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Showing: 371 - 380 of 601 results

  1. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ... and serve as a type of positive control. In the case of an unfavorable donor-acceptor orientation limiting...molecules, detect cellular processes and signaling cascades, quantify mechanical tension (a molecular “spring...

  2. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ... process, following sequencing and assembly. In cases where the QC team suspects an assembly issue, they...and be made available for distribution. In these cases, the QC team leaves a note on the plasmid page to...

  3. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ... Let’s get started! Before you begin In the best case, you know ahead of time that you want to probe your... to lose by trying to strip and reprobe. In this case, we recommend using mild stripping solution (see...

  5. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...the paternal for Prader-Willi syndrome). In both cases, the other copy of the chromosome is normally imprinted... exact genes, being improperly silenced in both cases. In both diseases, genes from both copies of chromosome...

  6. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...large (1-10) and small (11) fragments. In several cases an FP(1-10) fragment may be compatible with multiple...Split-horseradish peroxidase (Alice Ting Lab 2016)  Split-Cas9 for inducible genome editing (Feng Zhang Lab 2015...

  7. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...conjugated substrate is added to the wells. In the case where the detection antibody is biotin-conjugated... protein within a mixed sample. However, in this case your protein of interest is pre-incubated with a...

  9. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...the heading "Addgene Partial NGS Result." In some cases, there will be more information about our sequencing..., Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

  10. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...your intellectual freedom and integrity. (Not the case, as I’ve discovered.) As someone who has now seen...academia, as many of us know, this is not always the case. There are “difficult personalities” in every work...
Showing: 371 - 380 of 601 results