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  TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m....aptamers and MS2-binding fluorescent proteins were the state of the art in RNA imaging in vivo. These new “Riboglow... the brightness. Arne Fabritius and colleagues started with the FP mNeptune684 (originally described by...
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  A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue LightTypeBlog PostPublishedJune 17, 2021, 1:17 p.m....about the paper recently published by her lab and Mustafa Khammash's lab at ETH Zürich. “Instead of a graphical... it!Disclaimer: I had to cut it very abruptly to stay within the time limit. pic.twitter.com/j6rhCCby2m...
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  Using Addgene's New Viral Service for Your ResearchTypeBlog PostPublishedNov. 17, 2016, 3:30 p.m....repository are used to produce CRISPR tools, we’re starting the Viral Service with lentiviral preps for the...transductions, and overviews for beginners just starting work with virus. You can find all of our viral...
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  Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?TypeBlog PostPublishedJune 9, 2016, 2:30 p.m....can be ordered as oligonucleotides and should be stable in the cell. gDNA guides will also need to be transfected...-stranded breaks, with either blunt (Cas9) or staggered (Cpf1) cleavage. NgAgo takes a different approach...
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  Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome LoopingTypeBlog PostPublishedSept. 3, 2019, 1:15 p.m....of studying the effects of endogenous looping on distal promoters and enhancers, or have attempted to engineer...these LADL Anchors to genomic targets, such as a distal promoter and an enhancer. What makes LADL unique...
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  New and Upcoming Viral Vectors - June 2019TypeBlog PostPublishedJune 18, 2019, 1:15 p.m....high contrast between its resting state and its calcium-bound state, which was achieved by lowering the...
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  Plasmids 101: NGS Quality Control for Pooled LibrariesTypeBlog PostPublishedOct. 26, 2017, 1:59 p.m....distribution. You can also use these tips as a starting point when you need to amplify a library for your...Typically they’ll be in a file in FASTQ format, a standard format for storing NGS reads and their corresponding...
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  Addgene Depositors Get More CitationsTypeBlog PostPublishedApril 20, 2017, 2:30 p.m.... demand for the related plasmid. Under such circumstances, we would observe this same correlation, but...ssrn.com/author=1232466. Neil Thompson is an Assistant Professor of Innovation and Strategy at the MIT...
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  Predicting Adverse Reactions to Monoclonal Antibody DrugsTypeBlog PostPublishedFeb. 13, 2024, 2:15 p.m....including the product origin; purity, mechanism, and stability; and sequence of biologic product, mode of administration...drug’s immunogenicity in-vitro helps researchers understand a drug’s safety and efficacy in vivo. ADAs ...
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  DAP arrays for multiplex gene editingTypeBlog PostPublishedJuly 7, 2022, 1:15 p.m....Now with the DAP array, it is possible to use the state-of-art base editor, prime editor, or any CRISPR-...CRISPR-based gene editors, for correcting mutations or installing functional genes to study and potentially treat...
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  Early Career Researcher Toolbox: Free Tools for Science WritingTypeBlog PostPublishedApril 28, 2021, 1:15 p.m....each week, and there’s advice on how to actually establish that schedule. There’s also tips for setting writing... writing goals, tracking progress, and starting a writing group. Loop Habit Tracker app for Android This...
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  K. phaffii: Rising to the OccasionTypeBlog PostPublishedApril 4, 2023, 1 p.m....sequenced There are several human disease models established in yeast, including a widely used Parkinson’s...well as glucose or glycerol). K. phaffii is only distantly related to S. pombe and S. cerevisiae; evolutionarily...
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  With Our New Viral Service, We're Taking CRISPR Further!TypeBlog PostPublishedDec. 6, 2016, 3:30 p.m....many cases the plasmids themselves are only the starting point for the production of viruses used to deliver...stocks. Our research scientists have provided their standard lentivirus production protocol here and you can...
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  Components of CRISPR/Cas9TypeBlog PostPublishedFeb. 2, 2016, 5 p.m....xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) ...the different components of CRISPR, It's time to start thinking about how you can used them in your experiments...
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  Synthetic Photobiology: Optogenetics for E. coliTypeBlog PostPublishedSept. 8, 2015, 2:30 p.m....some promoter activity persisted in the inactive state. The dynamic range, defined as the difference between...components, removing inducible promoters that could crosstalk with other pathways. They also reduced system ...
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  Management for Scientists: Delegating is KeyTypeBlog PostPublishedFeb. 20, 2015, 8:30 p.m....approachable mistakes will be made that could have been prevented. Make sure everyone understands that no ...
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  Behind the scenes: Addgene’s new search engine and moreTypeBlog PostPublishedDec. 12, 2019, 2:22 p.m.... and of our data in particular and our deep understanding of Addgene’s customer’s needs. Categories We...engine to be so specialized that it appears to “understand” biology. We hope you will give our new search...
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  GCE4All: Making Genetic Code Expansion AccessibleTypeBlog PostPublishedJune 15, 2023, 1:15 p.m....GCE4All, an NIH-funded center located at Oregon State University focused on turning GCE tools developed...in your experiments, GCE4All is a great place to start. They host monthly webinars, workshops, and a bulletin...
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  Antibodies 101: ChIPTypeBlog PostPublishedMarch 10, 2022, 3:25 p.m....may be thinking, well, these are mostly fairly standard molecular and cell biology techniques. What’s ...step can affect the optimization of another. For instance, a sample may need more or less cross-linking ...
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  Flow Cytometry Readouts: Yes, No, and Everything in BetweenTypeBlog PostPublishedAug. 20, 2024, 1:15 p.m.... are positive for your marker would be a good statistic to compare between samples. In cases where there... immunotherapies. She is currently a Research Assistant in Arlene Sharpe’s lab at Harvard Medical School...