We narrowed to 798 results for: Arc

1. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

   Type

   Blog Post

   Published

   Sept. 4, 2018, 12:53 p.m.

   ...Researchers express genes of interest from plasmids in order to study gene function or to engineer cells...

2. Giving gRNAs a Facelift - Synthetic and Beyond

   Type

   Blog Post

   Published

   Oct. 20, 2022, 1:15 p.m.

   ...stable than most macromolecules used in scientific research such as DNA or protein. It comes as no surprise...

3. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   Published

   Dec. 14, 2021, 2:15 p.m.

   ...p300 or the DNA methyltransferase DNMT3A allows researchers to easily add biochemical marks to genetic regions...

4. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

   Type

   Blog Post

   Published

   Nov. 14, 2024, 2:15 p.m.

   ...Cas9 system in human cell lines. Nucleic Acids Research. 2014;43(3):e17. doi:10.1093/nar/gku1198...

5. Antibodies 101: Stripping and Reprobing Western Blots

   Type

   Blog Post

   Published

   Aug. 1, 2024, 1:15 p.m.

   ...reprobe your western blot several times — some researchers report up to ten cycles! Just remember that a...

6. Fluorescent Proteins 101: Introduction to FRET

   Type

   Blog Post

   Published

   June 27, 2017, 1:03 p.m.

   ... Before constructing your own FRET probe, try searching PubMed for articles describing the FRET tool that...

7. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

   Type

   Blog Post

   Published

   Aug. 20, 2019, 12:31 p.m.

   ... viral protocols and videos Find AAVs for your research ...

8. Antibodies 101: Choosing the Right Isotype

   Type

   Blog Post

   Published

   April 6, 2023, 3 p.m.

   ...After all, there’s no sense in spending valuable research dollars on a new secondary antibody if you don...

9. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 1, 2018, 1:57 p.m.

   ...half-lives. Interested in using Split-BioID with your research? Plasmids are available from Addgene! References...

10. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    Published

    March 7, 2024, 2:39 p.m.

    ...alleles, and the growing need for gene editing in research, the old PAM constraints just won’t cut it anymore...

11. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    Published

    Jan. 5, 2021, 2:15 p.m.

    ...fluorescence-activated cell sorting (FACS) helps researchers sift through thousands of cells to identify cells...

12. Plasmids 101: Terminators and PolyA signals

    Type

    Blog Post

    Published

    March 31, 2016, 2:30 p.m.

    ...expression in a context-dependent manner. Nucleic Acids Research. 2000. PubMed PMID:10648781. PubMed Central PMCID...

13. Tips for Titering Your Lentiviral Preps

    Type

    Blog Post

    Published

    March 15, 2016, 2:30 p.m.

    ...resistance-genes, or the transgene itself. Many researchers prefer to design primers that target the common...

14. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type

    Blog Post

    Published

    Jan. 21, 2015, 1:30 p.m.

    ... to Expedite Your Experiments and Achieve Your Research Goals    ...

15. Polymerase Chain Reaction Overview and Applications

    Type

    Blog Post

    Published

    Aug. 10, 2021, 1:15 p.m.

    ... was first identified in the late 1960s during research at hot springs in Yellowstone National Park.  ...

16. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    Published

    Dec. 10, 2020, 1 p.m.

    ...using adenoviral vectors with your CRISPR/Cas9 research! To find more information about the adenoviral...

17. Cloning Mammalian Cells with the Agarose Method

    Type

    Blog Post

    Published

    Sept. 7, 2017, 12:17 p.m.

    ... biological screens. For example, for cancer researchers, if growth properties in agarose are of interest...

18. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type

    Blog Post

    Published

    June 22, 2017, 2:30 p.m.

    ...function of a protein or its role in a disease, researchers often isolate proteins of interest and examine...

19. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type

    Blog Post

    Published

    April 4, 2019, 12:53 p.m.

    ...knockdown, however, keeps bacteria viable and allows researchers to study if that gene is a target of an antibiotic...

20. Viral Vectors 101: Voltage Indicators

    Type

    Blog Post

    Published

    Jan. 30, 2024, 2:15 p.m.

    ...ability to monitor their signals – electricity! The research tools for studying voltage within a cell are genetically...