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We narrowed to 898 results for: CAN

Showing: 721 - 740 of 898 results

  1. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...training in a globalized world where individuals can exchange ideas, network, collaborate, and innovate...of people hold a PhD, achieving our career goals can be a real challenge. On top of that, in the midst... (s)he feels truly understood and supported, and can more fully identify with and be inspired by the mentor...a mentor who has experienced them before and who can serve as a role model and advisor. For their own ...research at UK in the same field that I do: prostate cancer. She has guided me in my next steps. For example...that their knowledge and skills had improved significantly when compared to their pre-program self-assesment...improvements, not necessarily quantifiable, but always significant. Doubts, fears, insecurities… all shrink when...

  2. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...microscope is not the only way to visualize them. You can also “hear” them. Addgene depositor Dr. Mikhail Shapiro...amoebasisters.tumblr.com/post/139944465237/lactose-around-we-can-make-some-enzymes-for   The take home message was...listening to talks all day. But nope, not at the American Society for Microbiology 2018 Microbe meeting....

  3. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 1, 2018, 1:57 p.m.
    ...protein B; but proteins are social biomolecules. They can be part of multiple dynamic and overlapping complexes...is pulled down, typically with an antibody. This can artificially select against PPIs that aren't stable...protein fragmentation complementation assay that can be used to study binary protein interactions. However...generally only tells you if two proteins interact and cannot be used to identify interaction partners other ...protein-protein interactions, SplitBioID provides a candidate list of proteins that are part of the bait proteins...interactome. Further characterization of these candidates is critical to understanding how they interact...

  4. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ...all AAVs that reproducibly labeled retinal cells can be found here.  Applications for synthetic promoter...specific cell types, pairs of synthetic promoter can be used to restrict expression to a single cell type...Cre-mCherry and GFP.   Figure 2: Synthetic promoters can be paired for targeted expression. This same pairing...

  5. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...optimization of the exon-splitting site sequence can enhance the rate of trans-splicing (Lai et al., 2005...sequences and the homologous region of the two vectors can be from one of the transgene’s endogenous introns...2014). Drawbacks: The choice of homology sequence can influence the level of transgene expression, and ... capable of expressing a truncated protein. This can be avoided by excluding a stop codon from donor A..., was 81% of that from a single vector, but significantly greater than the expression from an overlapping... the first HDR event, then the second HDR event can’t occur. Lastly, depending on its design, donor A ...

  6. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...the human, pig, or zebrafish genome, this system can be used in these organisms. Read the paper in bioRxiv... but with the addition of 4-hydroxytamoxifen, it can move to the nucleus for activity. They also developed...for cancer research The Sidi Chen lab recently published two CRISPR knockout libraries for cancer research...inhibition library CRISPR knockout libraries for cancer research Creating knock-ins with GeneWeld vectors...

  7. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...requestors, and supported by our repository. You can enjoy these stories, and other 20th anniversary celebrations... our diverse community, which I truly believe we can only serve through active conversation and collaboration...

  8. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    Published
    July 21, 2015, 2:30 p.m.
    ...through Science Careers’ myIDP tool. Using myIDP, you can identify suitable careers based on your current interests...and skillset. With this information in hand, you can then formulate a plan to further develop your transferable...

  9. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...screens in cancer cell lines, looking to uncover genetic dependencies of individual tumors, which can lead ...prevent Cas9 from cutting, both of which can introduce significant bias into the results. But the reference...genome – even single mismatches within a CRISPR guide can prevent the guide from binding to the genome or prevent...major ancestry groups for 994 of the Cancer Dependency Map cancer cell lines (left). Heatmap indicating...methodology, they estimate that the African ancestry-associated cancer dependencies are undercounted by ...development, particularly in cancers that are more common in individuals of African descent.Correcting the ...Broad Institute of MIT and Harvard, Dana-Farber Cancer Institute, and Koch Institute, where an exploration...

  10. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...genome is majorly composed of three proteins that can be easily modified in order to facilitate its entry...vectors As compared to other viral vectors, AdVs can provide a large transgene packaging capacity. For... DNA Adenoviral CRISPR/Cas9 genome editing tools can also be used to deliver Cas9 and DNA templates in...used so far) or for cancer gene therapy such as nonalcoholic steatohepatitis cancer. In deficiencies of...particular cancer. That’s exactly what the lab of Andrea Ventura at the Memorial Sloan Kettering Cancer Center... 2012). These features make AdV an attractive candidate for in vivo delivery of advanced genome editing...Center did to create a model of Eml4–Alk-driven lung cancer (Maddalo et al., 2014). These tools are available...

  11. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...me pursue this, let me make a slight change so I can have flexibility and be up to date with the current... It is incredibly helpful to me to know that you can make big changes in your career path and still find...done that, my big takeaway has been the impact I can have on an organization beyond my job description...

  12. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    .... Plasmids with wild-type Cas13a (formerly C2c2) can mediate targeted RNA knockdown, which leads to unique...cells with CRISPR? I certainly didn’t, but now we can with Cas13b/ADAR constructs from the REPAIR (RNA ...

  13. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    ...interest. Auxotrophy Besides antibiotic selection, you can select for a successful uptake of your DNA of interest...contain constructs that have lost the sacB gene. SacB can be used similarly to CcdB. For example, in the gene...

  14. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...their own CRISPR arrays. On the other hand, viruses can evolve to evade CRISPR by mutating these sequences...target in order to cut DNA, whereas some mismatches can be tolerated outside this region. Conclusion Ready...

  15. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ...little-known disease. You can find more information about the disease on the Meothelioma Cancer Alliance Website...lymphoma research or the many different ways you can improve your heart health. These are amazing advances...tumor samples that they have used to identify candidate genes as drivers of peritoneal mesothelioma tumorigenesis... to knockout, knockdown, or overexpress these candidates in a panel of ~30 patient derived peritoneal ...These experiments will serve to validate these candidate genes and allow Nabavi and colleagues to prioritize...

  16. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ... genomic loci in live cells. While other systems can be used to label loci, such as fluorescence in situ...potential solution is SunTag, a synthetic scaffold that can be used to recruit multiple protein molecules (and...stimuli. It could also have major applications to cancer, for example, the visualization of chromosomal ...

  17. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ... conventional PCR. Biallelically disrupted cells can be easily identified. Even in the case of clones ...of many available strategies by which biologists can imagine, create, and examine novel genetic configurations...post was contributed by Daniel Bauer and Matthew Canver of Boston Children’s Hospital and Harvard Medical...deletions in mammalian cell lines. Below Bauer and Canver discuss the motivations behind this research.   ... more at his webpage, bauerlab.org.     Matthew Canver is an MD/PhD student at Harvard Medical School ...Science 2013 Oct 11;342(6155):253-257. PubMed. 3. Canver MC, Bauer DE, Dass A, Yien YY, Chung J, Masuda ...

  18. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...weak, government support is “feckless”.  So what can be done?  He charged the postdocs to “Grab ahold ...destinies” and also suggested some concrete actions that can be taken now to solve some of the more “tractable...Workforce, Metrics and Incentives and Funding.  I can’t wait to see what solutions these talented postdocs...science to be happy so the beginnings of solutions can’t come too soon.  Watch the Future of Research Website...

  19. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    Published
    Sept. 15, 2014, 6:05 p.m.
    ...no doubt that companies are swarming to see what can be done with this technology to help patients. Dr... me, I continue to be amazed at how cool biology can be and how much we have yet to learn. As Dr. Mello... cure sickle cell anemia in stem cells but this can’t be used in people until we learn more about safety...

  20. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...infection first of Cas9, then with the sgRNAs, and can result in a higher titer due to the smaller size ...application offer proof-of-principle that CRISPR-Cas9 can be used to target any sequence in the genome. Tips... identify genes essential for cell viability in cancer and pluripotent stem cells. In a melanoma model...in search of genes involved in resistance to the cancer drug vemurafenib. Enriched libraries for select...
Showing: 721 - 740 of 898 results