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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 855 results for: PLE;

Showing: 721 - 740 of 855 results

  1. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...The technique relies on the basic ability of complementary basepairs adenine (A) and thymine (T) to hybridize...PCR product. If you need to decrease error rate, please use one of these methods to ensure your insert ...

  2. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...efficient in primary fibroblasts. iTOP also works in multiple types of primary mouse cells, including neuronal...and continued protein expression would require multiple transduction events. For these reasons, iTOP is...

  3. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ... out more than necessary for my interview. The simplest place I could think of to start was “Marketing...about marketing would be to start reading a broad simple overview, learn some jargon and key phrases and...

  4. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...point of reference, Addgene commonly prepares NGS samples using both pre- and post-amplification DNA and ...representation is most easily understood visually. As an example, the curves from our recent amplification of the...

  5. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...(by CRISPRainbow) to 3-fold with CRISPR-Sirius. Triple color detection With a third sgRNA containing alternating...Central PMCID: PMC4364232. Ma, Hanhui et al. “Multiplexed labeling of genomic loci with dCas9 and engineered...

  6. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...and transgene expression (Zhou et al. 2017). For example, mutations in the RBE site reduce binding with ...AAV2 ITRs can be cross-packaged with capsids from multiple AAV serotypes (Wilmott et al., 2019), and different...

  7. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...portion of different antibody isotypes. Binding to complement system proteins, which can initiate a cascade... next to a different constant domain gene. For example if a B cell switches to producing IgE antibodies...

  10. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...change to a slide, think about whether the same principle applies to other slides in your deck.  If someone...audience and ask for their feedback before you complete your work. If you’re giving a public talk, ask...

  11. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...inspires students and teachers to apply engineering principles to biological systems. Today, what started in... in March to exhibit projects at any stage of completion, from pitch to prototype. Last year’s BioBuilderClub...

  13. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...of 2016. The top viruses of 2016 were (drumroll please)... Top Lentivirus: lentiCas9-Blast Most of Addgene...double floxed human muscarinic receptor 4 (Gαi-coupled) DREADD fused to mCherry, all under the control...

  14. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ... suspected to interact, are added to a cellular sample. Both antibodies have a short antibody specific... observation of individual endogenous protein complexes in situ by proximity ligation." Nature methods...

  16. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...several different species are present. One common example is using pre-adsorbed secondary antibodies (which... other antibodies) in experiments that require multiple antibodies that each detect a different antigen...

  17. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...deletion in MYBPC3 donated skin, blood and semen samples. Ma et al. designed plasmids containing Cas9 and...Read about High Fidelity CRISPR Variants Target Multiple Genes at Once with CRISPR Resources on Addgene.org...

  18. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ... for photoswitchable kinases.  Below are a few examples demonstrated in Zhou et al. 1. Testing kinase ...Markus W. Covert. "High-Sensitivity Measurements of Multiple Kinase Activities in Live Single Cells." Cell ...

  20. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ... not all grant duties to trusted students. For example, if the proposal call aligns with your work, your...Below are the typical sections in a NIH RFA, for example, and tips to consider while writing each section...
Showing: 721 - 740 of 855 results