We narrowed to 856 results for: ELL
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TypeBlog PostPublishedMarch 26, 2019, 1:40 p.m.... can be used to visualize cells and to quantify targeting of particular cell types. For example, the mDlx...
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5 Uncommon Ways to Gain Career Skills During an Uncommon Time
TypeBlog PostPublishedOct. 20, 2020, 1:15 p.m.... or more “important”, they just need to know you well enough to ask good questions and hold you accountable... can share it with others and get their input as well. Giving a great video presentation is going to be... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog PostPublishedJan. 21, 2014, 3:08 p.m....industry?” Once academia was the clear answer with a well-defined path to professorship. But recently a downward... professions increases your chances of finding a well-suited job. EAs shouldn't be viewed as an unproductive... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog PostPublishedApril 28, 2021, 1:15 p.m....hard-to-read sentences. Grammarly Grammarly is a lot like spell check, but since it’s an internet browser plug-in...managers and productivity tools for grad school Hello PhD Podcast episode 15 "Simple tricks for time management... -
Celebrate National Book Month with Addgene
TypeBlog PostPublishedOct. 3, 2019, 12:47 p.m....Director of Quality Control Jonathan Strange & Mr. Norrell by Susanna Clarke "A quasi-historical/fantasy fiction...fantasy, pop-sci, true crime, and so on. We’ve read well-known classics, like Austen’s Pride and Prejudice... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog PostPublishedJan. 12, 2021, 2:15 p.m....again or set up a meeting. If you really got along well, suggest a coffee chat in the near future. Even ...you succeed in any way. Don’t believe people who tell you that thank you isn’t necessary. I once got a... -
Pushing the Limits of DNA Assembly
TypeBlog PostPublishedSept. 6, 2017, 1:01 p.m....skip purification steps and end-repair steps with well-designed fragment overlap sections, while still ...of NEBuilder HiFi DNA assembly method As with any well-executed cloning endeavor, NEBuilder requires upfront... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog PostPublishedJune 20, 2017, 2:30 p.m....measure the pH of our samples, we used a solution of cell culture medium, which is buffered and contains the... 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer P, Manning MC, Fitchmun MI. Protein... -
Addgene Depositors Get More Citations
TypeBlog PostPublishedApril 20, 2017, 2:30 p.m....Addgene deposits? Even more interestingly, could we tell if depositing a plasmid with Addgene causes a paper... most relevant to plasmids: Genetics & Heredity, Cell Biology, Biotechnology & Applied Microbiology, Biochemistry... -
Resume Writing for Non-academic Science Careers
TypeBlog PostPublishedDec. 18, 2014, 4:06 p.m....for the next 5 years. Does an academic career work well with your life and career plan? This might be a ... - includes videos on general resume writing, as well as additional videos with emphasis for graduate ... -
Finding and Joining Your Dream Lab
TypeBlog PostPublishedApril 8, 2014, 2:50 p.m....professors how they hire scientists for their labs. Tom Ellis selects new hires from a pool of CVs collected from... awhile. Acknowledgments Thank you to Dr. Tom Ellis (Imperial College London), Dr. Connie Cepko (Harvard... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog PostPublishedFeb. 28, 2023, 2:15 p.m....quantitatively evaluating antibody staining, or counting cells are now routine, open-source software projects that...portable device for studying plankton using flow cells, has become an established alternative to high-priced... -
Targeted Mutagenesis with EvolvR
TypeBlog PostPublishedFeb. 21, 2019, 1:15 p.m....EvolvR could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks by...researchers to trace the progeny of a single starting cell. Additionally, multiplexing of guides allows EvolvR... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog PostPublishedJan. 25, 2024, 2:15 p.m....requirements in regard to their sequence (NGG, etc.) as well as their orientation relative to the gRNA (5’ or...References References Adler, B., Trinidad, M., Bellieny-Rabelo, D., et al. (2023). CasPEDIA Database: ... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog PostPublishedOct. 19, 2023, 1:15 p.m....allow scientists to express a gene of interest in a cell or animal model. AAV plasmids contain several important...Integrity on rAAV8 Production Using the Baculovirus/Sf9 Cells System. Hum Gene Ther Methods, 28(5), 277–289. https... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog PostPublishedMarch 23, 2017, 2:30 p.m.... facet of a project this big. Maintaining our excellent service and accuracy involves caring and careful... it took a lot of work and dedication to do this well ... because it did. So finally, a big thank you ... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog PostPublishedJune 30, 2016, 2:30 p.m....methylation should be considered when digesting DNA. Why? Well, even though Dam methylation sites are not specifically...later be verified by Sanger sequencing. How can I tell if my enzyme will cut? Whether performing a digest... -
Transferable Skills Guide: Public Speaking
TypeBlog PostPublishedApril 20, 2023, 4 p.m.... a journal club to my molecular biology class. A Cell paper. With six figures. Averaging ten panels each...strategy that can help. Even something as simple as telling yourself, “I can stop and check my notes if I need... -
Antibodies 101: Fab Fragments
TypeBlog PostPublishedSept. 5, 2024, 1:15 p.m....are trying to block but your primary antibody as well. Next, Fab and Fab’ fragments are almost interchangeable...fragments for multiplexed super-resolution microscopy. Cell Rep Methods 2(10):100301. https://doi.org/10.1016... -
5 Steps to Writing Grant Proposals for Grad Students
TypeBlog PostPublishedFeb. 11, 2014, 12:13 p.m....Moreover, avoidance of ‘to be’ verbs creates strong, well-directed meaning and delivers strength to the sentence...convince the reviewers that the proposal is strong, well-thought out, and will be successful when funded....