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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 871 results for: sta

Showing: 721 - 740 of 871 results

  2. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...can be ordered as oligonucleotides and should be stable in the cell. gDNA guides will also need to be transfected...-stranded breaks, with either blunt (Cas9) or staggered (Cpf1) cleavage. NgAgo takes a different approach...

  3. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...including the product origin; purity, mechanism, and stability; and sequence of biologic product, mode of administration...drug’s immunogenicity in-vitro helps researchers understand a drug’s safety and efficacy in vivo.  ADAs ...

  4. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...sequenced There are several human disease models established in yeast, including a widely used Parkinson’s...well as glucose or glycerol). K. phaffii is only distantly related to S. pombe and S. cerevisiae; evolutionarily...

  5. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...Now with the DAP array, it is possible to use the state-of-art base editor, prime editor, or any CRISPR-...CRISPR-based gene editors, for correcting mutations or installing functional genes to study and potentially treat...

  6. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...each week, and there’s advice on how to actually establish that schedule. There’s also tips for setting writing... writing goals, tracking progress, and starting a writing group. Loop Habit Tracker app for Android  This...

  7. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...distribution. You can also use these tips as a starting point when you need to amplify a library for your...Typically they’ll be in a file in FASTQ format, a standard format for storing NGS reads and their corresponding...

  9. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ... demand for the related plasmid. Under such circumstances, we would observe this same correlation, but...ssrn.com/author=1232466.     Neil Thompson is an Assistant Professor of Innovation and Strategy at the MIT...

  10. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...many cases the plasmids themselves are only the starting point for the production of viruses used to deliver...stocks. Our research scientists have provided their standard lentivirus production protocol here and you can...

  12. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG  Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N)  ...the different components of CRISPR, It's time to start thinking about how you can used them in your experiments...

  13. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...some promoter activity persisted in the inactive state. The dynamic range, defined as the difference between...components, removing inducible promoters that could crosstalk with other pathways. They also reduced system ...

  15. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ... are positive for your marker would be a good statistic to compare between samples. In cases where there... immunotherapies. She is currently a Research Assistant in Arlene Sharpe’s lab at Harvard Medical School...

  16. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...GCE4All, an NIH-funded center located at Oregon State University focused on turning GCE tools developed...in your experiments, GCE4All is a great place to start. They host monthly webinars, workshops, and a bulletin...

  17. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...may be thinking, well, these are mostly fairly standard molecular and cell biology techniques. What’s ...step can affect the optimization of another. For instance, a sample may need more or less cross-linking ...

  18. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ... and of our data in particular and our deep understanding of Addgene’s customer’s needs. Categories We...engine to be so specialized that it appears to “understand” biology.  We hope you will give our new search...

  19. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ... into the bacterial genome. REPLACR-mutagenesis starts out in familiar territory, but quickly diverges...recombination then joins the linear PCR products into stable circular plasmids - no nick repair is needed. Since...

  20. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...only 44% and 66% indel frequency with the use of standard single gRNA constructs. When PTG constructs with...<200 bp tRNA-gRNA takes up half the space of a standard pol III-gRNA, which will facilitate CRISPR/Cas9...
Showing: 721 - 740 of 871 results