We narrowed to 908 results for: ANT

...fluorescent protein control. We also added the toxin-antitoxin stability cassette Axe-Txe so that the plasmid...plasmid could be maintained in the absence of antibiotics, resulting in the plasmid pBAD-bARGSer-AxeTxe (Fig...agents to boost expression to appreciable levels. We wanted to create second-generation mARGs with higher expression...

...fragments are likely non-functional, they could be dominant-negative and/or toxic. A potential solution is..., was 81% of that from a single vector, but significantly greater than the expression from an overlapping...While the hybrid dual vector system has a slight advantage over the overlapping and trans-splicing approaches...

...photo-stimulated through fiberoptic cannulas directly implanted in the injection site and connected to a laser...Taylor et al., 2016; Hooper and Maguire, 2016). Recombinant adeno-associated viruses (rAAVs) have become ... The resulting fusion gene is inserted in the antisense orientation with respect to the promoter to prevent...

...your data by Nov. 12 to compete. The top ten participants will be invited to compete in our speed round...winner each receiving a $500 travel award. All participants will receive an Addgene t-shirt.  Our judges...

... suggestions in the comments.  X has moved significantly away from the functionality and features that...this reason, our timeline has accelerated. We significantly reduced posting on November 20, 2023, and as...

...more commonly known as fruit flies, have been an important animal model for research, particularly genetics...well, annoy. Fly-related Issues in the Lab A significant drawback of working with fruit flies is their...

...tube-labeling, quick plate pouring, and much more. We didn’t want to keep the knowledge we’ve gained along the way...trick When combining reagents in the lab, it is important to mix them thoroughly before use.  For efficient...

...worth of ladder. The Penn State ladders are significantly cheaper. When using a homemade plasmid prep ...increases the costs to $20-30 per ladder: still significantly cheaper than commercial ladders. Are you interested...

...ventilation, and lab lighting/HVAC requires a significant energy expenditure here at Addgene. These are... critical systems that cannot be removed or substantially reduced in an attempt to save energy. The transition...

...story of Rosalind Franklin and her incredibly important role in solving the structure of DNA. 2. The...her or her family's knowledge. 3. Fossil Rock Anthem One of Tom's earliest and most popular videos. ...

...biologist Tips for protein purification Replica plating Anti-CRISPR Retroviral vectors Career development for...for the Addgene blog simply email us. We can’t guarantee that all those who sign up will be able to write...

...that you can stage in a lab diorama. Or maybe you want to create a stack of wrapped gifts using life science... festivities - however you celebrate! And if you want to see how Addgene celebrates and decorates, follow...

... focusing on safety and the ability to generate mutant microbial strains faster than ever before. 29:54... end : How Alex hopes to help Addgene advance - plants for trimmed down protocols, distribution of new...

...References 1. Choi, Vivian W., et al. "Production of Recombinant Adeno‐Associated Viral Vectors for In Vitro and...18265393. Additional Resources on the Addgene Blog Important Considerations When Using AAVs AAVs Are Great ...

...in CRISPR including: Base editing Prime editing Anti-CRISPRs … you name it! So get excited and download...multiple genes in a cell population. The resulting mutant cells are screened for phenotypes of interest. ...

...  has seen a fantastic response from the research community. Customers have taken advantage of the customizability...

...search bar Now, you can search for the articles you want to find without even leaving the Addgene blog! Just...articles on the Addgene blog by subscribing to instant alerts below.  ...

...toiling away in laboratories this holiday season want the work you are doing to have an impact, to move...libraries and offering another way to consider and quantify the impact of scientific publications. Ventures...

...expression level without the need for a custom antibody. There is one major caveat - a tag may interfere...affinity for other proteins, and accessibility to an antibody. From their initial pool of candidates, they selected...

...week. So I have developed some good habits that I wanted to pass on to you to make sure you are getting ... you properly visualize your results.The most important of those is to always look closely at the trace...