We narrowed to 801 results for: Cre

1. AAV Q&A with Tim Miles

   Type

   Blog Post

   Published

   May 17, 2022, 1:15 p.m.

   ...flexibility of AAV genome design in remarkably creative ways. The continued development of more and smaller...

2. Antibodies 101: Buffers, Storage, and Conjugates

   Type

   Blog Post

   Published

   Feb. 1, 2022, 2:15 p.m.

   ...basic guidelines below, you’ll likely be able to create a small, usable panel.  First, fluorophores vary...

3. Antibodies 101: Isotypes

   Type

   Blog Post

   Published

   Oct. 19, 2021, 1:15 p.m.

   ... IgG; but it's the most prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects...

4. From Lab Bench to Leadership: Transitioning into Management as a PhD

   Type

   Blog Post

   Published

   Jan. 21, 2025, 2:15 p.m.

   ...leadership approach to each person, I was able to create a more productive and cohesive team environment...

5. GCE4All: Making Genetic Code Expansion Accessible

   Type

   Blog Post

   Published

   June 15, 2023, 1:15 p.m.

   ...The catch The rapid expansion of GCE led to the creation of many new tools and potential applications. ...

6. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

   Type

   Blog Post

   Published

   May 11, 2017, 2:30 p.m.

   ...many major cancer genes. These should be used to create ever more complete “look-up-tables” of mutant function...

7. Antibodies 101: Flow Cytometry Controls

   Type

   Blog Post

   Published

   June 6, 2024, 1:50 p.m.

   ...cells but with far more predictability. The beads create an artificial positive (bound) and negative (unbound...

8. Plasmids 101: Methylation and Restriction Enzymes

   Type

   Blog Post

   Published

   June 30, 2016, 2:30 p.m.

   ...keep in mind that dam-/dcm- strains may have an increased rate of mutation (as these would also be deficient...

9. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

   Type

   Blog Post

   Published

   Feb. 10, 2016, 3:30 p.m.

   ...harnesses the power of bacterial recombineering to create insertions, deletions, and substitutions - at the...

10. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    Published

    April 16, 2015, 3:45 p.m.

    ...Genome-Wide Control for Large-Scale Functional Screens        ...

11. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type

    Blog Post

    Published

    Feb. 4, 2014, 3:50 p.m.

    ...can make a big difference, if you can find one. Create your own peer mentoring circle. This type of program...

12. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    Published

    March 19, 2019, 1:31 p.m.

    ...toolbox, Ed Boyden’s lab developed a way to rapidly screen thousands of proteins for one that can report electrical...

13. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type

    Blog Post

    Published

    Feb. 28, 2019, 1:34 p.m.

    ...highlights of 2018 for me was to collaborate with this incredibly dedicated team. Many thanks to Tyler Ford, Caroline...

14. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type

    Blog Post

    Published

    Dec. 8, 2022, 2:15 p.m.

    ...limitations. New efforts have recently been made to increase cutting efficiency and diversify PAM sequences...

15. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type

    Blog Post

    Published

    April 23, 2018, 2 p.m.

    ...inhibition. To assess TRIM21 toxicity, Clift et al. created stable cell lines overexpressing TRIM21, finding...

16. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...proteins are available, and their number is steadily increasing. Of course, you want the ‘latest and greatest...

17. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type

    Blog Post

    Published

    Dec. 8, 2016, 3:30 p.m.

    ...more efficient, as one does not have to thoroughly screen colonies for the insert. Gateway® technology (developed...

18. Visualizing Protein Turnover In Situ

    Type

    Blog Post

    Published

    Jan. 16, 2018, 3:20 p.m.

    ...neurodegeneration. The level of this protein is decreased in Alzheimer’s disease patient’s brains. However...

19. Antibodies 101: Conjugation

    Type

    Blog Post

    Published

    March 26, 2024, 1:15 p.m.

    ...protein-protein interactions with other peptides. Created with BioRender.com.     Many biologists can...

20. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type

    Blog Post

    Published

    May 11, 2021, 1:15 p.m.

    ...Roth BL (2007) Evolving the lock to fit the key to create a family of G protein-coupled receptors potently...