We narrowed to 904 results for: CAN

...smartphone apps you can use in the lab. Whether you’re tired of calculating dilutions or you can’t quite remember...chemical in your obscure growth medium, these apps can help make your lab life a bit easier. Since the post...University of Alberta and describes a technique you can use to find phage proteins that bind to your bacterium...phages are viruses that infect bacteria and that can do so with exquisite specificity (check out our interview... dress up as Mr. Incredible, an added bonus. You can find the rest of our September posts in chronological...post highlights a rare disease, Fibrodysplasia ossificans progressiva (a.k.a. FOP), that, while affecting...Chemistry & Small Molecule Fluorophores Addgene's Cancer Collection Pages Connect the Research Community...

...should be no secret that one of the best things you can do during your training is meet interesting people...a job. Ask openly to be connected to someone who can help you land that position such as a direct connection...Interviews are best conducted in person. Perhaps you can offer to buy coffee or lunch at a place convenient...and disappointments. These more personal questions can elicit the most valuable insights you will get from...each Informational Interview with the question, “Can you connect me to anyone else you think I should ...5-12 years worth!) with academic scientists who can’t help us learn about the nonacademic sphere where...

...email reminders. These programs can be synced to your online calendar and can be accessed from your computer...Organizing your lab notebooks, protocols, and to do lists can be easier than you think! Here are three tips to ... up out of the top of the notebooks, so that you can easily spot the colors and text. Tip 2: Use electronic...from anywhere, but also allow you to control who can edit the documents and track the revision history...travel, and you can’t remember where you put your flow cytometry protocol. Or maybe you can’t find the molecular...

...competition, where you can get all your burning AAV questions answered.  Interested? You can learn more here ...travel prize in our open data extravaganza!  Anyone can submit data from their CLOVER lab or Addgene AAV ...

...editing tool. However, Cas9’s endonuclease activity can be limited by the need for a specific PAM (protospacer...bind the target sites for editing. PAM sequences can be from 2-6 base pairs in length and follow the DNA...versatility of the engineered SpCas9-NG and its variants can be used to engineer specific sites that were previously...Anion-conducting channelrhodopsins (ChR) are ion channels that can be used to silence the activity of neurons. Earlier...cysteine.  With the introduction of MerMAIDs, neurons can be turned off quickly and with high precision - a... Anchor. Using specifically designed gRNAs, they can then recruit these LADL Anchors to genomic targets...

...Temperature: You can place your reaction on ice if you are planning to transform right away OR you can store the...group from a different DNA strand comes along, it can attack this covalent bond thus joining the two DNA... TOPO Cloning Reaction into Competent Cells: You can use your standard lab protocol for this; however,...and Analyze 10 White or Light Blue Colonies: You can confirm the presence of your insert by PCR, restriction...when the PCR product and vector ligate; however, it can potentially rebind and nick the newly ligated DNA...minutes (incubating the full 30 minutes will not significantly improve the transformation efficiency).  5. ...

...getting buy-in when delegating can help ensure successful results.  Can you provide the information and...you are giving this responsibility to someone who can do the job. It is good to delegate so the person ...result in more engaged, successful employees who can accomplish great things.   To read more on this ... excellent blog on delegation tips and practices can be found at the Time Doctor Blog "The Art of Delegation... How Volunteering at a Professional Organization Can Help You Develop Leadership Skils Set Concrete Goals...manager must do. You can’t delegate the giving of feedback or performance review. You can’t delegate tasks ...

...Diagnostic restriction digest Diagnostic digests can be used to confirm the rough structure of the plasmid...features within the plasmid. Restriction analysis can also be used successfully even if you don't have ... Once you have purified plasmid DNA, this method can be done right in your lab in less than a day. Diagnostic...as well as the predicted size of the insert. You can search NCBI for YGOI to find the particular reference...the dyes provide a visual reference point so you can easily assess how far the gel has run. Bonus: The...The dyes also run at predicted sizes so you can estimate how far down the gel your bands have traveled ...

...strong problem solving skills alone can take you - just because I can resolve issues effectively does not...problem solving skills” and look at how scientists can develop and highlight these valuable skills. Hopefully...found that taking a conscious or active approach can be hugely helpful not only for developing a skill...entire problem solving process on your own, but you can start by paying attention to how your mentors reach...solutions and ask them to explain their logic. Or you can try applying the scientific method to everyday problems...positions. Highlighting your problem solving skills can help convince a future employer that you are well...

...suitable for both short-and long-lived proteins, and it can even distinguish between protein variants when used...antibody works well. In larger cell populations, you can deliver these components together using electroporation...protein knockdown experiments! The TRIM-Away plasmids can be found here. Clift et al. Cell. 2017. PubMed PMID...which sgRNAs are used with FIRE-Cas9, experiments can be designed to target multiple loci at once. In addition...memory at nearly any locus. The FIRE-Cas9 plasmids can be found here. Braun et al. Nat Communications. ...encoding synthetic yeast transcription factors that can independently control expression of your genes of...progesterone do not affect each other, these systems can be used together. Aranda-Diaz et al additionally ...