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...arms. When we select cells later, we will first perform a positive selection for the antibiotic resistance...

...scanning microscopy . Since image acquisition is performed using cameras with electron multiplying couple-charged...

...Guide for more molecular biology and cloning information. ...

...initial guidelines I received from the teachers, I formulated the presentation as follows (which could be adapted...

...this article from The Muse. Kai Strickland is a former Customer Support and Operations Manager at Addgene...

...Neveu PA. MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian...

...number of photons before bleaching. The amount of information one can extract from a biological sample is wholly...

...titers due to higher plasmid number. For more information on the key differences between the 2nd and 3rd...

...overhang 18-23 bases away from the PAM. With this information, they turned to a cell culture system to see ...

...strategy builds on the steps listed above and formalizes your development. You may consider earning a ...

...of new technologies may be very effective in transforming conventional research. So, I hope my younger...

...Genetic Tools Atlas (GTA). The GTA has a wealth of information and includes annotated images of the brain sections...

...dilute the unintegrated plasmids (Fig. 2b). Next, perform a short (12-hour) induction with doxycycline prior...

...ccdB gene die off and a higher percentage of transformants contain the ccdB-less construct. Other toxin-antitoxin...

...identified (Hart et al, Bassik et al). The library performed well in both screens, with the growth screen having...

...genes in mice. For both genes, they saw indel formation and phenotypic changes as early as 1 week post-injection...

...in the laboratory mouse, most optogenetics is performed in this species. However, transgenic rat technology...

...Since then FRET has also been used to measure conformational changes, cleavage activity using FRET-based...

...Iwakawa, H., & Tomari, Y. (2021). Life of RISC: Formation, action, and degradation of RNA-induced silencing...

... very excited to set up a CRISPR-based editing platform in our lab. Our goal was to use the system to ...