... Here at Addgene we’re getting into the holiday spirit and we hope you are, too! Bluegene is eager to...and tweeting it with the tags #DeckTheLab and @Addgene. Christmas, Hanukkah, ugly sweaters, Flannel Fridays...lucky winner? Happy holidays from all of us at Addgene! We look forward to seeing how you #DeckTheLab!...

... are essential for processes such as gluconeogenesis, lipogenesis, amino acid metabolism and energy transduction...transduction. Biotinylation is the general process by which biotin is added to a protein or macromolecule... plasmids that contain both AviTag and BirA at Addgene! Oligonucleotide labeling Biotin can be added to...PubMed PMID: 8245422. Additional resources on the Addgene blog In vivo biotinylation of bacterial fusion...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Additional Resources on the Addgene Blog Check out additional microbiology resources Stem cell models for...additional plant biology blog posts Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ...

...particularly genetics research, for decades. Its short life cycle, reasonably close genetic homology to...demonstrate the new system in a video filmed at Addgene:     If you're working in fly research, I hope...References and Resources Additional resources on the Addgene blog:  Flies 101: Catching flies in the lab References...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...research and to keep useful discoveries coming. At Addgene, we’re huge supporters of science communication...Additional Resources on the Addgene Blog Check Out our Interview with Science Rapper Tom McFadden Learn about...

...We’re big fans of all our depositors here at Addgene — your contributions make the repository what it... designing a novel topology, then iteratively generated 3D models and amino acid sequences that got closer...Baker Lab has deposited multiple Top7 plasmids at Addgene, along with other tools like high resolution Ras...crosslinked peptides proposed as a scaffold for a new generation of drugs, all developed with their protein design...

...signs of holiday spirit still linger on around the Addgene office as we peruse the #DeckTheLab submissions...MerryChristmas #happyholidays2018 #DeckTheLab @Addgene @joachimgoedhart @SAbiega @EikeMahlandt @Saskia_deMan... @EmoryChem lab office looking festive 🎄🌟🎁 @Addgene #DeckTheLab pic.twitter.com/bDRqAKrWHz — Dyer Lab...winners will receive a holiday care package from Addgene. We hope all of our entrants had fun decorating...

...resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the Addgene blog Learn...approaches for lab sustainability Resources on Addgene.org Sign up for our quarterly newsletter Help other...resources by sharing plasmids Find plasmids at Addgene ...

... event – all while keeping the party going! At Addgene, we’ve set-up an exhibitor booth in order to help...all the words of support from scientists using Addgene in their labs. And it has been exciting to learn... I heard an impressive talk about the use of optogenetics tools to inhibit epileptic seizures in a mouse...McGovern Institute for Brain Research) – and an Addgene depositing scientist – stop by our booth to say...

...KTRs) Kinase Translocation Reporters (KTRs) are genetically encoded fluorescent kinase reporters. A KTR is...custom KTR design, plasmids are available from Addgene! References Kudo, T., Jeknić, S., Macklin, D.N...KTR plasmids here! Additional Resources on the Addgene Blog Learn about photoswitchable kinases Learn...protein phosphorylation Additional Resources on Addgene.org Find FRET plasmids Find fluorescent biosensors...

...fluorescent microscopy technique for your study see Addgene’s blog post Which Fluorescence Microscopy Techniques...experiment and are unsure which to choose, check out Addgene’s article: Which Fluorescent Protein Should I Use.... During FACS, the sample stream oscillates to generate droplets that are charged as they pass through.../s10616-011-9415-0 Additional resources on the Addgene blog Check out other methods using fluorescent...proteins Learn more about antibodies Resources on Addgene.org Visit the Fluorescent Protein Guide Find antibody...

...Resources on Addgene.org Addgene's Antibody Guide Ready-to-use antibodies in the Addgene repository Antibody...fluorescent reporter, such as GFP or mCherry, through genetic modification. As your protein of interest will ...sailing, and reading.   More resources on the Addgene blog Antibodies 101: Introduction to Gating in ...

...the dye and the endogenous target, and thus no genetically encoded tag is required. These dyes can also ...be SNAP-tag® and HaloTag®. These consist of a genetically encoded enzyme variant tag that covalently reacts...maintained through mechanisms like fission, fusion, biogenesis, and mitophagy, can be tracked using dyes like...Keller, P. J., Ji, N., & Lavis, L. D. (2017). A general method to fine-tune fluorophores for live-cell ....1016/j.bbamem.2013.09.005 More resources from Addgene Fluorescent protein plasmids and resourcesA practical...

...resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the Addgene blog Find...Find lab tips for your research Resources on Addgene.org Sign up for our quarterly newsletter Help other...resources by sharing plasmids Find plasmids at Addgene ...

...power of community in 2024 In 2024, your trust in Addgene helped unlock unprecedented scientific collaboration...sequencing capabilities to complement our next-generation sequencing, setting new standards for plasmid...online and in-person, you helped us understand how Addgene can better serve the global scientific community...about our latest developments? Subscribe to the Addgene newsletter. References Ayoubi, R., Ryan, J., Gonzalez...

...protein. The pCri system, recently deposited to Addgene by F. Xavier Gomis-Rüth, aims to take the guesswork... either individually or as a complete kit from Addgene. References 1. Goulas, Theodoros, et al. "The ...visualization of the data from RSCB PDB Resources on the Addgene Blog Plasmids 101: E. coli strains for protein...expression Plasmids 101: Protein tags Resources on Addgene.org Find Additional Cloning Kits ...

...When I started writing for the Addgene blog, I was focused on writing about new scientific techniques...first program I used to create graphics for the Addgene blog. However, it has a limited number of drawing...body, cellular biology, medical specialities, and general items. Individual images as well as collections...comments section!  Additional resources on the Addgene blog Read our Early Career Researcher Toolbox ...

...vary. In the PAM-out configuration, D10A tends to generate small deletions, while H840A is biased towards... likely due to the distinct overhang patterns generated by the nickases; D10A creates 5’ overhangs and...reviewing this blog post. Additional Resources on the Addgene Blog Learn about Homology Directed Repair 3 Tips...Efficiency How to Design Your gRNA Resources on Addgene.org Check out CRISPR Topic Page Find CRISPR Nickase...

...scientists here Resources on Addgene.org Learn more about careers at Addgene ...posts in this series! Additional Resources on the Addgene Blog Learn more about alternative science careers...creative science education tools? Check out this Addgene podcast with educator and science rap mastermind...

...further consolidate these FAQs, Le Cong worked with Addgene Senior Scientist, Matt Ferenc, to organize the ...full list of 16 FAQs answered by Le Cong Read Addgene's CRISPR guide for background information on CRISPR...the most frequently requested CRISPR plasmids at Addgene Find protocols and gRNA design tools: List of...scientific community and sharing these resources with Addgene. Thank you, Le!    ...