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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 890 results for: ANT;

Showing: 741 - 760 of 890 results

  1. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    Published
    Dec. 5, 2014, 2:54 p.m.
    ...toiling away in laboratories this holiday season want the work you are doing to have an impact, to move...libraries and offering another way to consider and quantify the impact of scientific publications. Ventures...

  2. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ...expression level without the need for a custom antibody. There is one major caveat - a tag may interfere...affinity for other proteins, and accessibility to an antibody. From their initial pool of candidates, they selected...

  3. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ...week. So I have developed some good habits that I wanted to pass on to you to make sure you are getting ... you properly visualize your results.The most important of those is to always look closely at the trace...

  4. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...bioluminescent, some types of corals and anemones are vibrantly colored. He hypothesized that GFP-like proteins...they’re used, see some new techniques that take advantage of them, and give some tips on deciding which ...

  6. Developing Transferable Skills During Science Training

    Type
    Blog Post
    Published
    June 3, 2014, 2:14 p.m.
    ...accomplishments. Employers and academic departments want to be able to tell that you know about something...Learned Sooner by Peggy Klaus Looking for a mentor or want to start a peer mentoring group? "What Makes a ...

  7. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ...choosing a specific serotype, but occasionally you may want to further control where your gene of interest is... many more tips and ideas on how to use these fantastic tools in your experiments.  Many thanks to Hongkui...

  8. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ... they’ve engineered SECURE adenine base editor variants using a protein-truncation strategy with reduced...Transient expression of CRISPR/Cas9 in cells has advantages like reduced off-target activity and decreased...

  9. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ...and see if we should start celebrating papers and grants too with pizza or pub.” The Cepko Lab celebrates...required to achieve success in the lab, it is important to take the occasional breather and acknowledge...

  10. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...Organize you experiments, keep track of upcoming grant deadlines, and sync everything to your calendar.... updates to protocols in real time. You may also want to consider moving your paper spreadsheets for reagent...

  11. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...double strand breaks. Researchers have long taken advantage of this natural process to integrate protein tags...selectable gene product for isolation of successful transformants. Addgene distributes several ready-to-use, modular...

  12. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...physical scientists are underrepresented. It’s important to note that these results could be biased for...Resources Check out the Top 50 Science Stars on Twitter Want suggestions on how to use social media as a scientist...

  16. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ... Society, or maybe you think about a local food pantry or community arts organization. Many people are...resources continuously raising money from donations or grants to fulfill their mission. Addgene is fortunate ...

  17. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...Experiments Experiment  Detection Method Notes Quantification of a process Plate-based assays (plate reader... HPLC (proteins or other molecules) Isolate or quantify cells, proteins or other molecules that have been...

  18. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ... et al calls this method acoustic collapse.  Importantly, acoustic collapse doesn’t affect bacterial viability... of ARG constructs with new acoustic phenotypes. Want to start using acoustic reporters for your research...

  19. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...different cellular responses. One of the biggest advantages of such a tool is being able to identify specific...screening technique. Lastly, the activity of both antagonists and inverse-agonists could be measured using ...

  20. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...two inverted terminal repeats (ITRs); but when recombinant AAV (rAAV) is made in the lab, rep and cap are...2020) Adeno-Associated Virus Genome Interactions Important for Vector Production and Transduction. Human ...
Showing: 741 - 760 of 890 results