We narrowed to 861 results for: REN;
-
TypeBlog PostPublishedAug. 25, 2016, 2:30 p.m....fail you. The benefits of lab management software aren’t just limited to large volume facilities; it can...above, any one of these steps can fail but, in our current setup, there are no explicit instructions on what...
-
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog PostPublishedNov. 24, 2015, 3:30 p.m.... such that they activate gene expression from different promoters. Moving to an in vivo system, Tang et...eager to see how you use them in your research! References 1.Tang, Jonathan C.Y., et al. “A nanobody-based... -
Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m....same type, but are not cross-compatible. Using different lox site variants allows Cre to catalyze more ...cre-dependent techniques? Let us know in the comments! References 1. Bacteriophage P1 site-specific recombination... -
Viral Vectors 101: Virus Safety
TypeBlog PostPublishedJune 22, 2023, 1:15 p.m....dividing up the essential components of a virus into different plasmids. For a retroviral system this looks like... health and safety department if they are not currently provided. Eye protection or face shields are ... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog PostPublishedMarch 23, 2021, 1:15 p.m....-Wagner’s lab produce different levels of dCas9 expression and contain different antibiotic resistance... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog PostPublishedJuly 23, 2020, 1:20 p.m....more precise editing in the lab. References and resources References Dong D, Guo M, Wang S, Zhu Y, Wang... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog PostPublishedMay 7, 2020, 1:15 p.m....2020). The generated ABE8s needed to produce 3 concurrent base edits to withstand antibiotic selection....great addition to the base editing toolbox. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog PostPublishedOct. 6, 2020, 1:15 p.m....experiment. Good luck with your experiment! References Bikard D, Jiang W, Samai P, Hochschild A, Zhang...dCas9-CBP and the SAM system differ in target preference. Sci Rep 9: . https://doi.org/10.1038/s41598-... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog PostPublishedAug. 30, 2016, 2:30 p.m.... discrimination is especially important in the current outbreak because of the neurological symptoms associated...applications in health, energy, and sustainability. References 1. Pardee, Keith, et al. "Rapid, Low-Cost Detection... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog PostPublishedOct. 12, 2017, 2:26 p.m....will produce the same unique SapI 5’ overhang, different combinations of connector and tag donor plasmids...light on previously unknown protein functions. References Schwartz, Matthew L. and Eric M. Jorgensen. “... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m....immunoprecipitate the entire complex in three different cell lines. The Gräslund lab plans to continue...of indels to measure time. These plasmids with different promoters driving expression of Cas9 were used... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog PostPublishedFeb. 7, 2019, 2:16 p.m....science. It's open-source. You can see all the current code on GitHub, ask us questions about it, or send.... It builds on existing popular web services, currently Crispor and Crispresso, that adhere to the same... -
Plasmids 101: Multicistronic Vectors
TypeBlog PostPublishedSept. 9, 2014, 8:20 p.m.... new eBook! Our goal was to create a one-stop reference guide for plasmids. We've combined our Plasmids...solutions necessary for advancing the field. References: 1. Internal initiation of translation of eukaryotic... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m.... acidic, or synaptic vesicles). Some FPs have different ex/em spectra (e.g. mKeima) or change fluorescent...Fluorescent Protein (GFP) Three Review Articles on the Different Types of FPs: Stepanenko et. al. (2008) “Fluorescent... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog PostPublishedDec. 12, 2013, 3:35 p.m....functions, which we know and love. Boolean is in reference to George Boole who in 1854 was thinking about...tell us readers, what do you want to make? References: Jerome Bonnet et al. "Amplifying genetic logic... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog PostPublishedOct. 10, 2023, 1 p.m....decorative" title="Check icon" %} References and Resources References Hwang, J. M., Piccinini, T. E., Lammel... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog PostPublishedOct. 4, 2018, 12:44 p.m....human genome-wide CRISPRko (knock out), CRISPRi (interference), and CRISRPa (activation) libraries that do...conditions such as model systems and primary cells. References 1. Doench, John G., et al. "Rational design of... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....about science whenever he's not off the grid. References Bischof J, Duffraisse M, Furger E, Ajuria L, ...plasmids and resources Visit our Molecular Biology Reference ... -
Summer SciComm Series: Modes of Communication
TypeBlog PostPublishedJuly 1, 2022, 1:15 p.m....enjoy and that plays at least slightly to your strengths. Otherwise, you’re going to drop your project ...scientific training. Cartoons may be read as “for children” or may need text for audience interpretation.... -
Hot Plasmids Spring 2024
TypeBlog PostPublishedMay 23, 2024, 1:15 p.m....editing efficiencies in a number of cell types at different genomic loci — in some cases, over 20-fold higher...prime editor. B, Prime editing efficiencies at different loci in U2OS cells. Image adapted from Yan et ...