We narrowed to 898 results for: CAN
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TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m....with a flexible linker where a protein of interest can be inserted. B) Protein expression and labeling procedure...voltage indicator (GEVI) than previous versions. ASAP5 can be imaged using one- or two-photon microscopy to ...Altogether, if you want a negative-going GEVI that can be used in different illumination regimes with fast...with non-neurodegenerative diseases (Diabetes & cancer). Image adapted from Wu and Wang 2024 under a CC-BY...
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Magnetic Control of Proteins: More than a Dream
TypeBlog PostPublishedSept. 24, 2024, 1:15 p.m....ve ever had an MRI, you know that magnetic fields can pass right through our tissues unimpeded. Andrew ...engineering approaches and that magnetosensitive tools can be developed from other fluorescent proteins. Currently...optogenetic tools, they’re optimistic that MagLOV can be similarly adaptable into future magnetogenetic...Maria’s screens of AsLOV2 variants showed some significant gains! After a few more rounds of mutagenesis...ideas and are happy to speculate, they know they can’t answer these questions alone. They readily admit...Confined Persistent Diradical Emitter. Journal of the American Chemical Society, 145(25), 13615–13622. https:... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog PostPublishedFeb. 9, 2023, 2:15 p.m....confidence and learn to work independently, which can lead to greater success in meeting educational goals...without challenges. Real research is messy, and it can be difficult to give up the control embedded in “... Course-based undergraduate research experiences can make scientific research more inclusive. CBE Life...the training of the mind to think something that cannot be learned from textbooks” (Brian, 1996). But is...citrate synthase and presented their findings at the American Society for Biochemistry and Molecular Biology... -
Experimenting with New Careers while in Grad School
TypeBlog PostPublishedFeb. 25, 2016, 3:30 p.m....science outreach, not only can I easily share science with the public, but I can watch and learn from the...dinosaurs every day. They made me happy. Grad school can provide plenty of negativity, so I volunteered at...wonderfully supportive mentor and graduate office. I cannot stress how important they were to my success as... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog PostPublishedAug. 5, 2014, 6:51 p.m.... data easily available, organized in a form that can be understood by others, balanced against how often...rarely preserve “raw” data of any sort in a form that can be parsed by someone other than the actual author...Addgene helps with DNA reagents, these resources can offload the burden of data storage and distribution... storing data in a sharable form is indeed a significant effort. Our standards of data recording are highly...Research Institute at Mount Sinai Hospital, Toronto, Canada where he studies protein-serine/threonine kinases... -
Beginner's Guide to Viral Vectors
TypeBlog PostPublishedJune 2, 2017, 2:30 p.m....You can use viral vectors for many experimental purposes. To help you make sense of all the viral vector... -
Meet the New Addgene Board Members!
TypeBlog PostPublishedApril 28, 2020, 1:14 p.m....age, I was fascinated by the impact that medicines can have on human health. As a teenager in high school...from for profit organizations are less common, they can be very impactful. At Novartis, Stegmeier deposited...high-resolution tracking of more than 1 million cancer cells under drug treatment.” Stegmeier especially... -
New and Upcoming Viral Vectors - December 2019
TypeBlog PostPublishedDec. 19, 2019, 2:40 p.m....AAV we have released in the last few months. You can also browse our entire AAV inventory. Our new AAVs...in target cells. While the various AAV serotypes can be used to achieve different patterns of expression... -
The Strength of Story Telling
TypeBlog PostPublishedDec. 19, 2017, 2 p.m....story short, telling a good story is something that can be learned, and will serve you well in your career... people enjoy and engage with and what parts you can leave out. Start viewing your work in the form of...other options. Choose something with emotional significance that is likely to be memorable. Work out what... -
The Golden Stopwatch Award: Recognizing Facilitators of Research
TypeBlog PostPublishedSept. 5, 2019, 1:06 p.m.... themselves from undue liability. Before Addgene can begin distributing a material, a deposit agreement...deposits with and/or requests materials from Addgene can qualify. We also reward improvement against one’s...Dana-Farber Cancer Institute Fred Hutchinson Cancer Research Center Memorial Sloan-Kettering Cancer Center... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog PostPublishedJuly 16, 2019, 12:59 p.m....shown that some channels, such as ChR2 and NpHR, can even be expressed simultaneously and still be individually.... Zebrafish behaviors Several behavioral assays can be done on zebrafish at 5 days post fertilization... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog PostPublishedMay 9, 2019, 1:15 p.m....or the clonal evolution of cancer. If you’re looking to use CellTag, you can find the CellTag libraries...progenitors (iEPs), a self-renewing cell type that can differentiate into hepatic or intestinal cell types... -
Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation
TypeBlog PostPublishedNov. 8, 2018, 3:47 p.m....Communications announcing a new bioinformatics tool that can predict whether a gene is natural or synthetic just...Classifying genes as natural or synthetic But how can you tell if a gene is synthetic or natural? The answer... -
Year of the Camelid: Antibody Style
TypeBlog PostPublishedMay 9, 2024, 1:15 p.m....for diverse applications. For example, RANbodies can be used as an alternative to conventional primary...target antigens in living cells or tissues. sdAbs can also be used to manipulate cellular functions, like...sdAbs have become appealing candidates for the detection of different cancers (Jin, et al. 2023). So where... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog PostPublishedDec. 12, 2023, 2:15 p.m....drugs targeting these receptors cannot be studied using GRABNE. They can, however, be studied with the ... antagonist. This is important because alpha2-AR can provide inhibitory feedback in response to norepinephrine...GRABNE, with both nLightG and nLightR showing significantly increased selectivity for norepinephrine over... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog PostPublishedJuly 27, 2021, 1:15 p.m....for enrichment of successfully targeted cells that can be used to generate organoid cultures with a modification...identified, functions at temperatures up to 43 °C and can be used for genome editing in moderate thermophiles... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog PostPublishedOct. 29, 2015, 2:30 p.m....research, they are now seen as an ubiquitous tool that can be diversely applied to many different experiments...Ed.) eBook And now for some exciting news... You can now download the newest version of Addgene's Plasmids... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog PostPublishedJan. 21, 2014, 3:08 p.m....to engage in extracurricular activities (EA). EAs can: Introduce new perspectives in science and engineering...the big picture of the project. For example, how can future regulations impact your work? What design ... -
Thanks to Addgene Depositors, We’re Now Sharing over 100,000 Reagents!
TypeBlog PostPublishedApril 8, 2021, 7:25 p.m.... stats and more on the addgene.org homepage! You can track the number of plasmids right below the search... -
Antibodies 101 Series on TikTok
TypeBlog PostPublishedMarch 19, 2024, 1:15 p.m....shared on our @addgene Instagram account, or you can watch the full animation on our YouTube channel. ...