We narrowed to 888 results for: URE

...experiments; large amounts of virus are required to ensure that you get full library coverage in pooled library...and genome-wide libraries for CRISPR screening." Nature methods 11.8 (2014): 783-784. PubMed PMID: 25075903...

...simpler and maybe smaller Cas systems could exist in nature. To do this, they mined a database of microbial...Lucas B., et al. "Programmed DNA destruction by miniature CRISPR-Cas14 enzymes." Science 362.6416 (2018)...

... RNA binding domains depends on the secondary structure of RNA. As a result, targeting the RNA of interest...better understanding of the phenomenon soon.  Rest assured, as the PUF protein design tools continue to evolve...

...sharing research materials — we're accelerating the future of science. Want to learn more about our latest...consensus platform for antibody characterization. Nature Protocols. https://doi.org/10.1038/s41596-024-01095...

... so be sure to check back often! To find more viral vectors available through Addgene, be sure to check...

...started making regulations such as preventing manufacturers from dumping hazardous waste directly into the...levels. Disposables are an accepted part of lab culture, but with the right incentives (for example, by...

...King soundtrack and writing this post, it is my pleasure to announce that we once again reached new heights... remember the hazards of that chemical in your obscure growth medium, these apps can help make your lab...

...pooled format at a more-typical scale of tissue culture while still offering the potential for true biological...and minimize off-target effects of CRISPR-Cas9." Nature biotechnology (2016). PubMed PMID: 26780180. PubMed...

...Rather, they relied on published studies in the literature to curate the perfect library, obtained the appropriate...It could [be used to screen for] morphological features, migration, or survival in different contexts....

...focus wholly on the science I love. This immersive nature of academia often means that scientists-in-training... video call. If you do decide to use Skype, make sure to follow the rules of successful, remote conversations...

...agencies with over $100 million in annual R&D expenditures to support increased public access to the results... guided by aspirations to promote open science, ensure good stewardship of clinical trial data, serve ...

...based on how each one impacted cell viability (see figure). Most of the mutations were found to be “neutral... gene aberration drivers of pancreatic cancer." Nature communications 7 (2016): 10500. PubMed PMID: 26806015...

... can’t find the molecular cloning guide you were sure was in one of those four white binders. Thirty minutes...) and place them on an easy to reach shelf. Make sure all notebooks are visibly labeled on the spine. ...

...Plasticity in Mentoring" A recent post on the blog “Tenure, She Wrote” is titled, “What Kind of Mentor Do ...answers to the other six mentoring questions in future posts. If you have a question about mentoring that...

...targeted with optical stimuli. The restricted light exposure to a specific area in combination with delivery...information from a region. In the example shown in Figure 1, injected retrograde AAVs at one location result...

...complemented each other's strengths: Ginkgo Bioworks’ infrastructure allowed them to synthesize new plasmids quickly...enables science to determine the three dimensional structures of proteins.  The RESOLUTE Consortium collection...

...need to be able to replicate in Agrobacterium to ensure that they are maintained during co-cultivation ...plasmid can also be customized due to its modular structure. Two multiple cloning sites (MCS's) flank the ...

...answer to that question. Additionally, a concurrent Nature Biotechnology article describes the development...Tan E-P, Del Castillo Velasco-Herrera M, Yusa K. Nature Biotechnology. 2013 Dec 23. (Article). Read more...

... a closer look at the rep and cap genes. Figure 1. The RepCap plasmid and its products. Created...265 . https://doi.org/10.1016/j.omtm.2019.01.008  Maurer AC, Weitzman MD (2020) Adeno-Associated Virus Genome...

...Publication policy with two changes that will help us ensure that plasmids don’t get left behind in this status...Addgene! We'll continue to review our policies to make sure they meet our goals, maintain your trust, and meet...