We narrowed to 781 results for: Por

1. DNA Purification Without a Kit

   Type

   Blog Post

   Published

   Feb. 11, 2020, 2:15 p.m.

   ...Purification and Gel Extraction Kits. Scientific Reports. https://doi.org/10.1038/s41598-018-30316-w  Zou...

2. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

   Type

   Blog Post

   Published

   Dec. 3, 2019, 11:38 a.m.

   ... let us know! Contact our scientific technical support at [email protected] and let us help.  Check back...

3. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

   Type

   Blog Post

   Published

   Oct. 4, 2018, 12:44 p.m.

   ... enough for efficient library performance, an important criterion for probing a limited number of cells...

4. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

   Type

   Blog Post

   Published

   Aug. 30, 2022, 1:15 p.m.

   ... for antibodies that bind to the extracellular portion of a protein. B) Permeabilizing with the selective...

5. The PAM Requirement and Expanding CRISPR Beyond SpCas9

   Type

   Blog Post

   Published

   Aug. 20, 2020, 2:30 p.m.

   ...Hu et al., 2018).  In 2020, the Kleinstiver lab reported the development of near-PAMless Cas9 variants....

6. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

   Type

   Blog Post

   Published

   Nov. 1, 2018, 12:35 p.m.

   ...concept, they tested a lux-mediated bioluminescence reporter in vivo in the absence of selective pressure and...

7. Plasmids 101: Codon usage bias

   Type

   Blog Post

   Published

   Sept. 27, 2018, 1:09 p.m.

   ...isoacceptors induced by amino‐acid starvation." EMBO reports 6.2 (2005): 151-157. PubMed PMID: 15678157. PubMed...

8. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

   Type

   Blog Post

   Published

   Sept. 13, 2018, 1:55 p.m.

   ... Screens Before diving into the results, it’s important to note that in Morgens et al., guides were considered...

9. Neuronal labeling with Spaghetti Monster

   Type

   Blog Post

   Published

   Aug. 14, 2018, 12:42 p.m.

   ... and fluorescent proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal...

10. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type

    Blog Post

    Published

    July 23, 2019, 12:46 p.m.

    ...The GPI-Linked Protein LY6A Drives AAV-PHP. B Transport across the Blood-Brain Barrier." Molecular Therapy...

11. Tips for CRISPR Gene Editing in Mice

    Type

    Blog Post

    Published

    June 28, 2016, 10:59 a.m.

    ...expressing Cas9 and single guided RNA." Scientific reports 3 (2013). PubMed PMID: 24284873. PubMed Central...

12. Antibodies 101: Beyond Surface Labeling

    Type

    Blog Post

    Published

    June 27, 2024, 1:15 p.m.

    ...bromodeoxyuridine (BrdU) can be used. These dyes are incorporated into the DNA during DNA synthesis and then diluted...

13. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type

    Blog Post

    Published

    March 1, 2016, 3:30 p.m.

    ...primers for PCR amplification of fragments to incorporate such regions of homology. This video gives a ...

14. A Control for All Seasons

    Type

    Blog Post

    Published

    Oct. 5, 2023, 1:15 p.m.

    ...binding  Primary antibody specificity is further supported by a pre-immune serum or isotype-specific control...

15. Antibodies 101: The Basics of Western Blotting

    Type

    Blog Post

    Published

    March 8, 2021, 2:41 p.m.

    ...while less protein leads to a thinner band. It is important to note, however, that you must include a loading...

16. Technique: Probe Phage Genomes for Host Binding Proteins

    Type

    Blog Post

    Published

    Sept. 29, 2016, 2:30 p.m.

    ...to probing with cells in order to optimize this portion of the assay before attempting it with a random...

17. Deep Dive: Statistical Tests (Comparisons)

    Type

    Blog Post

    Published

    March 7, 2023, 2:15 p.m.

    ...most effective and accurate way possible it’s important to select the right test for the job (and, uh,...

18. When Fidelity Matters: A frank discussion about ligase fidelity

    Type

    Blog Post

    Published

    March 6, 2018, 1:49 p.m.

    ...commercially available (see below). Conclusion It is important to note that thermostable, high fidelity, nick-selective...

19. Antibodies 101: Introduction to Antibodies

    Type

    Blog Post

    Published

    Jan. 19, 2021, 2:15 p.m.

    ...target protein: instead, it recognizes a small portion of the protein, known as an epitope. Epitopes are...

20. CRISPR 101: Cytosine Transversion Editors

    Type

    Blog Post

    Published

    March 25, 2025, 1:15 p.m.

    ...Adenine Base Editors Read about four base editor reporters CRISPR 101: Prime Editing CRISPR 101: Cas9 Structural...