We narrowed to 798 results for: Cre

1. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

   Type

   Blog Post

   Published

   May 11, 2017, 2:30 p.m.

   ...many major cancer genes. These should be used to create ever more complete “look-up-tables” of mutant function...

2. From Lab Bench to Leadership: Transitioning into Management as a PhD

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   Blog Post

   Published

   Jan. 21, 2025, 2:15 p.m.

   ...leadership approach to each person, I was able to create a more productive and cohesive team environment...

3. GCE4All: Making Genetic Code Expansion Accessible

   Type

   Blog Post

   Published

   June 15, 2023, 1:15 p.m.

   ...The catch The rapid expansion of GCE led to the creation of many new tools and potential applications. ...

4. Antibodies 101: Flow Cytometry Controls

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   Blog Post

   Published

   June 6, 2024, 1:50 p.m.

   ...cells but with far more predictability. The beads create an artificial positive (bound) and negative (unbound...

5. Plasmids 101: Methylation and Restriction Enzymes

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   Blog Post

   Published

   June 30, 2016, 2:30 p.m.

   ...keep in mind that dam-/dcm- strains may have an increased rate of mutation (as these would also be deficient...

6. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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   Blog Post

   Published

   Feb. 10, 2016, 3:30 p.m.

   ...harnesses the power of bacterial recombineering to create insertions, deletions, and substitutions - at the...

7. CRISPR 101: Non-Homologous End Joining

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   Blog Post

   Published

   April 16, 2015, 3:45 p.m.

   ...Genome-Wide Control for Large-Scale Functional Screens        ...

8. Will You Be My Mentor? Finding and Asking for Mentoring Support

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   Blog Post

   Published

   Feb. 4, 2014, 3:50 p.m.

   ...can make a big difference, if you can find one. Create your own peer mentoring circle. This type of program...

9. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

   Type

   Blog Post

   Published

   May 11, 2021, 1:15 p.m.

   ...Roth BL (2007) Evolving the lock to fit the key to create a family of G protein-coupled receptors potently...

10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    Published

    March 19, 2019, 1:31 p.m.

    ...toolbox, Ed Boyden’s lab developed a way to rapidly screen thousands of proteins for one that can report electrical...

11. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type

    Blog Post

    Published

    Feb. 28, 2019, 1:34 p.m.

    ...highlights of 2018 for me was to collaborate with this incredibly dedicated team. Many thanks to Tyler Ford, Caroline...

12. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type

    Blog Post

    Published

    April 23, 2018, 2 p.m.

    ...inhibition. To assess TRIM21 toxicity, Clift et al. created stable cell lines overexpressing TRIM21, finding...

13. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...proteins are available, and their number is steadily increasing. Of course, you want the ‘latest and greatest...

14. Antibodies 101: Conjugation

    Type

    Blog Post

    Published

    March 26, 2024, 1:15 p.m.

    ...protein-protein interactions with other peptides. Created with BioRender.com.     Many biologists can...

15. Visualizing Protein Turnover In Situ

    Type

    Blog Post

    Published

    Jan. 16, 2018, 3:20 p.m.

    ...neurodegeneration. The level of this protein is decreased in Alzheimer’s disease patient’s brains. However...

16. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type

    Blog Post

    Published

    Dec. 8, 2016, 3:30 p.m.

    ...more efficient, as one does not have to thoroughly screen colonies for the insert. Gateway® technology (developed...

17. CRISPR 101: Cas9 vs. The Other Cas(s)

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    Blog Post

    Published

    Dec. 8, 2022, 2:15 p.m.

    ...limitations. New efforts have recently been made to increase cutting efficiency and diversify PAM sequences...

18. Working with Nuclear Receptors

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    Blog Post

    Published

    July 15, 2014, 4:03 p.m.

    ... double-edged sword, however, and has greatly increased the burden associated with identifying plasmids...

19. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type

    Blog Post

    Published

    May 3, 2022, 1:15 p.m.

    ...acetylation system and novel in vivo high-throughput screen for molecules affecting alpha-synuclein oligomerisation...

20. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type

    Blog Post

    Published

    July 12, 2021, 1:15 p.m.

    ...Hsu PD (2021) Genome-wide, bidirectional CRISPR screens identify mucins as critical host factors modulating...