiding protein...

5. The Fluorescent Vegetables in Aptamer Soup

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   Blog Post

   Published

   Jan. 29, 2019, 2:27 p.m.

   ...do aptamers! Corn was recently described in 2017 (Song et al., 2017). Corn naturally dimerizes, and this...PMID: 28747322. PubMed Central PMCID: PMC5602116. Song, Wenjiao, et al. "Imaging RNA polymerase III transcription...

6. Harnessing Bacterial Toxins for Allelic Exchange

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   Blog Post

   Published

   Aug. 15, 2019, 12:30 p.m.

   ...arguably the most widely and productively used  (Thomason et al., 2014). In recent years, CRISPR-Cas9 technology...reports 5 (2015): 15096. PubMed PMID: 28060411. Thomason, Lynn C., et al. "Recombineering: genetic engineering...

7. Multiple Plasmids at a Low Price: Kits from Addgene

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   Blog Post

   Published

   Sept. 14, 2016, 2:30 p.m.

   ...RNAs." PloS one 11.2 (2016): e0149483. Rogers, Jameson K., and George M. Church. "Genetically encoded ...-tagged human ORF clones to allow testing and comparison of different cell-free expression systems; the...

8. How to Negotiate a Successful Lab Start Up Budget

   Type

   Blog Post

   Published

   Sept. 1, 2015, 2:30 p.m.

   ...structure associated to these tools, skills, and personnel Define pricing according to all of the needed ...managers, and techs’ financial books.  To find personnel salary ranges, go to online job information sites...

9. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

   Type

   Blog Post

   Published

   April 24, 2015, 2:39 p.m.

   ...Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Nature Methods. 2011 Mar;8(3):253-9. doi: 10.1038...Hadjieconomou D, Rotkopf S, Alexandre C, Bell DM, Dickson BJ, Salecker I. Nature Methods. 2011 Mar;8(3):260...

10. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type

    Blog Post

    Published

    Jan. 16, 2025, 3:42 p.m.

    ...Sleeper, M. M., Sanmiguel, J., Wu, D., Gao, G., Wilson, J. M., & Sweeney, H. L. (2008). Adeno-Associated..., K. L., Ayasoufi, K., & Barry, M. A. (2019). Comparison of Gene Delivery to the Kidney by Adenovirus,...