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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 793 results for: KIN

Showing: 381 - 390 of 793 results

  1. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...nLightR greatly expand the toolbox for researchers looking to study norepinephrine, the Patriarchi lab continues...Ovchinnikov, S., & Steinegger, M. (2022). ColabFold: Making protein folding accessible to all. Nature Methods...Technique is Best for Me?  In Living Color: The Skinny on In Vivo Imaging Tools Resources on Addgene....

  2. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...because it finally feels to me like the postdocs are taking some steps to control their own future in science...speaker, Dr. Henry Bourne (shown in photo), has been speaking out and writing about issues in our science training...experience and encouragement.  Dr. Sibby Anderson-Thompkins, Director of the Office of Postdoctoral Affairs...

  3. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    Published
    Aug. 13, 2014, 3:49 p.m.
    ...Streptococcus and the “py” in pyogenes), capable of locking two peptide tags together. SpyLigase overcomes ...forces acting on them. For the application we were looking at, magnetic isolation of circulating tumor cells..., highly stable and has this amazing chemistry locking the domain together - no one knows what for. Many...

  4. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ... no fear - plasmids from Addgene depositors are making it easier than ever to use CRISPR  in E. coli and...gene body can repress transcription by physically blocking the elongation complex from binding the DNA or...Patino R, Heussler GE, Hearne CC, Qu J, Inclan YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters... Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA (2013) Repurposing CRISPR as an RNA-Guided...

  5. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...background noise. This is not terribly problematic when working with cell culture, because it is easy to harvest...harvest large quantities of cells. However, when working on specific tissues or organs in whole animal models...major technical hurdle.   ChIP-seq requires  cross-linking during an initial fixation step. This serves to...

  6. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    ... cells containing the plasmid construct you’re seeking. Fortunately, there are many ways to do this involving... a successful uptake of your DNA of interest by taking advantage of bacterial characteristics such as ...for specific amino acids. When plated on media lacking the essential amino acid, the bacteria will only...

  7. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...also involved in reinforcement learning, decision making, and motor control.  What do we understand about... 100 nm) of DA. In addition, they exhibit fast kinetics (10 ms on and 100 ms off), enabling detection ...magnitudes of fluorescent responses. Studies on the kinetics of these sensors showed rapid response (60 or ...

  8. Oh, The Places You Can Go: Careers in Science Communication - Product Development

    Type
    Blog Post
    Published
    Oct. 18, 2017, 1 p.m.
    ...bunch. They don’t really know where I came from.” Working at eScience Labs, an educational company that creates...that provides scientific editing services. “I was working during the day and editing at night. It was not...moved out here, I was still editing from home and looking for a job.” But Caitlin’s persistence paid off:...

  9. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...fluorescent signal and enables single molecule tracking  within living cells without affecting protein...managed to track run lengths of the motor protein kinesin across microtubules. Tanenbaum et al. then tested...photobleaching Simplified, long-term single-molecule tracking Caveats of SunTag Very large size: a 24x scaffold...

  10. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...Especially when your collaboration includes scientists working in different time zones, it’s important that everyone...accountable to these expectations once they’re set by checking in about them at your recurring meetings and reaching...common. I hope these tips are useful whether you’re looking to improve communication within an existing collaboration...
Showing: 381 - 390 of 793 results