We narrowed to 932 results for: Gene

...    This is just the first of many milestones Addgene is poised to reach in the near future! We’re almost...April 8, 2021) Find these stats and more on the addgene.org homepage! You can track the number of plasmids...

...academia and industry. As a result over 90% of Addgene’s COVID-19 resources are now available to scientists...these groups were willing and able to listen to Addgene’s new approach of working with the industry-based...in the background became a normal occurrence as Addgene and our university partners quickly assessed how...allowed them to synthesize new plasmids quickly and Addgene’s quality control and archiving system allowed us...COVID-19 plasmid collection Beyond COVID-19: Addgene’s plasmid collections available to industry scientists... this alone. If you have deposited plasmids at Addgene and would like to make them available to industry...industry, please reach out at techtransfer@addgene.org and we will initiate a conversation with you and your...

...resources on the Addgene blog Learn more about about Addgene in our "Inside Addgene" blog series Subscribe...Subscribe to the Addgene blog Resources on Addgene.org Meet the other members of Addgene's Board of Directors... did you first hear about Addgene? How did you become involved with Addgene? Katz: Joanne and I first ...connection that got to know more about Addgene and heard that Addgene was looking for new board members. ... that Addgene provides the scientific community? Katz: The most important resource that Addgene provides...What do you hope Addgene will accomplish in the near future? Katz: I’m excited that Addgene is continuing... through Addgene’s sharing platform. Expanding further beyond the US and increasing Addgene’s international...

...Instrumental Access participants. Like Seeding Labs, Addgene also feels it’s incredibly important for scientific...distribution and disseminate knowledge around the globe. Addgene’s progress in international distribution As you ...are making great progress toward spreading the Addgene community into developing regions like South America...the countries that have requested plasmids from Addgene since our inception in 2004. In the near future...SeedingLabs or on Facebook Additional Resources on the Addgene Blog Learn About Our Recent Visit to China Read...People at Scientific Conferences Resources on Addgene.org Read about Global Plasmid Distribution Check... Institutions that Deposit their Plasmids with Addgene ...

... the world, it can be hard to find your favorite gene, promoter, serotype, or a myriad of other things...Retroviral) Species: Specify the source organism for a gene insert. (e.g. S. cerevisiae) Plasmid Type: Choose...to find plasmids matching your search terms with gene and guide RNA / shRNA inserts Resistance Marker:...Resistance: Use this to choose the antibiotic resistance gene(s) used for selection in bacteria. Has Viral Service...terms in an Addgene plasmid search and found the results confusing, I’m sure you’ll find Addgene’s newly launched...Additional resources on the Addgene blog Learn more about other features on Addgene's website ...search, we’ve also revamped our homepage and mascot Blugene (check back on December 5 to learn more about this...

...qPCR is used in a variety of applications including gene expression profiling, studying copy number variation... is also used in high throughput SNP genotyping, gene deletion analysis, and RNA detection.  A summary...starting template DNA concentration Gene expression profiling Copy number variations Quantify RNA levels...Disease or pathogen identification SNP genotyping Gene deletion Analysis RNA detection     References...in colony PCR, or for generating mutated sequences for site-directed mutagenesis.  Quantitative polymerase...101: Colony PCR Site Directed Mutagenesis by PCR Resources on Addgene.org Protocol: Polymerase Chain ...concentration of the original template DNA in the sample.  Addgene currently uses ddPCR for AAV titrating. You can...

...contain a toxin gene (regulated by a rhamnose-inducible promoter) and a chromoprotein gene. Allelic exchange...the plate. For example, if your plasmid contains a gene encoding a blue chromoprotein, after transforming...3: pTOX plasmids expressing amilCP and tsPurple genes. Image: Lazarus et al., 2019. One such lab is...protein family. What sets them apart is the way they generate their color: they strongly absorb visible light...difficult to directly use in bacteria, where most genetic manipulations start. Anthony Forster’s lab used...recombination events: The plasmid carrying the desired genetic modification integrates into the genome (Figure...art, let us know! Additional resources on the Addgene blog Find our Fluorescent Protein 101 blog series...

....1216880109  Resources on Addgene.org CRISPR Plasmids – Protein Tagging CRISPaint Gene Tagging Plasmids 101...Resources on the Addgene blog Plasmids 101: Protein Tags Plasmids for Endogenous Gene Tagging in Human...kDa up to ~35 kDa and are typically knocked-in via gene targeting or cloned in-frame to the protein of interest... protease is situated between the degron and the gene of interest and the degron domain is terminal. Between...sequence (7 kDa). This system also requires less genetic engineering than some of the other systems reviewed... leakiness associated with the system, but the general concept of the system has remained the same. An...

...including: generating single or multi-gene knockouts in a wide variety of cell types, gene editing using...efficiency, requires specialized equipment, such as a gene gun. Depending on the nature of the experiment, ... CRISPR-Cas9 Endonuclease for Cell-Type-Specific Gene Editing." Journal of the American Chemical Society...using homology directed repair (HDR), and generating large genomic deletions. What are the advantages of...and specificity is a concern, such as knockout generation or homologous recombination.  However, experiments...step in this type of CRISPR experiment is the generation of the RNP complex.  While one option is to purchase...in vitro transcribed from ssDNA, which can be generated by commercial vendors such as IDT. These two components...

...overexpress genes of interest; more recently we have also been using Addgene CRISPR vectors to generate cell ...cell lines with knockouts of specific genes. Many years ago, people in the laboratory became frustrated with...cDNA is co-transfected at a favorable ratio to the gene of interest (or, if your protein is fused with a...with different levels of expression of our gene of interest. Advantages of the agarose cloning method ...figure 1.   Find Additional Useful Protocols on Addgene's Protocol Pages Agarose cloning method procedure...-well cell plate to a 96-well assay plate. We generally freeze at least 2 ampules each of 4 clones with...som.umaryland.edu.   Additional Resources on the Addgene Blog Tips for a 1st Time CRISPR User (by a 1st...