Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 790 results for: Cre

Showing: 381 - 390 of 790 results

  1. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...inefficient, and often not reproducible. Prior to creating transgenic cultures or plants, our transient expression... expression method enables us to quickly screen and investigate transcription factor candidates and the...with the MoClo system you can easily and quickly create customized vectors. For more information on MoClo...transient leaf transformations are a great help in screening candidate genes and speeding up research. Certainly...

  2. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ... sequences Restriction sites Sequences prone to create secondary structures in RNA transcripts Effects...factors on their own. Luckily, many researchers have created codon optimization algorithms and DNA synthesis...abundance tRNAs in your expression host and thereby increase their abundance. For example, the commercial Rosetta...system for many different genes without having to create new constructs. However, due to problems such as...

  3. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...s clinical and commercial future, and yet are incredibly difficult to find solutions to. There are many...on them in a timely fashion while allowing for creative solutions to emerge. They enjoy the fact that ...home and at the office. Consulting firms are increasingly aware of the toll that long working hours and...career paths available to MDs and PhDs, and an increasing recognition of the value graduates bring to these...

  4. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ... table below lists plasmids that can be used to create your choice of fluorescent fusion protein with ...quenching and the fluorescence of the donor is increased. This method is straightforward and quantitative...days and good “gigs” ahead...No offense to BRET, CRET and other RET techniques which have never outclassed... BRET= Bioluminescence Resonance Energy Transfer CRET= Chemiluminescence Resonance Energy Transfer    ...

  5. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...most unique model organisms, recognized for its incredible regeneration abilities. The axolotl has the ability..., 2019). To study regeneration scientists have created molecular biology tools to generate transgenic ...Xenopus genes and a tool-kit from the Hamdoun Lab to create fluorescent fusions in a multitude of organisms...Xing, X.-H. (2018). Pooled CRISPR interference screening enables genome-scale functional genomics study...

  6. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ... an organization gets, and creating new avenues of communication can create as many problems as it can...guidance X and Y might be able to work together to create solution C. And if a conflict resolver does their...have learned that by working together, they can create unique solutions that benefit themselves and their...

  7. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...21–26). Increased scientific funding (17). Federal funding for science has been decreasing for the last... government and, consequently, politics. More concretely, scientists gather information about reality ...institutions, while many also seek to honor and credit women, immigrants, and minorities who have had ...

  8. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...complex can bind to your biotinylated antibody. This creates a large meshwork of biotin-avidin complexes bound...label a protein of interest with biotin by simply creating a fusion in which AviTag is added to either the...or polypeptides to a solid substrate (Holden and Cremer, 2003). References 1. Chapman-Smith, A., & Cronan...588–600. PubMed PMID: 2388593 7. Holden, M. A., & Cremer, P. S. “Light activated patterning of dye-labeled...

  9. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...phosphoserine (Sep)-tRNA synthetase enabled researchers to create an orthogonal translation system (OTS) for the ...residues in the above study with phosphoserine (to create a pSer/phosphomimetic dual mutant) elevated kinase... as an alternative to phosphomimetic mutation. Creating phosphoserine-incorporated proteins The generation... This is a particularly important control when creating a phosphoserine-incorporation mutant for a residue...

  10. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...Experimental output is measured and quantified. Created with BioRender.com.   Optogenetic tools Types...passing ions against the concentration gradient. Created with BioRender.com.   Opto-G-protein coupled...the binding pocket and activates the receptor. Created with BioRender.com.   Controlling secondary...LOV domain allowing the pathway to be activated. Created with BioRender.com.   Important considerations...
Showing: 381 - 390 of 790 results