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We narrowed to 790 results for: Cre

Showing: 371 - 380 of 790 results

  1. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...Cas9 should be aware of the potential hazards of creating “gene drives” capable of spreading through wild...does making transgenic organisms with Cas9 risk creating a gene drive? The simplest way to make a gene ...appropriate flanking homology into a germline cell can create an RNA-guided gene drive. In principle, this event...Consequently, the best way to avoid accidentally creating a gene drive is also the easiest: don't use a ...

  2. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...White House as a Champion of Change, a program created as part of President Barack Obama's Winning the...DNA. We thought these were so much fun and so incredibly useful that we wanted to make them free to use... the invention of genetic engineering. What's incredible is the bioeconomy is already pretty substantial...product – equal to mining. There is an absolutely incredible hidden story here. The parts we are releasing...

  3. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ... bound by QCascade (Fig. 1B). The process also creates a 5-bp target-site duplication (TSD), a hallmark...Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically or by PCR/sequencing ...doi.org/10.1073/pnas.1709035114 Rubin BE, Diamond S, Cress BF, Crits-Christoph A,  He C , Xu M, Zhou Z, Smock...science.abb2920 Vo PLH, Ronda C, Klompe SE, Chen EE, Acree C, Wang HH, Sternberg SH (2020) CRISPR RNA-guided...

  4. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...teamwork, communication, writing, management, and creativity; I have developed these skills through school...not just “do the job”. When they ask about your creativity, your potential manager wants to hear a specific... job market, it is the transferable skills that create a full package and add an extra shine to your work...resolution Organization Leadership Problem Solving Creativity Data analysis Listening Negotiation Career Planning...

  5. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...purpose of MXS chaining MXS-chaining was designed to create plasmids for fluorescence imaging or flow cytometry...cells. Four individual expression constructs were created, each containing a unique fluorescent protein with...) and the four constructs were then combined to create a single polycistronic 15 kb insert. The final ...construction of plasmids with many components. The creators of the MXS-chaining method have used the technique...

  6. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ...more about this!). I’ve assembled a series of screenshots to highlight changes to both the design and functionality...NeuroMab Collection for details). The following screenshots show the range of different terms we can use ...the primary investigator where the plasmid was created can narrow your search results to exactly what ...experiment, and want to see if someone has already created and deposited it with Addgene. Combining search...

  7. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...new inflorescences begin to grow. This will also increase the number of inflorescences overall, which may...them again.  This won’t hurt them, and it will increase the chances of tDNA integration. Selecting Arabidopsis... seeds too densely during selection: this will increase background growth of non-transformants and make...Gateway Binary Vectors: High-Performance Vectors for Creation of Fusion Constructs in Transgenic Analysis of...

  8. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...assembly decreases as the cloning complexity increases. The efficiency of recombination is increased with ... minimize false positive colonies. We routinely screen just two colonies for simple cloning procedures...

  9. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...written portion of the grant application. They have increased their demand for stronger budgets with clear budget..., the amount of grant funds available and the increasing regulatory process for grants has fostered the... your own Professional Development Strategy to increase your competencies and skills in the grants profession...Management and Evaluation and/or earning the GPC credential from the Grant Professional Certification Institute...

  10. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...other epitope tags – which when doubled or tripled increase the tag size quickly – modifying the length a ...tag is not enough. What if you need one tag to increase solubility and one tag for purification? Or you...don’t want more tag than protein), 2-3 tags is increasingly common. Tandem affinity purification (TAP) once...protease itself, with various mutations used to increase its stability activity, can be readily purified...
Showing: 371 - 380 of 790 results