We narrowed to 738 results for: HA

1. Thoughts on the Future of Research 2015 Boston Symposium

   Type

   Blog Post

   Published

   Nov. 25, 2015, 4 p.m.

   ...Academies define a postdoc as “An individual who has received a doctoral degree (or equivalent) and is...

2. How to Negotiate a Successful Lab Start Up Budget

   Type

   Blog Post

   Published

   Sept. 1, 2015, 2:30 p.m.

   ...means they don’t have the money. If the department has a strict budget, or a lack of resources, then ask...

3. Plasmids 101: E. coli Strains for Protein Expression

   Type

   Blog Post

   Published

   Feb. 10, 2015, 3:06 p.m.

   ... BL21 (DE3) pLysE* Chloramphenicol (pLysE) pLysE has higher T7 lysozyme expression than pLysS; expression...

4. CRISPR-mediated Plant Base Editors

   Type

   Blog Post

   Published

   Jan. 3, 2019, 1:35 p.m.

   ...editors (ABE) What if you have another gene Y, which has a defective allele Ym and you need to convert an ...

5. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

   Type

   Blog Post

   Published

   May 13, 2025, 1:15 p.m.

   ...available at Addgene: psPAX2-D64V. This construct has the D64V mutation, a single amino acid change (from...

6. Antibodies 101: Beyond Surface Labeling

   Type

   Blog Post

   Published

   June 27, 2024, 1:15 p.m.

   ...selecting the primary antibody host species, as it has to be different from the other antibodies in your...

7. Fluorescent Proteins 101: When GFP lets you down

   Type

   Blog Post

   Published

   Aug. 23, 2018, 12:05 p.m.

   ...deprotonated state (Tsien, 1998). The deprotonated state has an absorbance maximum around 488 nm and it emits ...

8. Transferable Skills Guide: Conflict Resolution

   Type

   Blog Post

   Published

   Jan. 4, 2022, 2:15 p.m.

   ...organization, because confirming that every person has read and understood every communication that gets...

9. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

   Type

   Blog Post

   Published

   March 1, 2016, 3:30 p.m.

   ...where the vector that you want to use is very large, has a high GC content, contains a lot of repeats--any...

10. Plasmids 101: Common Lab E. coli Strains

    Type

    Blog Post

    Published

    Nov. 7, 2014, 2:56 p.m.

    ...OpenWetWare's E.coli genotype resource. Additionally, NEB has a great list of genetic markers for your reference...

11. Finding Your Science Policy Path

    Type

    Blog Post

    Published

    Sept. 22, 2020, 1:15 p.m.

    ...ever thanks to the virtual world that the pandemic has foisted upon us. Action Items:  A simple place to...

12. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type

    Blog Post

    Published

    Jan. 26, 2016, 3:30 p.m.

    ...any immunogenicity of the AAV vector Once delivery has been optimized, it’s important to ascertain how long...

13. Antibodies 101: Introduction to Antibodies

    Type

    Blog Post

    Published

    Jan. 19, 2021, 2:15 p.m.

    ...methods. Each type of antibody and production strategy has advantages and disadvantages compared to each other...

14. Antibodies 101: Designing Your First Flow Panel

    Type

    Blog Post

    Published

    May 28, 2024, 1:45 p.m.

    ...few commonly used fluorophores. Each fluorophore has a defined emission maximum. Note, you can also use...

15. Live and Let Dye: Self-Labeling Protein Tags

    Type

    Blog Post

    Published

    April 16, 2024, 1:15 p.m.

    ... These tags most often consist of an enzyme that has been mutated to block its catalytic activity and ...

16. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type

    Blog Post

    Published

    April 25, 2017, 2:30 p.m.

    ...high contrast ratio usually indicates that a PA-FP has a low level of spontaneous fluorescence in the absence...

17. CRISPR 101: Multiplex Expression of gRNAs

    Type

    Blog Post

    Published

    Sept. 10, 2020, 11:45 a.m.

    ...multiple gRNAs on one plasmid. Sound good? Addgene has many tools to help you multiplex - we’ll use mammalian...

18. Troubleshooting and Optimizing a Western Blot

    Type

    Blog Post

    Published

    Sept. 17, 2024, 1:15 p.m.

    ...your gel or blot, Table 1 (at the end of the post) has a number of helpful suggestions.  Gels Gel issues...