...expression of a gene. Screen: A library of gRNAs with a CRISPR nuclease target multiple genes in a cell population...’re bringing you a brand new third edition of Addgene’s CRISPR 101 eBook. This new edition of the eBook...CRISPR nuclease. Note: Most CRISPR plasmids from Addgene produce either a gRNA, a nuclease, or both. Applications...

...experiment? What if after receiving the plasmid from Addgene you could directly start your digest, PCR, or transformation...preparation is quality controlled through next generation sequencing and spectrophotometry to test purity...Suggestion Form.  Additional resources on the Addgene blog Browse blog posts from our Plasmids 101 blog...the various ways to quantify DNA Resources on Addgene.org Learn about our other beta projects Read our...

...opportunity. That's how Addgene was founded. That's how my position and my team at Addgene was created.”- Eric...discussed on the Addgene blog, consider writing a guest post. Additional resources on the Addgene blog Read...transferable skills guide Resources on Addgene.org Find job listings at Addgene Get more career insight from Addgenies...  We touch upon these topics frequently in the Addgene blog. In fact, we have so many blog posts about...into one comprehensive, downloadable guide: the Addgene Science Career Guide. We also asked Addgenies what...

... to the Hot Plasmids episode! Expanding the optogenetics toolbox with CRY2clust Article contributed by...Basic Science (IBS) has developed another useful optogenetic tool that enables robust and efficient oligomerization...CRY2clust, when integrated into a Ca2+ -modulating optogenetic system (OptoSTIM1), caused two-fold faster changes...soCoChR: A novel opsin that enables single-cell optogenetics Article contributed by Meghan Rego  Listen ...Listen to the soCoChR podcast segment Optogenetic stimulation is a powerful tool used by neuroscientists to...promoters and/or fluorescent tags are available at Addgene. Shemesh OA, et. al. Nature Neuroscience. 2017...The design use to detect PKA activity can be generalized to measure phosphatase activity, second messenger...

...the antibiotic resistance gene and origin will replicate, and the transgene will be lost. Over time, bacteria...vector backbone contains the antibiotic resistance gene (AmpR) and origins of replication (ori and f1 ori...these cloning strains, browse the table of common gene mutations in our Plasmids 101: Common Lab E. coli...Working on the scientific support team at Addgene, I get to see everything that can go wrong with a plasmid...recombination, one resulting plasmid carries the transgene of interest (aka the viral genome) and the other...10.1186/s12934-020-01321-4   Kolodner R (1980) Genetic recombination of bacterial plasmid DNA: electron...10.1261/rna.069286.118 Additional resources on the Addgene blog Find more molecular biology and viral vector...

...2018), and hybridomas can lose gene expression of the mAb-encoding genes, die, or fail to revive after ...broadly, advances in gene synthesis, affinity reagent screening, and next-generation sequencing of hybridomas...grants and Addgene’s mission This is the first NIH grant that Addgene has received. Addgene prides itself...allow Addgene to further our mission of scientific sharing,” says Dr. Daniela Bourges, Addgene’s interim...opened the door for a new generation of recombinant affinity reagents. Addgene is looking forward to working...antibodies Resources on Addgene.org Find recombinant antibody plasmids at Addgene ...to improve upon these critical research tools. Addgene is excited to announce that we have recently received...

...targeting a gene associated with diphtheria toxin lethality and were sorted into single wells to generate clonal...instance, it’s much more difficult to introduce a gene of interest into primary cells, so most primary ... risk of viral integration causing mutations and gene expression changes elsewhere in the genome. Second...j.cell.2015.03.028. PubMed. Find the Plasmids @Addgene: iTOP-tested proteins and reporters Previous...

...simultaneous activation and/or repression of multiple genes, and more. The researchers now plan to adapt the...powerful tools to modify the expression of multiple genes at the same time.” For more details on how to apply...the Mulitplex CRISPR/Cas9 Assembly System Kit at Addgene here. Other Popular CRISPR Posts: How to Design...

...protein coding genes in E. coli and S. cerevisiae expression vectors are available at Addgene. A set of mammalian...yeast expression constructs contain protein coding genes, tags, truncations, and codon optimizations similar...of plasmids containing SARS-CoV-2 protein coding genes in E. coli and S. cerevisiae expression vectors ... available at Addgene Read about SARS-CoV-2 detection methods Resources on Addgene.org Find all plasmids...been functionally tested by Ginkgo Bioworks or Addgene. If you used plasmids from this collection in your...COVID-19 Plasmid Collection Quality control at Addgene in the midst of COVID-19 We received Ginkgo’s first... our standard full plasmid sequencing by NGS. Addgene’s QC scientists immediately screen these plasmids...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional... Plasmids podcast series, you'll learn about optogenetics tools for controlling protein activity, methods...Base Editors Hot Plasmid 4: pPSU Plasmids for generating DNA molecular weight markers.  Listen to the ...Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie Cepko...Science Videos from Tom McFadden Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ... optogenetics...