We narrowed to 756 results for: form
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TypeBlog PostPublishedNov. 1, 2018, 12:35 p.m....ccdB gene die off and a higher percentage of transformants contain the ccdB-less construct. Other toxin-antitoxin...
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Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m....genes in mice. For both genes, they saw indel formation and phenotypic changes as early as 1 week post-injection... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog PostPublishedJan. 24, 2023, 2:15 p.m....dilute the unintegrated plasmids (Fig. 2b). Next, perform a short (12-hour) induction with doxycycline prior... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog PostPublishedOct. 1, 2015, 2:30 p.m....in the laboratory mouse, most optogenetics is performed in this species. However, transgenic rat technology... -
Tips for Using FRET in Your Experiments
TypeBlog PostPublishedNov. 5, 2014, 3:41 p.m....Since then FRET has also been used to measure conformational changes, cleavage activity using FRET-based... -
Five Popular Model Organisms
TypeBlog PostPublishedApril 11, 2019, 12:39 p.m....extrachromosomal maintenance and integration of transforming sequences." The EMBO journal 10.12 (1991): 3959... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog PostPublishedDec. 2, 2019, 2 p.m....proteins from a complex of interest. For more information, read the Split-BioID blog post. RaPID: RNA-... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog PostPublishedSept. 5, 2017, 1:58 p.m.... very excited to set up a CRISPR-based editing platform in our lab. Our goal was to use the system to ... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog PostPublishedJune 17, 2025, 1:15 p.m....Iwakawa, H., & Tomari, Y. (2021). Life of RISC: Formation, action, and degradation of RNA-induced silencing... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog PostPublishedSept. 1, 2015, 2:30 p.m....find personnel salary ranges, go to online job information sites (like, indeed.com, or salary.com). Search... -
Plasmids 101: Repressible Promoters
TypeBlog PostPublishedDec. 20, 2022, 2:15 p.m....recent variants of the Tet-Off system. For more information on Addgene Tet plasmids, see our Tetracycline... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog PostPublishedOct. 27, 2020, 1:15 p.m....separated by a loop region that facilitates the formation of a hairpin RNA structure, resulting in processing... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog PostPublishedOct. 17, 2019, 12:28 p.m....technology. Read the genOway press release for more information. References Abdallah, Khaled, et al. "Adeno-associated... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog PostPublishedAug. 15, 2017, 1:24 p.m....nodes. These methods gave researchers much more information regarding immune cell mobility than the static... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m....Class 2 CRISPR systems called CasPEDIA, with information about enzyme activity, experimental conditions... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog PostPublishedNov. 6, 2018, 1:12 p.m....cell to revert back to a pluripotent stage (iPSC formation) [2, 3]. The iPSCs then proliferate and redifferentiate...