We narrowed to 833 results for: ins
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TypeBlog PostPublishedMay 23, 2019, 12:26 p.m....questions. Practice your presentation for both instances. If your poster material is nearing publication...Connect with people on LinkedIn or Twitter, for instance. If you’ve had a particularly meaningful conversation...
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Polymerase Chain Reaction Overview and Applications
TypeBlog PostPublishedAug. 10, 2021, 1:15 p.m....plasmid of interest, for detecting the presence of an insert or piece of DNA in a sample such as in colony PCR...into approximately 20,000 droplets. Each droplet contains the material required for PCR amplification. Following... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog PostPublishedOct. 10, 2023, 1 p.m....time to produce beta-lactamase prior to division, instead of immediately experiencing the effects of the ... capable of correctly folding disulfide bonded proteins in its cytoplasm. Microbial Cell Factories, 11... -
A Guide to Starting Your Own Journal Club
TypeBlog PostPublishedFeb. 6, 2020, 3 p.m....including casual request via email, in person, or instant messaging but we as scientists often prefer data...failing in many academic journal clubs where institutional inertia drives the meeting rather than interest... -
Summer SciComm Series: Modes of Communication
TypeBlog PostPublishedJuly 1, 2022, 1:15 p.m....information. Personally, I would much rather read the instructions than watch a how-to video. My dad, on the other...love it and others will avoid it like the plague. Instead of looking for The One Method to Rule Them All,... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog PostPublishedSept. 3, 2015, 2:30 p.m....Eleven years later, Addgene is a company of fifty instead of three, and we are sending hundreds of plasmid...yard this year, though she generously offered. Instead we picniced outside of our new office space on ... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog PostPublishedSept. 23, 2014, 3:05 p.m.... vector (deposited by David Root of the Broad Institute) has consistently been one of the repository’s...satisfaction of checking off each step as you painstakingly complete your experiment – one small victory... -
Negotiating Work and Life: How to Find the Joy
TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m....run and the long run and so will your work. Nobody wins, and you need to make a change. #2 Thou shalt sometimes...tasks – try to resist the impulse to control by insisting things always be done your way. Be patient with... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m....you can either order a DNA pooled library, which contains a collection of plasmids in a single tube, or ...lentiviral CRISPR libraries. Protocols from the Broad Institute- A variety of protocols and guides regarding pooled... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog PostPublishedApril 16, 2020, 1 p.m....Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F (2017) Nucleic acid detection with ...Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F (2018) Multiplexed and portable nucleic... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog PostPublishedJuly 2, 2019, 12:51 p.m....is not amenable to FACS. Thus, the researchers inserted human CD8, a gene that encodes a surface protein...yourself whether gene overexpression is necessary (instead of gene knockout or knockdown, for which there ... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog PostPublishedMay 2, 2019, 12:41 p.m....manipulated by injected RNA and can express fluorescent proteins. This lab has created an automated tank for feeding... fusion is such that the two plants share RNA, proteins, and DNA. Euprymna scolopes (Joshua Rosenthal... -
Lab to Office Culture Shock
TypeBlog PostPublishedFeb. 5, 2019, 1:04 p.m....for scientists making this transition. In this installment of the Addgene career guide, we’ll think about... Eric J. Perkins... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog PostPublishedSept. 17, 2019, 12:40 p.m....open new ways for a novel therapeutic approach against drug addiction. Anxiety tests Rodents have a natural...are ahead for behavioral neuroscience to reveal insights into the complex relationship between genes, neurons... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog PostPublishedOct. 1, 2015, 2:30 p.m....circuit is sufficient to induce reinforcement (instills a sense of “reward”), then the animal will repeatedly...derived from direct comparative analysis of microbial opsins." Nature methods 9.2 (2012): 159-172.5. Goshen ... -
Finding Your Science Policy Path
TypeBlog PostPublishedSept. 22, 2020, 1:15 p.m....the mission so much that they will often move mountains to give advice and guidance to those that ask....great sources of volunteer opportunities. NSPN maintains a great resource guide including conferences, ... -
New Coomassie Protocol Video
TypeBlog PostPublishedOct. 31, 2023, 1:15 p.m....protocol can easily be adapted for other types of proteins. If this video is helpful, you can find our... -
New Antibodies in the Lab Animation!
TypeBlog PostPublishedNov. 19, 2024, 2:15 p.m....the Lab" shows how antibodies are used to study proteins via ELISAs, western blots, immunoprecipitation... -
Addgene Launches Partnership with IPI
TypeBlog PostPublishedJune 13, 2023, 1:15 p.m....announce the launch of our partnership with the Institute for Protein Innovation (IPI). Together, we’ll ... -
Antibodies 101 Series on TikTok
TypeBlog PostPublishedMarch 19, 2024, 1:15 p.m....our videos will also be shared on our @addgene Instagram account, or you can watch the full animation on...