We narrowed to 904 results for: CAN

...email to get information if you can help it. Observe as much as you can in person. Rotate in the lab, if...Connie Cepko to learn about her mentoring style. How can I find out if an advisor is a good mentor? You have...for scientists to change labs – do as much as you can to ensure a good fit in advance. When a lab is interviewing... – I am different.”  Unfortunately, a bad mentor can be bad news for anyone. Try to speak to them more...excellent mentors out there. With some good pre-work you can find a lab that will deserve your hard work and an...

...dual nickases to generate a knockout or edit. This can help reduce off-target activity. Deleting a large... multiple genes at once. Using multiplexed gRNAs can target multiple locations in the genome to modify...common question Addgene Senior Scientists receive is: can I express more than one gRNA from a single promoter...plasmid is available from the Bullock lab, and gRNAs can be inserted using Gibson Assembly or SLIC cloning...sequence, creating flanking overhangs. These overhangs can be customized to link together multiple fragments...IIS enzyme, the unique flanking overhang sequences can link together to allow for ordered assembly into ...Lentiviral Expression Systems (MuLE) Kit: This kit can be used to create lentiviral vectors expressing wt...

...cleavage, the fluorescence is no longer quenched and can be visually detected using lateral flow strips. Since...technique Sensitivity: Single molecule per milliliter can be detected – in the zeptomolar range Time: ~1 hour...Cleavage results in fluorescence and this approach can be incorporated into a lateral-flow readout slip ...Protospacer Adjacent Motif) for target cleavage. This test can be performed in two steps for challenging samples...the ssDNA-FQ reporter. The resulting fluorescence can be observed under either no excitation light, UV ...field-ready, Bluetooth-enabled PCR machines that can even be battery operated, made accessible by the ...sequences. Upon binding, changes in fluorescence can be visualised. Another approach was to apply the ...

...measured via the light produced. A similar approach can be used to analyze cell signaling pathways or miRNA...have other advantageous properties. This approach can improve live-cell imaging by avoiding autofluorescence...luciferase subunit to other proteins, researchers can test whether those proteins interact, since light...luciferase components together. A similar approach can test protein-protein interactions using BRET. A luciferase...Considerations for luciferase assays Luciferases can only produce light in the presence of a compatible...however, secreted luciferase or alternative substrates can permit measurements of luminescence from live cells...Luciferase variants with incompatible substrates can also allow multiplexing with experiments that supply...

...was successful in your organism or cell type? You can use DNA sequencing or other molecular cloning techniques...without any off target effects. However, these methods can be labor intensive and quite time consuming.   As...activity.  By using the RFP:GFP ratio, edited cells can be sorted from unedited cells. In an experiment that... once a mutant is identified, the PB transposase can easily and seamlessly remove the integrated GFP reporter...fluorescent protein (RFP), named GEMCherry2, that can rapidly and efficiently evaluate Cas9 cutting sites...genomic region inserted into the N-terminus of mCherry can be targeted by a sgRNA guided Cas9 causing a DSB ...depending on the nucleotides inserted or deleted, can result in an in-frame shift of mCherry and thus expression...

...But base editors aren’t perfect. They may be slow, can only target certain sites, or make only a subset ...with a faster deamination rate so that deamination can happen before Cas homologs let go. The Liu lab developed...editor, a functional gene III product is produced and can infect bacterial cells. If a phage does not deliver... The editing speed dictates which phage variants can propagate faster than the rate of dilution (passage...editor was named ABE8e. ABE8e is not only fast, it can also be multiplexed: when tasked to correct two point...mutations. Together, cytosine and adenine base editors can correct all four of these kinds of point mutations...of ABE8e to the base editing toolbox, scientists can now make these edits much more efficiently and in...

...GPCR signaling can also modify other properties such as endogenous gene expression, which can lead to changes...chemogenetic receptors hijack cell signaling pathways and can lead to such responses as decreased heart rate (Redfern... is important to consider that the same receptor can elicit different responses depending on what cell...manipulation are neurons where chemogenetic receptors can be used to alter excitability by lowering or raising...). While some scientists argue that these issues can be mitigated by using low doses and running appropriate...such as C21, CLZ, Perlapine, and Olanzapine, that can also activate muscarinic-derived DREADDs. A ligand... of off target effects. These synthetic agonists can penetrate the brain and some, such as varenicline...

...link genotype to phenotype that can be used with mutational scanning: ligand binding assays, phage display...saturation mutagenesis Several methods can be used to create mutational scanning libraries. While each of these...saturation mutagenesis as a quick and easy technique that can be used to generate complex libraries of mutant plasmids...thousands of protein variants can be tested in one experiment. There are many functional assays that link... choosing and validating a selection system. You can test the effect of multiple mutations in combination... bases (NNN) at any given codon site of interest can be cloned into an expression vector with Gibson assembly...mutagenesis FAQ What’s the maximum length of codons that can be mutated? 88 codons, or 264 basepairs, was the ...

...at Harvard University. There are tons of ways you can get involved in science communication. In this second...from SITN, we discuss some of the many things you can do start your own science communication student group... Pint series and organize evens where scientists can grab a beer and chat about their work at a local ...the News, and member of the SITN podcast team. You can follow Vini on twitter @vinidhra.     Amy Gilson ...News, and is a member of the SITN podcast team. You can follow Amy on twitter @aigilson.   Special thanks...

... happen before you even receive the product? How can we consider the environmental impact of those processes... waste directly into the oceans.As consumers, we can choose products that are better for the environment...sacrificing performance or quality. To do this, we can use the ACT label, which is an eco-nutrition label...sciences, some of these plastics are high grade and can be washed and reused. This might sound risky in terms... incentives (for example, by grant agencies), we can shift toward a more sustainable one. The math on ...